################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccI AclI AflII AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII BbeI BbsI BciVI BclI BfrI BpuAI BsaBI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FbaI FspI HpaI KasI KspI MamI MfeI MluI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 478 GGTACC G^GTACC AccIII 757 TCCGGA T^CCGGA AcsI 471 GAATTC [GA]^AATT[CT] AcyI 3753 GGCGTC G[GA]^CG[CT]C AgeI 2317 ACCGGT A^CCGGT ApoI 471 GAATTC [GA]^AATT[CT] Asp718I 478 GGTACC G^GTACC AvrII 3685 CCTAGG C^CTAGG BbrPI 1345 CACGTG CAC^GTG BcgI 2476 CGAGCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3685 CCTAGG C^CTAGG BlpI 3704 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3704 GCTCAGC GC^T[ACGT]AGC BsaAI 1345 CACGTG [CT]AC^GT[GA] BsaHI 3753 GGCGTC G[GA]^CG[CT]C BseAI 757 TCCGGA T^CCGGA BspEI 757 TCCGGA T^CCGGA BspLU11I 2843 ACATGT A^CATGT BsrBI 246 CCGCTC CCGCTC(-3/-3) BstEII 2020 GGTCACC G^GT[ACGT]ACC BstPI 2020 GGTCACC G^GT[ACGT]ACC CelII 3704 GCTCAGC GC^T[ACGT]AGC DraIII 178 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 2020 GGTCACC G^GT[ACGT]ACC EcoRI 471 GAATTC G^AATTC FseI 3464 GGCCGGCC GGCCGG^CC HaeII 499 AGCGCC [GA]GCGC^[CT] Hin1I 3753 GGCGTC G[GA]^CG[CT]C HindIII 2094 AAGCTT A^AGCTT KpnI 478 GGTACC GGTAC^C MroI 757 TCCGGA T^CCGGA NdeI 1596 CATATG CA^TATG PinAI 2317 ACCGGT A^CCGGT PmaCI 1345 CACGTG CAC^GTG PmlI 1345 CACGTG CAC^GTG SacI 824 GAGCTC GAGCT^C SapI 1443 GAAGAGC GCTCTTC(1/4) ScaI 791 AGTACT AGT^ACT SexAI 2332 ACCTGGT A^CC[AT]GGT SspI 3363 AATATT AAT^ATT XbaI 2978 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AflIII 1344 ACACGT A^C[GA][CT]GT 2843 ACATGT ApaI 801 GGGCCC GGGCC^C 1743 GGGCCC Asp700I 45 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2897 GAAGCTGTTC BamHI 856 GGATCC G^GATCC 1959 GGATCC BglII 1302 AGATCT A^GATCT 1773 AGATCT BssSI 462 CACGAG CACGAG(-5/-1) 2474 CACGAG BstAPI 416 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1682 GCATCCACTGC EcoRV 264 GATATC GAT^ATC 1863 GATATC FauI 2644 GCGGG CCCGC(4/6) 3528 GCGGG HincII 1475 GTCAAC GT[CT]^[GA]AC 1875 GTCAAC HindII 1475 GTCAAC GT[CT]^[GA]AC 1875 GTCAAC NaeI 2542 GCCGGC GCC^GGC 3465 GCCGGC NgoMIV 2542 GCCGGC G^CCGGC 3465 GCCGGC NspI 2825 ACATGC [GA]CATG^[CT] 2843 ACATGT SbfI 145 CCTGCAGG CCTGCA^GG 2917 CCTGCAGG Sse8387I 145 CCTGCAGG CCTGCA^GG 2917 CCTGCAGG StuI 1102 AGGCCT AGG^CCT 2380 AGGCCT XmnI 45 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2897 GAAGCTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII