##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AccIII AclI Alw44I Aor51HI
ApaLI AscI AseI AsnI AvrII
BamHI BcgI BclI BglII BlnI
BsaBI BseAI BsiWI BspDI BspEI
BspHI BspLU11I BspMI BsrDI Bst1107I
BstBI BstZ17I ClaI DraI DraIII
Eco47III EcoT22I FbaI HpaI MamI
MfeI MluI MroI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RcaI
SacI SalI SfiI SfuI SnaBI
SpeI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2847 GACGTC GACGT^C
Acc65I 3778 GGTACC G^GTACC
AflII 921 CTTAAG C^TTAAG
AgeI 1621 ACCGGT A^CCGGT
AhdI 1575 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 342 CCTGAGG CC^T[ACGT]AGG
Asp700I 1619 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3778 GGTACC G^GTACC
AspEI 1575 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2566 TGCGCA TGC^GCA
BbeI 2657 GGCGCC GGCGC^C
BbsI 2160 GAAGAC GAAGAC(2/6)
BciVI 2105 GGATAC GTATCC(6/5)
BfrI 921 CTTAAG C^TTAAG
BpuAI 2160 GAAGAC GAAGAC(2/6)
BsmI 1715 GAATGC GAATGC(1/-1)
BstAPI 3173 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2394 GGTCACC G^GT[ACGT]ACC
BstPI 2394 GGTCACC G^GT[ACGT]ACC
Bsu36I 342 CCTGAGG CC^T[ACGT]AGG
CpoI 90 CGGTCCG CG^G[AT]CCG
Eam1105I 1575 GACGGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 342 CCTGAGG CC^T[ACGT]AGG
EcoNI 3315 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2394 GGTCACC G^GT[ACGT]ACC
EcoRV 1542 GATATC GAT^ATC
FseI 258 GGCCGGCC GGCCGG^CC
FspI 2566 TGCGCA TGC^GCA
HindIII 3435 AAGCTT A^AGCTT
KasI 2657 GGCGCC G^GCGCC
KpnI 3778 GGTACC GGTAC^C
KspI 326 CCGCGG CCGC^GG
NarI 2657 GGCGCC GG^CGCC
NotI 322 GCGGCCGC GC^GGCCGC
NruI 46 TCGCGA TCG^CGA
PflMI 590 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1621 ACCGGT A^CCGGT
PshAI 1761 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 90 CGGTCCG CG^G[AT]CCG
SacII 326 CCGCGG CCGC^GG
SapI 1142 GCTCTTC GCTCTTC(1/4)
SbfI 2306 CCTGCAGG CCTGCA^GG
ScaI 1132 AGTACT AGT^ACT
SexAI 1912 ACCTGGT A^CC[AT]GGT
SfoI 2657 GGCGCC GGC^GCC
SgrAI 57 CGCCGGTG C[GA]^CCGG[CT]G
Sse8387I 2306 CCTGCAGG CCTGCA^GG
StuI 1945 AGGCCT AGG^CCT
Van91I 590 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1619 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 1077 GTGCTC G[AT]GC[AT]^C
3288 GTGCTC
AspI 2271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3506 GACAAAGTC
BbrPI 2621 CACGTG CAC^GTG
2865 CACGTG
BbvCI 2115 CCTCAGC CCTCAGC(-5/-2)
1643 GCTGAGG
BlpI 154 GCTCAGC GC^T[ACGT]AGC
3055 GCTAAGC
Bpu1102I 154 GCTCAGC GC^T[ACGT]AGC
3055 GCTAAGC
BsiHKAI 1077 GTGCTC G[AT]GC[AT]^C
3288 GTGCTC
Bsp1407I 1369 TGTACA T^GTACA
2296 TGTACA
BsrGI 1369 TGTACA T^GTACA
2296 TGTACA
BssSI 795 CACGAG CACGAG(-5/-1)
3096 CTCGTG
BstYI 338 AGATCC [GA]^GATC[CT]
1010 GGATCT
CelII 154 GCTCAGC GC^T[ACGT]AGC
3055 GCTAAGC
EagI 257 CGGCCG C^GGCCG
323 CGGCCG
EclXI 257 CGGCCG C^GGCCG
323 CGGCCG
Eco52I 257 CGGCCG C^GGCCG
323 CGGCCG
EcoRI 1251 GAATTC G^AATTC
1437 GAATTC
HincII 3489 GTCAAC GT[CT]^[GA]AC
3547 GTTGAC
HindII 3489 GTCAAC GT[CT]^[GA]AC
3547 GTTGAC
MflI 338 AGATCC [GA]^GATC[CT]
1010 GGATCT
NaeI 140 GCCGGC GCC^GGC
259 GCCGGC
NgoMIV 140 GCCGGC G^CCGGC
259 GCCGGC
PflFI 2271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3506 GACAAAGTC
PmaCI 2621 CACGTG CAC^GTG
2865 CACGTG
PmlI 2621 CACGTG CAC^GTG
2865 CACGTG
PpuMI 1226 GGGACCC [GA]G^G[AT]CC[CT]
1759 AGGACCT
SphI 1791 GCATGC GCATG^C
2608 GCATGC
SspBI 1369 TGTACA T^GTACA
2296 TGTACA
Tth111I 2271 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3506 GACAAAGTC
XhoII 338 AGATCC [GA]^GATC[CT]
1010 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XmaCI XmaI