##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Alw44I ApaLI AscI AvrII
BamHI BclI BlnI BsiWI Bsp1407I
BspHI BsrGI BssSI Bst1107I BstBI
BstZ17I CpoI DrdI EcoRV FbaI
FseI HindIII KspI MfeI MluI
MunI NaeI NgoMIV NheI NruI
NspV PaeR7I PmeI PshAI RcaI
RsrII SacII SalI ScaI SexAI
SfuI SgrAI SnaBI SpeI SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3567 GTAGAC GT^[AC][GT]AC
AclI 4622 AACGTT AA^CGTT
AflII 4375 CTTAAG C^TTAAG
AgeI 1392 ACCGGT A^CCGGT
AhdI 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 986 AGCGCT AGC^GCT
ApaI 2423 GGGCCC GGGCC^C
AseI 2782 ATTAAT AT^TAAT
AsnI 2782 ATTAAT AT^TAAT
Asp700I 4753 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 699 CACGTG CAC^GTG
BbvCI 772 GCTGAGG CCTCAGC(-5/-2)
BcgI 4106 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4375 CTTAAG C^TTAAG
BglII 4188 AGATCT A^GATCT
BsaAI 699 CACGTG [CT]AC^GT[GA]
BsaBI 1464 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3594 CGTCTC CGTCTC(1/5)
BspDI 797 ATCGAT AT^CGAT
BspLU11I 2655 ACATGT A^CATGT
BssHII 525 GCGCGC G^CGCGC
BstEII 3795 GGTAACC G^GT[ACGT]ACC
BstPI 3795 GGTAACC G^GT[ACGT]ACC
ClaI 797 ATCGAT AT^CGAT
EagI 6 CGGCCG C^GGCCG
Eam1105I 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 6 CGGCCG C^GGCCG
Eco47III 986 AGCGCT AGC^GCT
Eco52I 6 CGGCCG C^GGCCG
EcoO65I 3795 GGTAACC G^GT[ACGT]ACC
HpaI 3430 GTTAAC GTT^AAC
MamI 1464 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 5 GCGGCCGC GC^GGCCGC
PacI 2779 TTAATTAA TTAAT^TAA
PflMI 1678 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1392 ACCGGT A^CCGGT
PmaCI 699 CACGTG CAC^GTG
PmlI 699 CACGTG CAC^GTG
PshBI 2782 ATTAAT AT^TAAT
Psp1406I 4622 AACGTT AA^CGTT
PvuI 2707 CGATCG CGAT^CG
SacI 4075 GAGCTC GAGCT^C
SapI 1174 GAAGAGC GCTCTTC(1/4)
SbfI 1766 CCTGCAGG CCTGCA^GG
SfiI 1414 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1766 CCTGCAGG CCTGCA^GG
SspI 2943 AATATT AAT^ATT
Van91I 1678 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3416 CCAGTAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4753 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 707 GGTACC G^GTACC
1395 GGTACC
AccIII 294 TCCGGA T^CCGGA
2670 TCCGGA
AflIII 698 ACACGT A^C[GA][CT]GT
2655 ACATGT
AocI 2513 CCTGAGG CC^T[ACGT]AGG
2642 CCTCAGG
Asp718I 707 GGTACC G^GTACC
1395 GGTACC
BbeI 1628 GGCGCC GGCGC^C
1995 GGCGCC
BciVI 1969 GGATAC GTATCC(6/5)
2508 GTATCC
BsaI 255 GAGACC GGTCTC(1/5)
1789 GAGACC
BseAI 294 TCCGGA T^CCGGA
2670 TCCGGA
BsiEI 6 CGGCCG CG[GA][CT]^CG
2707 CGATCG
BsmI 518 GAATGC GAATGC(1/-1)
3506 GCATTC
BspEI 294 TCCGGA T^CCGGA
2670 TCCGGA
BsrDI 4276 CATTGC GCAATG(2/0)
3952 GCAATG
BstXI 852 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3725 CCAGCAGGCTGG
Bsu36I 2513 CCTGAGG CC^T[ACGT]AGG
2642 CCTCAGG
Eco81I 2513 CCTGAGG CC^T[ACGT]AGG
2642 CCTCAGG
EcoNI 1018 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4562 CCTTTGAAAGG
EcoRI 2087 GAATTC G^AATTC
3685 GAATTC
EcoT22I 3161 ATGCAT ATGCA^T
4474 ATGCAT
KasI 1628 GGCGCC G^GCGCC
1995 GGCGCC
KpnI 707 GGTACC GGTAC^C
1395 GGTACC
MroI 294 TCCGGA T^CCGGA
2670 TCCGGA
NarI 1628 GGCGCC GG^CGCC
1995 GGCGCC
NdeI 2905 CATATG CA^TATG
4484 CATATG
NsiI 3161 ATGCAT ATGCA^T
4474 ATGCAT
SfoI 1628 GGCGCC GGC^GCC
1995 GGCGCC
SphI 744 GCATGC GCATG^C
3550 GCATGC
StuI 535 AGGCCT AGG^CCT
1823 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII XmaCI
XmaI