##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AspI AvrII BbrPI BcgI
BciVI BfrI BlnI BlpI Bpu1102I
BsaWI BseAI BsgI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BssHII BssSI BstBI BstEII BstPI
BstXI CelII ClaI CpoI DrdI
Eam1105I Eco47III EcoNI EcoO65I HgaI
KpnI MluI MroI NheI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PvuI RcaI RsrII SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I StuI SwaI Tth111I Van91I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 312 GGCGCC G[GA]^CG[CT]C
AflIII 2923 ACGTGT A^C[GA][CT]GT
Alw44I 2243 GTGCAC G^TGCAC
AlwNI 2689 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2779 CCTGAGG CC^T[ACGT]AGG
ApaLI 2243 GTGCAC G^TGCAC
BalI 2472 TGGCCA TGG^CCA
BamHI 429 GGATCC G^GATCC
BanI 312 GGCGCC G^G[CT][GA]CC
BbeI 312 GGCGCC GGCGC^C
BbvCI 6 GCTGAGG CCTCAGC(-5/-2)
BglI 204 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2178 AGATCT A^GATCT
BsaAI 2922 TACGTG [CT]AC^GT[GA]
BsaBI 424 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 312 GGCGCC G[GA]^CG[CT]C
BsmI 2818 GCATTC GAATGC(1/-1)
BsrDI 1750 GCAATG GCAATG(2/0)
Bst1107I 2794 GTATAC GTA^TAC
BstZ17I 2794 GTATAC GTA^TAC
Bsu36I 2779 CCTGAGG CC^T[ACGT]AGG
DraIII 738 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2779 CCTGAGG CC^T[ACGT]AGG
EcoRV 2368 GATATC GAT^ATC
FseI 223 GGCCGGCC GGCCGG^CC
Hin1I 312 GGCGCC G[GA]^CG[CT]C
HindIII 827 AAGCTT A^AGCTT
HpaI 726 GTTAAC GTT^AAC
KasI 312 GGCGCC G^GCGCC
MamI 424 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3341 CAATTG C^AATTG
MluNI 2472 TGGCCA TGG^CCA
MscI 2472 TGGCCA TGG^CCA
MunI 3341 CAATTG C^AATTG
NaeI 224 GCCGGC GCC^GGC
NarI 312 GGCGCC GG^CGCC
NgoMIV 224 GCCGGC G^CCGGC
NotI 210 GCGGCCGC GC^GGCCGC
SacI 545 GAGCTC GAGCT^C
SalI 2141 GTCGAC G^TCGAC
SapI 1276 GAAGAGC GCTCTTC(1/4)
SfoI 312 GGCGCC GGC^GCC
SgrAI 308 CACCGGCG C[GA]^CCGG[CT]G
SmaI 246 CCCGGG CCC^GGG
SmlI 3659 CTCAAG C^T[CT][GA]AG
SphI 3349 GCATGC GCATG^C
XbaI 3508 TCTAGA T^CTAGA
XmaCI 246 CCCGGG C^CCGGG
XmaI 246 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AclI 1793 AACGTT AA^CGTT
3003 AACGTT
Asp700I 1150 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3871 GAAGGAATTC
AviII 47 TGCGCA TGC^GCA
3209 TGCGCA
BpmI 2155 CTCCAG CTGGAG(16/14)
2398 CTCCAG
BsaI 1655 GGTCTC GGTCTC(1/5)
1992 GGTCTC
BsiEI 164 CGGCCG CG[GA][CT]^CG
211 CGGCCG
BsrBI 1021 GAGCGG CCGCTC(-3/-3)
1243 GAGCGG
BstAPI 2274 GCACATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2809 GCATAAAATGC
EagI 164 CGGCCG C^GGCCG
211 CGGCCG
EclXI 164 CGGCCG C^GGCCG
211 CGGCCG
Eco52I 164 CGGCCG C^GGCCG
211 CGGCCG
EcoT22I 558 ATGCAT ATGCA^T
2816 ATGCAT
FspI 47 TGCGCA TGC^GCA
3209 TGCGCA
HaeII 312 GGCGCC [GA]GCGC^[CT]
350 AGCGCC
NcoI 450 CCATGG C^CATGG
851 CCATGG
NdeI 1363 CATATG CA^TATG
2801 CATATG
NsiI 558 ATGCAT ATGCA^T
2816 ATGCAT
Psp1406I 1793 AACGTT AA^CGTT
3003 AACGTT
PstI 1145 CTGCAG CTGCA^G
2174 CTGCAG
PvuII 3686 CAGCTG CAG^CTG
3892 CAGCTG
Tsp45I 304 GTGAC ^GT[GC]AC
3280 GTGAC
XmnI 1150 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3871 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BanII BbsI
BbvI BclI BcnI BfaI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PshBI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII