################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI AspEI BbeI BbrPI BbvCI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoT22I FseI HpaI KasI KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 2044 GGTACC G^GTACC AcyI 2423 GACGCC G[GA]^CG[CT]C AflIII 1026 ACATGT A^C[GA][CT]GT Aor51HI 2352 AGCGCT AGC^GCT ApaI 227 GGGCCC GGGCC^C Asp718I 2044 GGTACC G^GTACC AspI 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1071 TGCGCA TGC^GCA BcgI 3901 GCAGAAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 338 GTATCC GTATCC(6/5) BclI 3180 TGATCA T^GATCA BsaHI 2423 GACGCC G[GA]^CG[CT]C BsaWI 12 TCCGGT [AT]^CCGG[AT] BspHI 3609 TCATGA T^CATGA BspLU11I 1026 ACATGT A^CATGT BsrBI 721 CCGCTC CCGCTC(-3/-3) BssSI 2388 CACGAG CACGAG(-5/-1) BstBI 282 TTCGAA TT^CGAA BstEII 1124 GGTGACC G^GT[ACGT]ACC BstPI 1124 GGTGACC G^GT[ACGT]ACC DrdI 921 GACAGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2352 AGCGCT AGC^GCT EcoO65I 1124 GGTGACC G^GT[ACGT]ACC EcoRV 3548 GATATC GAT^ATC FbaI 3180 TGATCA T^GATCA FspI 1071 TGCGCA TGC^GCA Hin1I 2423 GACGCC G[GA]^CG[CT]C HincII 1534 GTCGAC GT[CT]^[GA]AC HindII 1534 GTCGAC GT[CT]^[GA]AC KpnI 2044 GGTACC GGTAC^C NdeI 779 CATATG CA^TATG NheI 991 GCTAGC G^CTAGC NspV 282 TTCGAA TT^CGAA PaeR7I 1146 CTCGAG C^TCGAG PflFI 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1279 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 4244 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 3609 TCATGA T^CATGA SalI 1534 GTCGAC G^TCGAC SfuI 282 TTCGAA TT^CGAA SmaI 528 CCCGGG CCC^GGG SphI 2073 GCATGC GCATG^C Tth111I 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1279 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1146 CTCGAG C^TCGAG XmaCI 528 CCCGGG C^CCGGG XmaI 528 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccII 43 CGCG CG^CG 3857 CGCG Asp700I 1735 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3493 GAATTTCTTC AvrII 2786 CCTAGG C^CTAGG 4248 CCTAGG BanI 2044 GGTACC G^G[CT][GA]CC 2332 GGTGCC BglI 2756 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3960 GCCAGGGAGGC BlnI 2786 CCTAGG C^CTAGG 4248 CCTAGG BsmI 415 GAATGC GAATGC(1/-1) 1032 GCATTC BstUI 43 CGCG CG^CG 3857 CGCG BstXI 1045 CCATCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3924 CCAGAGGCATGG EcoRI 92 GAATTC G^AATTC 320 GAATTC HaeII 404 AGCGCC [GA]GCGC^[CT] 2352 AGCGCT MaeII 150 ACGT A^CGT 4072 ACGT MvnI 43 CGCG CG^CG 3857 CGCG NspI 1026 ACATGT [GA]CATG^[CT] 2073 GCATGC PshAI 611 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4239 GACACAGGTC SapI 886 GAAGAGC GCTCTTC(1/4) 2840 GCTCTTC XmnI 1735 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3493 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BamHI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII