##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccI AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII BbeI
BbsI BciVI BclI BfrI BpuAI
BsaBI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoT22I FbaI FspI HpaI
KasI KspI MamI MfeI MluI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI TaqI Tth111I
TthHB8I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 478 GGTACC G^GTACC
AccIII 757 TCCGGA T^CCGGA
AcsI 471 GAATTC [GA]^AATT[CT]
AcyI 3753 GGCGTC G[GA]^CG[CT]C
AgeI 2317 ACCGGT A^CCGGT
ApoI 471 GAATTC [GA]^AATT[CT]
Asp718I 478 GGTACC G^GTACC
AvrII 3685 CCTAGG C^CTAGG
BbrPI 1345 CACGTG CAC^GTG
BcgI 2476 CGAGCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3685 CCTAGG C^CTAGG
BlpI 3704 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3704 GCTCAGC GC^T[ACGT]AGC
BsaAI 1345 CACGTG [CT]AC^GT[GA]
BsaHI 3753 GGCGTC G[GA]^CG[CT]C
BseAI 757 TCCGGA T^CCGGA
BspEI 757 TCCGGA T^CCGGA
BspLU11I 2843 ACATGT A^CATGT
BsrBI 246 CCGCTC CCGCTC(-3/-3)
BstEII 2020 GGTCACC G^GT[ACGT]ACC
BstPI 2020 GGTCACC G^GT[ACGT]ACC
CelII 3704 GCTCAGC GC^T[ACGT]AGC
DraIII 178 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2020 GGTCACC G^GT[ACGT]ACC
EcoRI 471 GAATTC G^AATTC
FseI 3464 GGCCGGCC GGCCGG^CC
HaeII 499 AGCGCC [GA]GCGC^[CT]
Hin1I 3753 GGCGTC G[GA]^CG[CT]C
HindIII 2094 AAGCTT A^AGCTT
KpnI 478 GGTACC GGTAC^C
MroI 757 TCCGGA T^CCGGA
NdeI 1596 CATATG CA^TATG
PinAI 2317 ACCGGT A^CCGGT
PmaCI 1345 CACGTG CAC^GTG
PmlI 1345 CACGTG CAC^GTG
SacI 824 GAGCTC GAGCT^C
SapI 1443 GAAGAGC GCTCTTC(1/4)
ScaI 791 AGTACT AGT^ACT
SexAI 2332 ACCTGGT A^CC[AT]GGT
SspI 3363 AATATT AAT^ATT
XbaI 2978 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1344 ACACGT A^C[GA][CT]GT
2843 ACATGT
ApaI 801 GGGCCC GGGCC^C
1743 GGGCCC
Asp700I 45 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2897 GAAGCTGTTC
BamHI 856 GGATCC G^GATCC
1959 GGATCC
BglII 1302 AGATCT A^GATCT
1773 AGATCT
BssSI 462 CACGAG CACGAG(-5/-1)
2474 CACGAG
BstAPI 416 GCAAGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1682 GCATCCACTGC
EcoRV 264 GATATC GAT^ATC
1863 GATATC
FauI 2644 GCGGG CCCGC(4/6)
3528 GCGGG
HincII 1475 GTCAAC GT[CT]^[GA]AC
1875 GTCAAC
HindII 1475 GTCAAC GT[CT]^[GA]AC
1875 GTCAAC
NaeI 2542 GCCGGC GCC^GGC
3465 GCCGGC
NgoMIV 2542 GCCGGC G^CCGGC
3465 GCCGGC
NspI 2825 ACATGC [GA]CATG^[CT]
2843 ACATGT
SbfI 145 CCTGCAGG CCTGCA^GG
2917 CCTGCAGG
Sse8387I 145 CCTGCAGG CCTGCA^GG
2917 CCTGCAGG
StuI 1102 AGGCCT AGG^CCT
2380 AGGCCT
XmnI 45 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2897 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII