##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BciVI BclI
BlnI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI MfeI
MluI MroI MunI NarI NdeI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1028 GTCTAC GT^[AC][GT]AC
AocI 3721 CCTCAGG CC^T[ACGT]AGG
AspI 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1309 TGGCCA TGG^CCA
BanI 1221 GGCACC G^G[CT][GA]CC
BbrPI 1326 CACGTG CAC^GTG
BsaAI 1326 CACGTG [CT]AC^GT[GA]
BsaBI 2135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 6 CGGCCG CG[GA][CT]^CG
BspHI 4704 TCATGA T^CATGA
BspMI 40 GCAGGT ACCTGC(4/8)
BsrFI 4165 GCCGGC [GA]^CCGG[CT]
BstAPI 4642 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3721 CCTCAGG CC^T[ACGT]AGG
Cfr10I 4165 GCCGGC [GA]^CCGG[CT]
EagI 6 CGGCCG C^GGCCG
EclXI 6 CGGCCG C^GGCCG
Eco52I 6 CGGCCG C^GGCCG
Eco81I 3721 CCTCAGG CC^T[ACGT]AGG
EcoRI 3267 GAATTC G^AATTC
HaeII 1241 GGCGCT [GA]GCGC^[CT]
KspI 9 CCGCGG CCGC^GG
MamI 2135 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1309 TGGCCA TGG^CCA
MscI 1309 TGGCCA TGG^CCA
NaeI 4165 GCCGGC GCC^GGC
NgoMIV 4165 GCCGGC G^CCGGC
NheI 1349 GCTAGC G^CTAGC
NotI 5 GCGGCCGC GC^GGCCGC
PaeR7I 1774 CTCGAG C^TCGAG
PflFI 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1326 CACGTG CAC^GTG
PmlI 1326 CACGTG CAC^GTG
PshAI 4228 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4704 TCATGA T^CATGA
SacII 9 CCGCGG CCGC^GG
SbfI 587 CCTGCAGG CCTGCA^GG
ScaI 2394 AGTACT AGT^ACT
SfiI 1301 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3376 CCCGGG CCC^GGG
Sse8387I 587 CCTGCAGG CCTGCA^GG
StuI 5420 AGGCCT AGG^CCT
SwaI 4601 ATTTAAAT ATTT^AAAT
Tth111I 3381 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1774 CTCGAG C^TCGAG
XmaCI 3376 CCCGGG C^CCGGG
XmaI 3376 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 10 CGCG CG^CG
3548 CGCG
AflIII 365 ACGTGT A^C[GA][CT]GT
2476 ACGTGT
ApaI 308 GGGCCC GGGCC^C
4963 GGGCCC
BcgI 553 CGAGGAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3999 GCACAGATGTCG
BsmI 3596 GAATGC GAATGC(1/-1)
5497 GCATTC
BstUI 10 CGCG CG^CG
3548 CGCG
CfoI 1242 GCGC GCG^C
4576 GCGC
DrdI 1374 GACCAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3632 GACACTGGAGTC
HhaI 1242 GCGC GCG^C
4576 GCGC
HinP1I 1242 GCGC G^CGC
4576 GCGC
MvnI 10 CGCG CG^CG
3548 CGCG
PflMI 3254 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3398 CCAGAAGCTGG
Van91I 3254 CCAAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3398 CCAGAAGCTGG
XcmI 1129 CCATGAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3082 CCATGCAGATCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BfrI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI