################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BciVI BclI BlnI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI FspI HpaI KasI KpnI MfeI MluI MroI MunI NarI NdeI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 1028 GTCTAC GT^[AC][GT]AC AocI 3721 CCTCAGG CC^T[ACGT]AGG AspI 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1309 TGGCCA TGG^CCA BanI 1221 GGCACC G^G[CT][GA]CC BbrPI 1326 CACGTG CAC^GTG BsaAI 1326 CACGTG [CT]AC^GT[GA] BsaBI 2135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 6 CGGCCG CG[GA][CT]^CG BspHI 4704 TCATGA T^CATGA BspMI 40 GCAGGT ACCTGC(4/8) BsrFI 4165 GCCGGC [GA]^CCGG[CT] BstAPI 4642 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 3721 CCTCAGG CC^T[ACGT]AGG Cfr10I 4165 GCCGGC [GA]^CCGG[CT] EagI 6 CGGCCG C^GGCCG EclXI 6 CGGCCG C^GGCCG Eco52I 6 CGGCCG C^GGCCG Eco81I 3721 CCTCAGG CC^T[ACGT]AGG EcoRI 3267 GAATTC G^AATTC HaeII 1241 GGCGCT [GA]GCGC^[CT] KspI 9 CCGCGG CCGC^GG MamI 2135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1309 TGGCCA TGG^CCA MscI 1309 TGGCCA TGG^CCA NaeI 4165 GCCGGC GCC^GGC NgoMIV 4165 GCCGGC G^CCGGC NheI 1349 GCTAGC G^CTAGC NotI 5 GCGGCCGC GC^GGCCGC PaeR7I 1774 CTCGAG C^TCGAG PflFI 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1326 CACGTG CAC^GTG PmlI 1326 CACGTG CAC^GTG PshAI 4228 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4704 TCATGA T^CATGA SacII 9 CCGCGG CCGC^GG SbfI 587 CCTGCAGG CCTGCA^GG ScaI 2394 AGTACT AGT^ACT SfiI 1301 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 3376 CCCGGG CCC^GGG Sse8387I 587 CCTGCAGG CCTGCA^GG StuI 5420 AGGCCT AGG^CCT SwaI 4601 ATTTAAAT ATTT^AAAT Tth111I 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1774 CTCGAG C^TCGAG XmaCI 3376 CCCGGG C^CCGGG XmaI 3376 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccII 10 CGCG CG^CG 3548 CGCG AflIII 365 ACGTGT A^C[GA][CT]GT 2476 ACGTGT ApaI 308 GGGCCC GGGCC^C 4963 GGGCCC BcgI 553 CGAGGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3999 GCACAGATGTCG BsmI 3596 GAATGC GAATGC(1/-1) 5497 GCATTC BstUI 10 CGCG CG^CG 3548 CGCG CfoI 1242 GCGC GCG^C 4576 GCGC DrdI 1374 GACCAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3632 GACACTGGAGTC HhaI 1242 GCGC GCG^C 4576 GCGC HinP1I 1242 GCGC G^CGC 4576 GCGC MvnI 10 CGCG CG^CG 3548 CGCG PflMI 3254 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3398 CCAGAAGCTGG Van91I 3254 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3398 CCAGAAGCTGG XcmI 1129 CCATGAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3082 CCATGCAGATCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AcsI AcyI AfaI AflII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BfrI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI