################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AatII Alw44I ApaLI AscI AvrII BamHI BclI BlnI BsiWI Bsp1407I BspHI BsrGI BssSI Bst1107I BstBI BstZ17I CpoI DrdI EcoRV FbaI FseI HindIII KspI MfeI MluI MunI NaeI NgoMIV NheI NruI NspV PaeR7I PmeI PshAI RcaI RsrII SacII SalI ScaI SexAI SfuI SgrAI SnaBI SpeI SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 3567 GTAGAC GT^[AC][GT]AC AclI 4622 AACGTT AA^CGTT AflII 4375 CTTAAG C^TTAAG AgeI 1392 ACCGGT A^CCGGT AhdI 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 986 AGCGCT AGC^GCT ApaI 2423 GGGCCC GGGCC^C AseI 2782 ATTAAT AT^TAAT AsnI 2782 ATTAAT AT^TAAT Asp700I 4753 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 699 CACGTG CAC^GTG BbvCI 772 GCTGAGG CCTCAGC(-5/-2) BcgI 4106 GCAAGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4375 CTTAAG C^TTAAG BglII 4188 AGATCT A^GATCT BsaAI 699 CACGTG [CT]AC^GT[GA] BsaBI 1464 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 3594 CGTCTC CGTCTC(1/5) BspDI 797 ATCGAT AT^CGAT BspLU11I 2655 ACATGT A^CATGT BssHII 525 GCGCGC G^CGCGC BstEII 3795 GGTAACC G^GT[ACGT]ACC BstPI 3795 GGTAACC G^GT[ACGT]ACC ClaI 797 ATCGAT AT^CGAT EagI 6 CGGCCG C^GGCCG Eam1105I 1346 GACTCCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 6 CGGCCG C^GGCCG Eco47III 986 AGCGCT AGC^GCT Eco52I 6 CGGCCG C^GGCCG EcoO65I 3795 GGTAACC G^GT[ACGT]ACC HpaI 3430 GTTAAC GTT^AAC MamI 1464 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NotI 5 GCGGCCGC GC^GGCCGC PacI 2779 TTAATTAA TTAAT^TAA PflMI 1678 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1392 ACCGGT A^CCGGT PmaCI 699 CACGTG CAC^GTG PmlI 699 CACGTG CAC^GTG PshBI 2782 ATTAAT AT^TAAT Psp1406I 4622 AACGTT AA^CGTT PvuI 2707 CGATCG CGAT^CG SacI 4075 GAGCTC GAGCT^C SapI 1174 GAAGAGC GCTCTTC(1/4) SbfI 1766 CCTGCAGG CCTGCA^GG SfiI 1414 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1766 CCTGCAGG CCTGCA^GG SspI 2943 AATATT AAT^ATT Van91I 1678 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 3416 CCAGTAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 4753 GAACCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 707 GGTACC G^GTACC 1395 GGTACC AccIII 294 TCCGGA T^CCGGA 2670 TCCGGA AflIII 698 ACACGT A^C[GA][CT]GT 2655 ACATGT AocI 2513 CCTGAGG CC^T[ACGT]AGG 2642 CCTCAGG Asp718I 707 GGTACC G^GTACC 1395 GGTACC BbeI 1628 GGCGCC GGCGC^C 1995 GGCGCC BciVI 1969 GGATAC GTATCC(6/5) 2508 GTATCC BsaI 255 GAGACC GGTCTC(1/5) 1789 GAGACC BseAI 294 TCCGGA T^CCGGA 2670 TCCGGA BsiEI 6 CGGCCG CG[GA][CT]^CG 2707 CGATCG BsmI 518 GAATGC GAATGC(1/-1) 3506 GCATTC BspEI 294 TCCGGA T^CCGGA 2670 TCCGGA BsrDI 4276 CATTGC GCAATG(2/0) 3952 GCAATG BstXI 852 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3725 CCAGCAGGCTGG Bsu36I 2513 CCTGAGG CC^T[ACGT]AGG 2642 CCTCAGG Eco81I 2513 CCTGAGG CC^T[ACGT]AGG 2642 CCTCAGG EcoNI 1018 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4562 CCTTTGAAAGG EcoRI 2087 GAATTC G^AATTC 3685 GAATTC EcoT22I 3161 ATGCAT ATGCA^T 4474 ATGCAT KasI 1628 GGCGCC G^GCGCC 1995 GGCGCC KpnI 707 GGTACC GGTAC^C 1395 GGTACC MroI 294 TCCGGA T^CCGGA 2670 TCCGGA NarI 1628 GGCGCC GG^CGCC 1995 GGCGCC NdeI 2905 CATATG CA^TATG 4484 CATATG NsiI 3161 ATGCAT ATGCA^T 4474 ATGCAT SfoI 1628 GGCGCC GGC^GCC 1995 GGCGCC SphI 744 GCATGC GCATG^C 3550 GCATGC StuI 535 AGGCCT AGG^CCT 1823 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII XmaCI XmaI