##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI BbeI BbrPI BbvCI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoT22I FseI HpaI KasI KspI
MamI MluI MroI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2044 GGTACC G^GTACC
AcyI 2423 GACGCC G[GA]^CG[CT]C
AflIII 1026 ACATGT A^C[GA][CT]GT
Aor51HI 2352 AGCGCT AGC^GCT
ApaI 227 GGGCCC GGGCC^C
Asp718I 2044 GGTACC G^GTACC
AspI 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1071 TGCGCA TGC^GCA
BcgI 3901 GCAGAAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 338 GTATCC GTATCC(6/5)
BclI 3180 TGATCA T^GATCA
BsaHI 2423 GACGCC G[GA]^CG[CT]C
BsaWI 12 TCCGGT [AT]^CCGG[AT]
BspHI 3609 TCATGA T^CATGA
BspLU11I 1026 ACATGT A^CATGT
BsrBI 721 CCGCTC CCGCTC(-3/-3)
BssSI 2388 CACGAG CACGAG(-5/-1)
BstBI 282 TTCGAA TT^CGAA
BstEII 1124 GGTGACC G^GT[ACGT]ACC
BstPI 1124 GGTGACC G^GT[ACGT]ACC
DrdI 921 GACAGAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2352 AGCGCT AGC^GCT
EcoO65I 1124 GGTGACC G^GT[ACGT]ACC
EcoRV 3548 GATATC GAT^ATC
FbaI 3180 TGATCA T^GATCA
FspI 1071 TGCGCA TGC^GCA
Hin1I 2423 GACGCC G[GA]^CG[CT]C
HincII 1534 GTCGAC GT[CT]^[GA]AC
HindII 1534 GTCGAC GT[CT]^[GA]AC
KpnI 2044 GGTACC GGTAC^C
NdeI 779 CATATG CA^TATG
NheI 991 GCTAGC G^CTAGC
NspV 282 TTCGAA TT^CGAA
PaeR7I 1146 CTCGAG C^TCGAG
PflFI 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1279 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 4244 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3609 TCATGA T^CATGA
SalI 1534 GTCGAC G^TCGAC
SfuI 282 TTCGAA TT^CGAA
SmaI 528 CCCGGG CCC^GGG
SphI 2073 GCATGC GCATG^C
Tth111I 4049 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1279 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1146 CTCGAG C^TCGAG
XmaCI 528 CCCGGG C^CCGGG
XmaI 528 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccII 43 CGCG CG^CG
3857 CGCG
Asp700I 1735 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3493 GAATTTCTTC
AvrII 2786 CCTAGG C^CTAGG
4248 CCTAGG
BanI 2044 GGTACC G^G[CT][GA]CC
2332 GGTGCC
BglI 2756 GCCACTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3960 GCCAGGGAGGC
BlnI 2786 CCTAGG C^CTAGG
4248 CCTAGG
BsmI 415 GAATGC GAATGC(1/-1)
1032 GCATTC
BstUI 43 CGCG CG^CG
3857 CGCG
BstXI 1045 CCATCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3924 CCAGAGGCATGG
EcoRI 92 GAATTC G^AATTC
320 GAATTC
HaeII 404 AGCGCC [GA]GCGC^[CT]
2352 AGCGCT
MaeII 150 ACGT A^CGT
4072 ACGT
MvnI 43 CGCG CG^CG
3857 CGCG
NspI 1026 ACATGT [GA]CATG^[CT]
2073 GCATGC
PshAI 611 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4239 GACACAGGTC
SapI 886 GAAGAGC GCTCTTC(1/4)
2840 GCTCTTC
XmnI 1735 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3493 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MfeI MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII