##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AvrII BamHI BbeI
BbrPI BglI BlnI BsaWI BseAI
BsiEI BspDI BspEI BspHI BstAPI
BstEII BstPI Bsu36I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI KasI
KpnI KspI MfeI MroI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI Sse8387I StuI SwaI XbaI
XcmI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 1986 CTTAAG C^TTAAG
ApaI 158 GGGCCC GGGCC^C
AspHI 150 GAGCTC G[AT]GC[AT]^C
BanI 4479 GGTGCC G^G[CT][GA]CC
BcgI 3893 CGACATCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3095 GGATAC GTATCC(6/5)
BclI 3797 TGATCA T^GATCA
BfrI 1986 CTTAAG C^TTAAG
BlpI 3394 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3394 GCTCAGC GC^T[ACGT]AGC
BsaBI 1431 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4342 GGTCTC GGTCTC(1/5)
BsiHKAI 150 GAGCTC G[AT]GC[AT]^C
BsiWI 3949 CGTACG C^GTACG
BspLU11I 2780 ACATGT A^CATGT
BspMI 3297 GCAGGT ACCTGC(4/8)
BsrBI 2807 CCGCTC CCGCTC(-3/-3)
BssSI 3694 CTCGTG CACGAG(-5/-1)
Bst1107I 320 GTATAC GTA^TAC
BstBI 2526 TTCGAA TT^CGAA
BstXI 736 CCAAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 320 GTATAC GTA^TAC
CelII 3394 GCTCAGC GC^T[ACGT]AGC
DraIII 1736 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1982 GACTCTTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2692 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 747 GAATTC G^AATTC
FbaI 3797 TGATCA T^GATCA
HindIII 566 AAGCTT A^AGCTT
HpaI 3081 GTTAAC GTT^AAC
MamI 1431 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2883 ACGCGT A^CGCGT
NdeI 1065 CATATG CA^TATG
NspV 2526 TTCGAA TT^CGAA
PflMI 4264 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 391 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 150 GAGCTC GAGCT^C
SexAI 1952 ACCTGGT A^CC[AT]GGT
SfuI 2526 TTCGAA TT^CGAA
SpeI 890 ACTAGT A^CTAGT
SphI 2687 GCATGC GCATG^C
Van91I 4264 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1419 GACGTC GACGT^C
2074 GACGTC
AseI 2619 ATTAAT AT^TAAT
3466 ATTAAT
AsnI 2619 ATTAAT AT^TAAT
3466 ATTAAT
AviII 203 TGCGCA TGC^GCA
2925 TGCGCA
BalI 3364 TGGCCA TGG^CCA
3915 TGGCCA
BbvCI 992 GCTGAGG CCTCAGC(-5/-2)
1290 GCTGAGG
BglII 2187 AGATCT A^GATCT
2410 AGATCT
BsaAI 1022 CACGTA [CT]AC^GT[GA]
3951 TACGTG
BsgI 165 CTGCAC GTGCAG(16/14)
1566 GTGCAG
BsmBI 1455 GAGACG CGTCTC(1/5)
2488 GAGACG
Bsp1407I 2818 TGTACA T^GTACA
4154 TGTACA
BsrGI 2818 TGTACA T^GTACA
4154 TGTACA
DraII 158 GGGCCCT [GA]G^G[ACGT]CC[CT]
391 AGGTCCT
EaeI 3364 TGGCCA [CT]^GGCC[GA]
3915 TGGCCA
EarI 117 GAAGAG CTCTTC(1/4)
1611 GAAGAG
EcoO109I 158 GGGCCCT [GA]G^G[ACGT]CC[CT]
391 AGGTCCT
FspI 203 TGCGCA TGC^GCA
2925 TGCGCA
HaeII 3045 AGCGCC [GA]GCGC^[CT]
3194 AGCGCC
HincII 3081 GTTAAC GT[CT]^[GA]AC
3706 GTTGAC
HindII 3081 GTTAAC GT[CT]^[GA]AC
3706 GTTGAC
Ksp632I 117 GAAGAG CTCTTC(1/4)
1611 GAAGAG
MluNI 3364 TGGCCA TGG^CCA
3915 TGGCCA
MscI 3364 TGGCCA TGG^CCA
3915 TGGCCA
PaeR7I 794 CTCGAG C^TCGAG
2965 CTCGAG
PshBI 2619 ATTAAT AT^TAAT
3466 ATTAAT
SmaI 40 CCCGGG CCC^GGG
2800 CCCGGG
SspBI 2818 TGTACA T^GTACA
4154 TGTACA
SspI 2558 AATATT AAT^ATT
4046 AATATT
TfiI 2065 GAATC G^A[AT]TC
3136 GAATC
XhoI 794 CTCGAG C^TCGAG
2965 CTCGAG
XmaCI 40 CCCGGG C^CCGGG
2800 CCCGGG
XmaI 40 CCCGGG C^CCGGG
2800 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DsaI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflFI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XhoII XmnI