##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      ApaLI       AscI        AseI        
AsnI        AspEI       BbeI        BbrPI       BbvCI       
BfrI        BglII       BlpI        Bpu1102I    BsaAI       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BsrFI       BsrGI       
BssHII      Bst1107I    BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DsaI        
EagI        Eam1105I    EclXI       Eco52I      EcoNI       
EcoT22I     FseI        HpaI        KasI        KspI        
MamI        MluI        MroI        NaeI        NarI        
NcoI        NgoMIV      NotI        NruI        NsiI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SbfI        ScaI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       2044  GGTACC               G^GTACC
AcyI         2423  GACGCC               G[GA]^CG[CT]C
AflIII       1026  ACATGT               A^C[GA][CT]GT
Aor51HI      2352  AGCGCT               AGC^GCT
ApaI          227  GGGCCC               GGGCC^C
Asp718I      2044  GGTACC               G^GTACC
AspI         4049  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1071  TGCGCA               TGC^GCA
BcgI         3901  GCAGAAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         338  GTATCC               GTATCC(6/5)
BclI         3180  TGATCA               T^GATCA
BsaHI        2423  GACGCC               G[GA]^CG[CT]C
BsaWI          12  TCCGGT               [AT]^CCGG[AT]
BspHI        3609  TCATGA               T^CATGA
BspLU11I     1026  ACATGT               A^CATGT
BsrBI         721  CCGCTC               CCGCTC(-3/-3)
BssSI        2388  CACGAG               CACGAG(-5/-1)
BstBI         282  TTCGAA               TT^CGAA
BstEII       1124  GGTGACC              G^GT[ACGT]ACC
BstPI        1124  GGTGACC              G^GT[ACGT]ACC
DrdI          921  GACAGAAGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2352  AGCGCT               AGC^GCT
EcoO65I      1124  GGTGACC              G^GT[ACGT]ACC
EcoRV        3548  GATATC               GAT^ATC
FbaI         3180  TGATCA               T^GATCA
FspI         1071  TGCGCA               TGC^GCA
Hin1I        2423  GACGCC               G[GA]^CG[CT]C
HincII       1534  GTCGAC               GT[CT]^[GA]AC
HindII       1534  GTCGAC               GT[CT]^[GA]AC
KpnI         2044  GGTACC               GGTAC^C
NdeI          779  CATATG               CA^TATG
NheI          991  GCTAGC               G^CTAGC
NspV          282  TTCGAA               TT^CGAA
PaeR7I       1146  CTCGAG               C^TCGAG
PflFI        4049  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1279  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        4244  AGGTCCT              [GA]G^G[AT]CC[CT]
RcaI         3609  TCATGA               T^CATGA
SalI         1534  GTCGAC               G^TCGAC
SfuI          282  TTCGAA               TT^CGAA
SmaI          528  CCCGGG               CCC^GGG
SphI         2073  GCATGC               GCATG^C
Tth111I      4049  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1279  CCAGGCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         1146  CTCGAG               C^TCGAG
XmaCI         528  CCCGGG               C^CCGGG
XmaI          528  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccII          43  CGCG                 CG^CG
             3857  CGCG                
Asp700I      1735  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3493  GAATTTCTTC          
AvrII        2786  CCTAGG               C^CTAGG
             4248  CCTAGG              
BanI         2044  GGTACC               G^G[CT][GA]CC
             2332  GGTGCC              
BglI         2756  GCCACTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3960  GCCAGGGAGGC         
BlnI         2786  CCTAGG               C^CTAGG
             4248  CCTAGG              
BsmI          415  GAATGC               GAATGC(1/-1)
             1032  GCATTC              
BstUI          43  CGCG                 CG^CG
             3857  CGCG                
BstXI        1045  CCATCTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3924  CCAGAGGCATGG        
EcoRI          92  GAATTC               G^AATTC
              320  GAATTC              
HaeII         404  AGCGCC               [GA]GCGC^[CT]
             2352  AGCGCT              
MaeII         150  ACGT                 A^CGT
             4072  ACGT                
MvnI           43  CGCG                 CG^CG
             3857  CGCG                
NspI         1026  ACATGT               [GA]CATG^[CT]
             2073  GCATGC              
PshAI         611  GACATCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4239  GACACAGGTC          
SapI          886  GAAGAGC              GCTCTTC(1/4)
             2840  GCTCTTC             
XmnI         1735  GAACAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3493  GAATTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AspHI       
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MfeI        MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII