##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       AccI        AclI        AflII       AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       BbeI        
BbsI        BciVI       BclI        BfrI        BpuAI       
BsaBI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BsrGI       BssHII      
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoT22I     FbaI        FspI        HpaI        
KasI        KspI        MamI        MfeI        MluI        
MunI        NarI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SwaI        TaqI        Tth111I     
TthHB8I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        478  GGTACC               G^GTACC
AccIII        757  TCCGGA               T^CCGGA
AcsI          471  GAATTC               [GA]^AATT[CT]
AcyI         3753  GGCGTC               G[GA]^CG[CT]C
AgeI         2317  ACCGGT               A^CCGGT
ApoI          471  GAATTC               [GA]^AATT[CT]
Asp718I       478  GGTACC               G^GTACC
AvrII        3685  CCTAGG               C^CTAGG
BbrPI        1345  CACGTG               CAC^GTG
BcgI         2476  CGAGCCAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         3685  CCTAGG               C^CTAGG
BlpI         3704  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3704  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1345  CACGTG               [CT]AC^GT[GA]
BsaHI        3753  GGCGTC               G[GA]^CG[CT]C
BseAI         757  TCCGGA               T^CCGGA
BspEI         757  TCCGGA               T^CCGGA
BspLU11I     2843  ACATGT               A^CATGT
BsrBI         246  CCGCTC               CCGCTC(-3/-3)
BstEII       2020  GGTCACC              G^GT[ACGT]ACC
BstPI        2020  GGTCACC              G^GT[ACGT]ACC
CelII        3704  GCTCAGC              GC^T[ACGT]AGC
DraIII        178  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      2020  GGTCACC              G^GT[ACGT]ACC
EcoRI         471  GAATTC               G^AATTC
FseI         3464  GGCCGGCC             GGCCGG^CC
HaeII         499  AGCGCC               [GA]GCGC^[CT]
Hin1I        3753  GGCGTC               G[GA]^CG[CT]C
HindIII      2094  AAGCTT               A^AGCTT
KpnI          478  GGTACC               GGTAC^C
MroI          757  TCCGGA               T^CCGGA
NdeI         1596  CATATG               CA^TATG
PinAI        2317  ACCGGT               A^CCGGT
PmaCI        1345  CACGTG               CAC^GTG
PmlI         1345  CACGTG               CAC^GTG
SacI          824  GAGCTC               GAGCT^C
SapI         1443  GAAGAGC              GCTCTTC(1/4)
ScaI          791  AGTACT               AGT^ACT
SexAI        2332  ACCTGGT              A^CC[AT]GGT
SspI         3363  AATATT               AAT^ATT
XbaI         2978  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII       1344  ACACGT               A^C[GA][CT]GT
             2843  ACATGT              
ApaI          801  GGGCCC               GGGCC^C
             1743  GGGCCC              
Asp700I        45  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2897  GAAGCTGTTC          
BamHI         856  GGATCC               G^GATCC
             1959  GGATCC              
BglII        1302  AGATCT               A^GATCT
             1773  AGATCT              
BssSI         462  CACGAG               CACGAG(-5/-1)
             2474  CACGAG              
BstAPI        416  GCAAGAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1682  GCATCCACTGC         
EcoRV         264  GATATC               GAT^ATC
             1863  GATATC              
FauI         2644  GCGGG                CCCGC(4/6)
             3528  GCGGG               
HincII       1475  GTCAAC               GT[CT]^[GA]AC
             1875  GTCAAC              
HindII       1475  GTCAAC               GT[CT]^[GA]AC
             1875  GTCAAC              
NaeI         2542  GCCGGC               GCC^GGC
             3465  GCCGGC              
NgoMIV       2542  GCCGGC               G^CCGGC
             3465  GCCGGC              
NspI         2825  ACATGC               [GA]CATG^[CT]
             2843  ACATGT              
SbfI          145  CCTGCAGG             CCTGCA^GG
             2917  CCTGCAGG            
Sse8387I      145  CCTGCAGG             CCTGCA^GG
             2917  CCTGCAGG            
StuI         1102  AGGCCT               AGG^CCT
             2380  AGGCCT              
XmnI           45  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2897  GAAGCTGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AocI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvCI       BbvI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PshAI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Van91I      XcmI        XhoII