##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BanII BbeI
BbrPI BbsI BcgI BclI BcnI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CfoI
ClaI CpoI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 1412 AAATTT [GA]^AATT[CT]
AlwNI 1071 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1412 AAATTT [GA]^AATT[CT]
AspHI 1303 GTGCTC G[AT]GC[AT]^C
BamHI 421 GGATCC G^GATCC
BbvCI 1026 GCTGAGG CCTCAGC(-5/-2)
BciVI 1157 GGATAC GTATCC(6/5)
BglI 251 GCCATCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 883 GCTAAGC GC^T[ACGT]AGC
BpmI 448 CTCCAG CTGGAG(16/14)
Bpu1102I 883 GCTAAGC GC^T[ACGT]AGC
BsaI 203 GGTCTC GGTCTC(1/5)
BseRI 704 GAGGAG GAGGAG(10/8)
BsiEI 214 CGGTCG CG[GA][CT]^CG
BsiHKAI 1303 GTGCTC G[AT]GC[AT]^C
BsmI 951 GAATGC GAATGC(1/-1)
BspMI 1285 ACCTGC ACCTGC(4/8)
BsrDI 544 CATTGC GCAATG(2/0)
BsrFI 1325 GCCGGT [GA]^CCGG[CT]
BstYI 421 GGATCC [GA]^GATC[CT]
Cac8I 26 GCTGGC GC[ACGT]^[ACGT]GC
CelII 883 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1325 GCCGGT [GA]^CCGG[CT]
DraI 1275 TTTAAA TTT^AAA
DraII 1072 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 376 GAAGAG CTCTTC(1/4)
EcoO109I 1072 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 585 ATGCAT ATGCA^T
HapII 1326 CCGG C^CGG
HgaI 318 GCGTC GACGC(5/10)
HpaI 1098 GTTAAC GTT^AAC
HpaII 1326 CCGG C^CGG
Ksp632I 376 GAAGAG CTCTTC(1/4)
MflI 421 GGATCC [GA]^GATC[CT]
MspI 1326 CCGG C^CGG
NdeI 588 CATATG CA^TATG
NsiI 585 ATGCAT ATGCA^T
NspI 276 ACATGC [GA]CATG^[CT]
PflMI 1070 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 571 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 376 GAAGAGC GCTCTTC(1/4)
ScaI 912 AGTACT AGT^ACT
Tsp45I 1175 GTGAC ^GT[GC]AC
Van91I 1070 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 297 CCAAGCCAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 421 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 8 enzymes) #
###################################
BfaI 787 CTAG C^TAG
927 CTAG
BsrI 690 ACTGG ACTGG(1/-1)
302 CCAGT
BstXI 411 CCACAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCATTTTTGTGG
HindIII 715 AAGCTT A^AGCTT
721 AAGCTT
MaeI 787 CTAG C^TAG
927 CTAG
MaeIII 1175 GTGAC ^GT[ACGT]AC
1293 GTTAC
SfcI 819 CTGTAG C^T[GA][CT]AG
1062 CTGTAG
TfiI 346 GATTC G^A[AT]TC
1007 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AflII AluI AlwI
BbvI BfrI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MboI MboII MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI Tru9I
TseI Tsp509I TspRI