##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AviII AvrII BanI
BbeI BbrPI BbvCI BglI BlnI
BlpI Bpu1102I BsaHI BsiEI BsiWI
BsmBI BspDI BsrBI BsrDI BssHII
BstBI BstUI CelII CfoI ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 3071 TCCGGA T^CCGGA
Alw44I 4522 GTGCAC G^TGCAC
AocI 3442 CCTTAGG CC^T[ACGT]AGG
ApaLI 4522 GTGCAC G^TGCAC
AspI 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1346 CTCGGG C^[CT]CG[GA]G
BamHI 782 GGATCC G^GATCC
BclI 3212 TGATCA T^GATCA
BglII 2471 AGATCT A^GATCT
BsaAI 2945 TACGTA [CT]AC^GT[GA]
BsaBI 201 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3071 TCCGGA [AT]^CCGG[AT]
BseAI 3071 TCCGGA T^CCGGA
BsoBI 1346 CTCGGG C^[CT]CG[GA]G
BspEI 3071 TCCGGA T^CCGGA
BspHI 2713 TCATGA T^CATGA
BsrFI 1340 GCCGGT [GA]^CCGG[CT]
BssSI 722 CTCGTG CACGAG(-5/-1)
Bst1107I 3785 GTATAC GTA^TAC
BstAPI 748 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3897 GGTGACC G^GT[ACGT]ACC
BstPI 3897 GGTGACC G^GT[ACGT]ACC
BstXI 4117 CCATTTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3785 GTATAC GTA^TAC
Bsu36I 3442 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1340 GCCGGT [GA]^CCGG[CT]
DraIII 4240 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4759 GACAGCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3442 CCTTAGG CC^T[ACGT]AGG
EcoO65I 3897 GGTGACC G^GT[ACGT]ACC
EcoRI 3015 GAATTC G^AATTC
EcoT22I 1871 ATGCAT ATGCA^T
FbaI 3212 TGATCA T^GATCA
HincII 1660 GTTAAC GT[CT]^[GA]AC
HindII 1660 GTTAAC GT[CT]^[GA]AC
HpaI 1660 GTTAAC GTT^AAC
MamI 201 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3071 TCCGGA T^CCGGA
NsiI 1871 ATGCAT ATGCA^T
PflFI 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4538 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2019 CTGCAG CTGCA^G
RcaI 2713 TCATGA T^CATGA
SexAI 1484 ACCTGGT A^CC[AT]GGT
SnaBI 2945 TACGTA TAC^GTA
SpeI 3146 ACTAGT A^CTAGT
SphI 691 GCATGC GCATG^C
StuI 1116 AGGCCT AGG^CCT
Tth111I 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4538 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 1802 CCGC CCGC(-3/-1)
3790 CCGC
AflII 944 CTTAAG C^TTAAG
3796 CTTAAG
AflIII 1787 ACATGT A^C[GA][CT]GT
2076 ACATGT
AlwNI 178 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
788 CAGCAGCTG
BalI 1280 TGGCCA TGG^CCA
3421 TGGCCA
BcgI 482 CGACTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
821 GCAGTTGTATCG
BciVI 855 GGATAC GTATCC(6/5)
3160 GGATAC
BcnI 1 CCCGG CC^[GC]GG
4509 CCGGG
BfrI 944 CTTAAG C^TTAAG
3796 CTTAAG
BseRI 114 GAGGAG GAGGAG(10/8)
360 GAGGAG
BsgI 485 CTGCAC GTGCAG(16/14)
490 CTGCAC
BsmFI 2029 GTCCC GGGAC(10/14)
2196 GTCCC
Bsp1407I 1398 TGTACA T^GTACA
2645 TGTACA
BspLU11I 1787 ACATGT A^CATGT
2076 ACATGT
BsrGI 1398 TGTACA T^GTACA
2645 TGTACA
EaeI 1280 TGGCCA [CT]^GGCC[GA]
3421 TGGCCA
MluNI 1280 TGGCCA TGG^CCA
3421 TGGCCA
MscI 1280 TGGCCA TGG^CCA
3421 TGGCCA
NciI 1 CCCGG CC^[GC]GG
4509 CCGGG
NcoI 108 CCATGG C^CATGG
700 CCATGG
NdeI 2572 CATATG CA^TATG
3476 CATATG
PpuMI 118 AGGACCT [GA]G^G[AT]CC[CT]
2194 GGGTCCC
SspBI 1398 TGTACA T^GTACA
2645 TGTACA
XcmI 1085 CCAGATAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4441 CCACTGTAGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI XhoII XmnI