##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AgeI        AhdI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BsiEI       BsiWI       
BsmBI       BspDI       BsrBI       BsrDI       BssHII      
BstBI       BstUI       CelII       CfoI        ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRV       FauI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     KasI        KpnI        KspI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccIII       3071  TCCGGA               T^CCGGA
Alw44I       4522  GTGCAC               G^TGCAC
AocI         3442  CCTTAGG              CC^T[ACGT]AGG
ApaLI        4522  GTGCAC               G^TGCAC
AspI         3900  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1346  CTCGGG               C^[CT]CG[GA]G
BamHI         782  GGATCC               G^GATCC
BclI         3212  TGATCA               T^GATCA
BglII        2471  AGATCT               A^GATCT
BsaAI        2945  TACGTA               [CT]AC^GT[GA]
BsaBI         201  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3071  TCCGGA               [AT]^CCGG[AT]
BseAI        3071  TCCGGA               T^CCGGA
BsoBI        1346  CTCGGG               C^[CT]CG[GA]G
BspEI        3071  TCCGGA               T^CCGGA
BspHI        2713  TCATGA               T^CATGA
BsrFI        1340  GCCGGT               [GA]^CCGG[CT]
BssSI         722  CTCGTG               CACGAG(-5/-1)
Bst1107I     3785  GTATAC               GTA^TAC
BstAPI        748  GCACAGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3897  GGTGACC              G^GT[ACGT]ACC
BstPI        3897  GGTGACC              G^GT[ACGT]ACC
BstXI        4117  CCATTTATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3785  GTATAC               GTA^TAC
Bsu36I       3442  CCTTAGG              CC^T[ACGT]AGG
Cfr10I       1340  GCCGGT               [GA]^CCGG[CT]
DraIII       4240  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4759  GACAGCCTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3442  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      3897  GGTGACC              G^GT[ACGT]ACC
EcoRI        3015  GAATTC               G^AATTC
EcoT22I      1871  ATGCAT               ATGCA^T
FbaI         3212  TGATCA               T^GATCA
HincII       1660  GTTAAC               GT[CT]^[GA]AC
HindII       1660  GTTAAC               GT[CT]^[GA]AC
HpaI         1660  GTTAAC               GTT^AAC
MamI          201  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3071  TCCGGA               T^CCGGA
NsiI         1871  ATGCAT               ATGCA^T
PflFI        3900  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4538  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         2019  CTGCAG               CTGCA^G
RcaI         2713  TCATGA               T^CATGA
SexAI        1484  ACCTGGT              A^CC[AT]GGT
SnaBI        2945  TACGTA               TAC^GTA
SpeI         3146  ACTAGT               A^CTAGT
SphI          691  GCATGC               GCATG^C
StuI         1116  AGGCCT               AGG^CCT
Tth111I      3900  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4538  CCAGCACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI         1802  CCGC                 CCGC(-3/-1)
             3790  CCGC                
AflII         944  CTTAAG               C^TTAAG
             3796  CTTAAG              
AflIII       1787  ACATGT               A^C[GA][CT]GT
             2076  ACATGT              
AlwNI         178  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              788  CAGCAGCTG           
BalI         1280  TGGCCA               TGG^CCA
             3421  TGGCCA              
BcgI          482  CGACTGCACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              821  GCAGTTGTATCG        
BciVI         855  GGATAC               GTATCC(6/5)
             3160  GGATAC              
BcnI            1  CCCGG                CC^[GC]GG
             4509  CCGGG               
BfrI          944  CTTAAG               C^TTAAG
             3796  CTTAAG              
BseRI         114  GAGGAG               GAGGAG(10/8)
              360  GAGGAG              
BsgI          485  CTGCAC               GTGCAG(16/14)
              490  CTGCAC              
BsmFI        2029  GTCCC                GGGAC(10/14)
             2196  GTCCC               
Bsp1407I     1398  TGTACA               T^GTACA
             2645  TGTACA              
BspLU11I     1787  ACATGT               A^CATGT
             2076  ACATGT              
BsrGI        1398  TGTACA               T^GTACA
             2645  TGTACA              
EaeI         1280  TGGCCA               [CT]^GGCC[GA]
             3421  TGGCCA              
MluNI        1280  TGGCCA               TGG^CCA
             3421  TGGCCA              
MscI         1280  TGGCCA               TGG^CCA
             3421  TGGCCA              
NciI            1  CCCGG                CC^[GC]GG
             4509  CCGGG               
NcoI          108  CCATGG               C^CATGG
              700  CCATGG              
NdeI         2572  CATATG               CA^TATG
             3476  CATATG              
PpuMI         118  AGGACCT              [GA]G^G[AT]CC[CT]
             2194  GGGTCCC             
SspBI        1398  TGTACA               T^GTACA
             2645  TGTACA              
XcmI         1085  CCAGATAAGTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4441  CCACTGTAGAAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     AspHI       
AvaII       BanII       BbsI        BbvI        BfaI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BspMI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XbaI        XhoII       XmnI