##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AspEI AviII AvrII
BbeI BbvCI BglII BlnI BlpI
Bpu1102I BsiEI BsiWI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII KasI KspI MluI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PmeI PpuMI PshAI
PvuI RcaI RsrII SacI SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I StuI SwaI XbaI XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 3549 GACGTC GACGT^C
Acc65I 2745 GGTACC G^GTACC
AclI 4010 AACGTT AA^CGTT
AgeI 4524 ACCGGT A^CCGGT
ApaI 2834 GGGCCC GGGCC^C
Asp718I 2745 GGTACC G^GTACC
AspHI 2975 GTGCTC G[AT]GC[AT]^C
AspI 170 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1094 TGGCCA TGG^CCA
BbrPI 1387 CACGTG CAC^GTG
BpmI 2796 CTCCAG CTGGAG(16/14)
BsaAI 1387 CACGTG [CT]AC^GT[GA]
BsaI 2793 GGTCTC GGTCTC(1/5)
BsiHKAI 2975 GTGCTC G[AT]GC[AT]^C
BsmI 1144 GCATTC GAATGC(1/-1)
BspDI 795 ATCGAT AT^CGAT
BsrBI 49 CCGCTC CCGCTC(-3/-3)
BsrDI 581 GCAATG GCAATG(2/0)
BsrFI 4524 ACCGGT [GA]^CCGG[CT]
BssSI 2089 CACGAG CACGAG(-5/-1)
BstAPI 1159 GCAGACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 4524 ACCGGT [GA]^CCGG[CT]
ClaI 795 ATCGAT AT^CGAT
DraII 3024 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1094 TGGCCA [CT]^GGCC[GA]
EcoNI 3031 CCTCAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3024 AGGCCCT [GA]G^G[ACGT]CC[CT]
HpaI 3268 GTTAAC GTT^AAC
KpnI 2745 GGTACC GGTAC^C
MluNI 1094 TGGCCA TGG^CCA
MscI 1094 TGGCCA TGG^CCA
NheI 780 GCTAGC G^CTAGC
PaeR7I 258 CTCGAG C^TCGAG
PflFI 170 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4524 ACCGGT A^CCGGT
PmaCI 1387 CACGTG CAC^GTG
PmlI 1387 CACGTG CAC^GTG
Psp1406I 4010 AACGTT AA^CGTT
SalI 1902 GTCGAC G^TCGAC
SapI 1270 GAAGAGC GCTCTTC(1/4)
SphI 3185 GCATGC GCATG^C
Tth111I 170 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 258 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 790 TCCGGA T^CCGGA
1511 TCCGGA
AcyI 1656 GGCGTC G[GA]^CG[CT]C
3549 GACGTC
AflII 4242 CTTAAG C^TTAAG
4326 CTTAAG
Asp700I 2248 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3036 GAAAGGTTTC
AvaI 258 CTCGAG C^[CT]CG[GA]G
330 CCCGAG
BamHI 471 GGATCC G^GATCC
3803 GGATCC
BanII 1751 GGGCTC G[GA]GC[CT]^C
2834 GGGCCC
BcgI 797 CGATAACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1958 GCAGTCTACTCG
BciVI 985 GGATAC GTATCC(6/5)
1366 GGATAC
BclI 866 TGATCA T^GATCA
3131 TGATCA
BcnI 375 CCCGG CC^[GC]GG
1936 CCCGG
BfrI 4242 CTTAAG C^TTAAG
4326 CTTAAG
BglI 256 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4489 GCCTCTGTGGC
BsaHI 1656 GGCGTC G[GA]^CG[CT]C
3549 GACGTC
BseAI 790 TCCGGA T^CCGGA
1511 TCCGGA
BsmBI 237 GAGACG CGTCTC(1/5)
1293 GAGACG
BsoBI 258 CTCGAG C^[CT]CG[GA]G
330 CCCGAG
Bsp1407I 4092 TGTACA T^GTACA
4225 TGTACA
BspEI 790 TCCGGA T^CCGGA
1511 TCCGGA
BsrGI 4092 TGTACA T^GTACA
4225 TGTACA
BstXI 2166 CCACATGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2647 CCAAATAATTGG
FbaI 866 TGATCA T^GATCA
3131 TGATCA
Hin1I 1656 GGCGTC G[GA]^CG[CT]C
3549 GACGTC
HincII 1902 GTCGAC GT[CT]^[GA]AC
3268 GTTAAC
HindII 1902 GTCGAC GT[CT]^[GA]AC
3268 GTTAAC
MfeI 2759 CAATTG C^AATTG
3391 CAATTG
MroI 790 TCCGGA T^CCGGA
1511 TCCGGA
MunI 2759 CAATTG C^AATTG
3391 CAATTG
NciI 375 CCCGG CC^[GC]GG
1936 CCCGG
NdeI 3749 CATATG CA^TATG
4107 CATATG
PstI 1838 CTGCAG CTGCA^G
3020 CTGCAG
PvuII 1554 CAGCTG CAG^CTG
4464 CAGCTG
SspBI 4092 TGTACA T^GTACA
4225 TGTACA
SspI 2438 AATATT AAT^ATT
4076 AATATT
XmnI 2248 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3036 GAAAGGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AseI
AsnI AvaII BanI BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DrdI DsaI EarI
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII