################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BanI BbeI BbrPI BbvCI BglI BlnI BlpI Bpu1102I BsaHI BsiEI BsiWI BsmBI BspDI BsrBI BsrDI BssHII BstBI BstUI CelII CfoI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 3071 TCCGGA T^CCGGA Alw44I 4522 GTGCAC G^TGCAC AocI 3442 CCTTAGG CC^T[ACGT]AGG ApaLI 4522 GTGCAC G^TGCAC AspI 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1346 CTCGGG C^[CT]CG[GA]G BamHI 782 GGATCC G^GATCC BclI 3212 TGATCA T^GATCA BglII 2471 AGATCT A^GATCT BsaAI 2945 TACGTA [CT]AC^GT[GA] BsaBI 201 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3071 TCCGGA [AT]^CCGG[AT] BseAI 3071 TCCGGA T^CCGGA BsoBI 1346 CTCGGG C^[CT]CG[GA]G BspEI 3071 TCCGGA T^CCGGA BspHI 2713 TCATGA T^CATGA BsrFI 1340 GCCGGT [GA]^CCGG[CT] BssSI 722 CTCGTG CACGAG(-5/-1) Bst1107I 3785 GTATAC GTA^TAC BstAPI 748 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 3897 GGTGACC G^GT[ACGT]ACC BstPI 3897 GGTGACC G^GT[ACGT]ACC BstXI 4117 CCATTTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3785 GTATAC GTA^TAC Bsu36I 3442 CCTTAGG CC^T[ACGT]AGG Cfr10I 1340 GCCGGT [GA]^CCGG[CT] DraIII 4240 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4759 GACAGCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3442 CCTTAGG CC^T[ACGT]AGG EcoO65I 3897 GGTGACC G^GT[ACGT]ACC EcoRI 3015 GAATTC G^AATTC EcoT22I 1871 ATGCAT ATGCA^T FbaI 3212 TGATCA T^GATCA HincII 1660 GTTAAC GT[CT]^[GA]AC HindII 1660 GTTAAC GT[CT]^[GA]AC HpaI 1660 GTTAAC GTT^AAC MamI 201 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3071 TCCGGA T^CCGGA NsiI 1871 ATGCAT ATGCA^T PflFI 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4538 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 2019 CTGCAG CTGCA^G RcaI 2713 TCATGA T^CATGA SexAI 1484 ACCTGGT A^CC[AT]GGT SnaBI 2945 TACGTA TAC^GTA SpeI 3146 ACTAGT A^CTAGT SphI 691 GCATGC GCATG^C StuI 1116 AGGCCT AGG^CCT Tth111I 3900 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4538 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 1802 CCGC CCGC(-3/-1) 3790 CCGC AflII 944 CTTAAG C^TTAAG 3796 CTTAAG AflIII 1787 ACATGT A^C[GA][CT]GT 2076 ACATGT AlwNI 178 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 788 CAGCAGCTG BalI 1280 TGGCCA TGG^CCA 3421 TGGCCA BcgI 482 CGACTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 821 GCAGTTGTATCG BciVI 855 GGATAC GTATCC(6/5) 3160 GGATAC BcnI 1 CCCGG CC^[GC]GG 4509 CCGGG BfrI 944 CTTAAG C^TTAAG 3796 CTTAAG BseRI 114 GAGGAG GAGGAG(10/8) 360 GAGGAG BsgI 485 CTGCAC GTGCAG(16/14) 490 CTGCAC BsmFI 2029 GTCCC GGGAC(10/14) 2196 GTCCC Bsp1407I 1398 TGTACA T^GTACA 2645 TGTACA BspLU11I 1787 ACATGT A^CATGT 2076 ACATGT BsrGI 1398 TGTACA T^GTACA 2645 TGTACA EaeI 1280 TGGCCA [CT]^GGCC[GA] 3421 TGGCCA MluNI 1280 TGGCCA TGG^CCA 3421 TGGCCA MscI 1280 TGGCCA TGG^CCA 3421 TGGCCA NciI 1 CCCGG CC^[GC]GG 4509 CCGGG NcoI 108 CCATGG C^CATGG 700 CCATGG NdeI 2572 CATATG CA^TATG 3476 CATATG PpuMI 118 AGGACCT [GA]G^G[AT]CC[CT] 2194 GGGTCCC SspBI 1398 TGTACA T^GTACA 2645 TGTACA XcmI 1085 CCAGATAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4441 CCACTGTAGAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI