##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccII AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII BamHI BbeI
BbrPI BcgI BfrI BglII BlpI
Bpu1102I BseAI BsiEI BsmBI BspDI
BspEI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI HinP1I HpaI KasI
KpnI KspI MluI MroI MvnI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfoI SfuI SgrAI SnaBI
SspI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2840 GACGTC GACGT^C
AcyI 2840 GACGTC G[GA]^CG[CT]C
AflIII 1749 ACACGT A^C[GA][CT]GT
AhdI 3410 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3410 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1141 CCTAGG C^CTAGG
BalI 1612 TGGCCA TGG^CCA
BbvCI 3173 CCTCAGC CCTCAGC(-5/-2)
BlnI 1141 CCTAGG C^CTAGG
BsaAI 1750 CACGTA [CT]AC^GT[GA]
BsaBI 1994 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2840 GACGTC G[GA]^CG[CT]C
BsaWI 1596 ACCGGA [AT]^CCGG[AT]
BsgI 1835 GTGCAG GTGCAG(16/14)
BsiWI 1752 CGTACG C^GTACG
BsmI 984 GCATTC GAATGC(1/-1)
Bsp1407I 1722 TGTACA T^GTACA
BsrGI 1722 TGTACA T^GTACA
BstYI 2512 AGATCC [GA]^GATC[CT]
DraI 3760 TTTAAA TTT^AAA
Eam1105I 3410 GACTCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Hin1I 2840 GACGTC G[GA]^CG[CT]C
MamI 1994 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3892 CAATTG C^AATTG
MflI 2512 AGATCC [GA]^GATC[CT]
MluNI 1612 TGGCCA TGG^CCA
MscI 1612 TGGCCA TGG^CCA
MunI 3892 CAATTG C^AATTG
NcoI 1060 CCATGG C^CATGG
SacI 2443 GAGCTC GAGCT^C
SbfI 3063 CCTGCAGG CCTGCA^GG
ScaI 2191 AGTACT AGT^ACT
SmaI 2314 CCCGGG CCC^GGG
SmlI 1183 CTTGAG C^T[CT][GA]AG
SpeI 3460 ACTAGT A^CTAGT
SphI 1578 GCATGC GCATG^C
Sse8387I 3063 CCTGCAGG CCTGCA^GG
SspBI 1722 TGTACA T^GTACA
StuI 1252 AGGCCT AGG^CCT
XbaI 3026 TCTAGA T^CTAGA
XhoII 2512 AGATCC [GA]^GATC[CT]
XmaCI 2314 CCCGGG C^CCGGG
XmaI 2314 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AlwI 2513 GATCC GGATC(4/5)
2530 GGATC
BclI 2670 TGATCA T^GATCA
2734 TGATCA
EaeI 1612 TGGCCA [CT]^GGCC[GA]
1740 CGGCCA
FbaI 2670 TGATCA T^GATCA
2734 TGATCA
HincII 3545 GTCAAC GT[CT]^[GA]AC
3668 GTTGAC
HindII 3545 GTCAAC GT[CT]^[GA]AC
3668 GTTGAC
HindIII 2825 AAGCTT A^AGCTT
3770 AAGCTT
NaeI 1738 GCCGGC GCC^GGC
3020 GCCGGC
NdeI 2413 CATATG CA^TATG
3134 CATATG
NgoMIV 1738 GCCGGC G^CCGGC
3020 GCCGGC
NspI 1578 GCATGC [GA]CATG^[CT]
1690 GCATGT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccI AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrFI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfiI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI