##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BbeI BbrPI BcgI
BfrI BsaAI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI BssHII BstBI ClaI CpoI
DraIII EagI Eam1105I EclXI Eco52I
EcoT22I FseI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3328 GACGTC GACGT^C
Aor51HI 1153 AGCGCT AGC^GCT
Asp700I 1179 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3328 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 462 TGCGCA TGC^GCA
BamHI 3080 GGATCC G^GATCC
BanI 1428 GGTGCC G^G[CT][GA]CC
BciVI 754 GTATCC GTATCC(6/5)
BclI 85 TGATCA T^GATCA
BglI 432 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2537 AGATCT A^GATCT
BsaWI 757 TCCGGT [AT]^CCGG[AT]
BsmBI 3941 GAGACG CGTCTC(1/5)
Bsp1407I 4349 TGTACA T^GTACA
BsrFI 1904 GCCGGC [GA]^CCGG[CT]
BsrGI 4349 TGTACA T^GTACA
BssSI 49 CTCGTG CACGAG(-5/-1)
Bst1107I 146 GTATAC GTA^TAC
BstAPI 2472 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 760 GGTGACC G^GT[ACGT]ACC
BstPI 760 GGTGACC G^GT[ACGT]ACC
BstZ17I 146 GTATAC GTA^TAC
Cfr10I 1904 GCCGGC [GA]^CCGG[CT]
DrdI 2788 GACCTGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1153 AGCGCT AGC^GCT
EcoNI 4173 CCTACCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 760 GGTGACC G^GT[ACGT]ACC
EcoRI 293 GAATTC G^AATTC
EcoRV 2923 GATATC GAT^ATC
FbaI 85 TGATCA T^GATCA
FspI 462 TGCGCA TGC^GCA
HindIII 1267 AAGCTT A^AGCTT
NaeI 1904 GCCGGC GCC^GGC
NgoMIV 1904 GCCGGC G^CCGGC
NruI 1974 TCGCGA TCG^CGA
NspI 2765 GCATGC [GA]CATG^[CT]
PflFI 3328 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3621 GAGCTC GAGCT^C
SphI 2765 GCATGC GCATG^C
SspBI 4349 TGTACA T^GTACA
SspI 1993 AATATT AAT^ATT
Tth111I 3328 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 926 TCTAGA T^CTAGA
XcmI 2897 CCAAAGAACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1179 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 833 CCTCAGG CC^T[ACGT]AGG
2167 CCTGAGG
ApaI 220 GGGCCC GGGCC^C
439 GGGCCC
AvrII 812 CCTAGG C^CTAGG
1224 CCTAGG
BbvCI 1027 CCTCAGC CCTCAGC(-5/-2)
1551 CCTCAGC
BcnI 1190 CCCGG CC^[GC]GG
3084 CCCGG
BlnI 812 CCTAGG C^CTAGG
1224 CCTAGG
BlpI 3096 GCTCAGC GC^T[ACGT]AGC
4498 GCTGAGC
BpmI 352 CTCCAG CTGGAG(16/14)
1911 CTGGAG
Bpu1102I 3096 GCTCAGC GC^T[ACGT]AGC
4498 GCTGAGC
BsmI 3436 GCATTC GAATGC(1/-1)
4456 GCATTC
BsrBI 1408 GAGCGG CCGCTC(-3/-3)
2857 GAGCGG
Bsu36I 833 CCTCAGG CC^T[ACGT]AGG
2167 CCTGAGG
CelII 3096 GCTCAGC GC^T[ACGT]AGC
4498 GCTGAGC
Eco81I 833 CCTCAGG CC^T[ACGT]AGG
2167 CCTGAGG
NciI 1190 CCCGG CC^[GC]GG
3084 CCCGG
NcoI 2414 CCATGG C^CATGG
4014 CCATGG
PaeR7I 642 CTCGAG C^TCGAG
3322 CTCGAG
PpuMI 198 GGGTCCT [GA]G^G[AT]CC[CT]
2795 GGGTCCC
SapI 1854 GAAGAGC GCTCTTC(1/4)
3604 GAAGAGC
ScaI 1844 AGTACT AGT^ACT
3761 AGTACT
XhoI 642 CTCGAG C^TCGAG
3322 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvI BfaI
BmyI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII