##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AvrII BamHI
BbrPI BbvCI BcgI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI HgaI KpnI
MamI MluI MroI NaeI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I RcaI RsrII
SacI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 101 GGCGCC G[GA]^CG[CT]C
AflII 3320 CTTAAG C^TTAAG
AflIII 929 ACATGT A^C[GA][CT]GT
AhdI 3974 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3475 GGGCCC GGGCC^C
AspEI 3974 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3194 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3155 CCCGAG C^[CT]CG[GA]G
AviII 1842 TGCGCA TGC^GCA
BanI 101 GGCGCC G^G[CT][GA]CC
BbeI 101 GGCGCC GGCGC^C
BciVI 1353 GTATCC GTATCC(6/5)
BcnI 1707 CCCGG CC^[GC]GG
BfrI 3320 CTTAAG C^TTAAG
BglII 4377 AGATCT A^GATCT
BsaHI 101 GGCGCC G[GA]^CG[CT]C
BsaI 2058 GAGACC GGTCTC(1/5)
BsaWI 2244 ACCGGA [AT]^CCGG[AT]
BsiEI 3833 CGATCG CG[GA][CT]^CG
BsoBI 3155 CCCGAG C^[CT]CG[GA]G
BspLU11I 929 ACATGT A^CATGT
BsrBI 1756 GAGCGG CCGCTC(-3/-3)
BstXI 3567 CCAATGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2497 GACATCCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3974 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1842 TGCGCA TGC^GCA
Hin1I 101 GGCGCC G[GA]^CG[CT]C
KasI 101 GGCGCC G^GCGCC
KspI 105 CCGCGG CCGC^GG
MaeII 345 ACGT A^CGT
NarI 101 GGCGCC GG^CGCC
NciI 1707 CCCGG CC^[GC]GG
NheI 2650 GCTAGC G^CTAGC
PflFI 3194 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1116 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 3833 CGATCG CGAT^CG
SacII 105 CCGCGG CCGC^GG
SexAI 459 ACCTGGT A^CC[AT]GGT
SfoI 101 GGCGCC GGC^GCC
SwaI 4685 ATTTAAAT ATTT^AAAT
Tth111I 3194 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 106 CGCG CG^CG
290 CGCG
Asp700I 223 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3263 GAAGATGTTC
AspHI 185 GTGCTC G[AT]GC[AT]^C
4526 GAGCAC
BsiHKAI 185 GTGCTC G[AT]GC[AT]^C
4526 GAGCAC
BsrDI 1471 CATTGC GCAATG(2/0)
4560 GCAATG
BstUI 106 CGCG CG^CG
290 CGCG
BstYI 1077 AGATCC [GA]^GATC[CT]
4377 AGATCT
DraII 1116 AGGACCC [GA]G^G[ACGT]CC[CT]
3475 GGGCCCC
DraIII 2198 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4314 CACTAAGTG
EcoO109I 1116 AGGACCC [GA]G^G[ACGT]CC[CT]
3475 GGGCCCC
EcoT14I 180 CCATGG C^C[AT][AT]GG
2532 CCATGG
HapII 1708 CCGG C^CGG
2245 CCGG
HindIII 693 AAGCTT A^AGCTT
4694 AAGCTT
HpaII 1708 CCGG C^CGG
2245 CCGG
MflI 1077 AGATCC [GA]^GATC[CT]
4377 AGATCT
MspI 1708 CCGG C^CGG
2245 CCGG
MvnI 106 CGCG CG^CG
290 CGCG
NcoI 180 CCATGG C^CATGG
2532 CCATGG
PflMI 1409 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2519 CCAACTCTTGG
PshAI 1389 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2530 GACCATGGTC
SapI 4555 GAAGAGC GCTCTTC(1/4)
2458 GCTCTTC
SspI 2051 AATATT AAT^ATT
4733 AATATT
StyI 180 CCATGG C^C[AT][AT]GG
2532 CCATGG
Van91I 1409 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2519 CCAACTCTTGG
XbaI 538 TCTAGA T^CTAGA
1501 TCTAGA
XhoII 1077 AGATCC [GA]^GATC[CT]
4377 AGATCT
XmnI 223 GAAGCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3263 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BanII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoNI EcoRI
EcoRII FauI FbaI Fnu4HI FokI
HaeII HaeIII HhaI HinP1I HincII
HindII HinfI HpaI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MfeI MluNI MnlI MscI MseI
MslI MspA1I MunI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SalI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI