################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AflII AhdI AseI AsnI Asp700I AspEI AvrII BbrPI BciVI BclI BfrI BlnI BsaAI BsiWI Bsp1407I BspDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI DraI DraIII Eam1105I EcoNI EcoO65I EcoRI FbaI FseI HpaI MfeI MunI NdeI NotI NruI NspV PacI PmaCI PmeI PmlI PshAI PshBI SacI SalI SbfI SexAI SfiI SfuI SnaBI Sse8387I SspBI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AclI 4194 AACGTT AA^CGTT AgeI 819 ACCGGT A^CCGGT AlwNI 3314 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1430 AGCGCT AGC^GCT AscI 565 GGCGCGCC GG^CGCGCC AspI 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 4077 GGATCC G^GATCC BbvCI 1274 GCTGAGG CCTCAGC(-5/-2) BcgI 1294 GCACCAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 4168 AGATCT A^GATCT BlpI 915 GCTAAGC GC^T[ACGT]AGC Bpu1102I 915 GCTAAGC GC^T[ACGT]AGC BsaBI 2250 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 2297 TCATGA T^CATGA BsrBI 2005 CCGCTC CCGCTC(-3/-3) BstAPI 1935 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 915 GCTAAGC GC^T[ACGT]AGC DrdI 2442 GACGCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1008 CGGCCG C^GGCCG EclXI 1008 CGGCCG C^GGCCG Eco47III 1430 AGCGCT AGC^GCT Eco52I 1008 CGGCCG C^GGCCG EcoRV 2485 GATATC GAT^ATC HincII 3511 GTTGAC GT[CT]^[GA]AC HindII 3511 GTTGAC GT[CT]^[GA]AC HindIII 4130 AAGCTT A^AGCTT MamI 2250 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 500 ACGCGT A^CGCGT NcoI 4035 CCATGG C^CATGG NheI 180 GCTAGC G^CTAGC PaeR7I 3132 CTCGAG C^TCGAG PflFI 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 819 ACCGGT A^CCGGT Psp1406I 4194 AACGTT AA^CGTT PvuI 437 CGATCG CGAT^CG RcaI 2297 TCATGA T^CATGA SapI 595 GCTCTTC GCTCTTC(1/4) ScaI 2908 AGTACT AGT^ACT SgrAI 1163 CGCCGGCG C[GA]^CCGG[CT]G SpeI 4141 ACTAGT A^CTAGT SspI 3405 AATATT AAT^ATT Tth111I 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3132 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 1787 GGTACC G^GTACC 1806 GGTACC AccIII 1713 TCCGGA T^CCGGA 2841 TCCGGA AocI 1038 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG Asp718I 1787 GGTACC G^GTACC 1806 GGTACC AviII 1211 TGCGCA TGC^GCA 2922 TGCGCA BseAI 1713 TCCGGA T^CCGGA 2841 TCCGGA BsgI 1423 CTGCAC GTGCAG(16/14) 3954 CTGCAC BsmI 1085 GAATGC GAATGC(1/-1) 2981 GCATTC BspEI 1713 TCCGGA T^CCGGA 2841 TCCGGA BspMI 1157 ACCTGC ACCTGC(4/8) 133 GCAGGT BsrDI 957 CATTGC GCAATG(2/0) 1567 GCAATG BssHII 566 GCGCGC G^CGCGC 1257 GCGCGC Bsu36I 1038 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG CpoI 1373 CGGACCG CG^G[AT]CCG 1385 CGGACCG EarI 596 CTCTTC CTCTTC(1/4) 1187 GAAGAG Eco81I 1038 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG FspI 1211 TGCGCA TGC^GCA 2922 TGCGCA KpnI 1787 GGTACC GGTAC^C 1806 GGTACC Ksp632I 596 CTCTTC CTCTTC(1/4) 1187 GAAGAG MroI 1713 TCCGGA T^CCGGA 2841 TCCGGA MseI 2141 TTAA T^TAA 2914 TTAA NaeI 1164 GCCGGC GCC^GGC 4518 GCCGGC NgoMIV 1164 GCCGGC G^CCGGC 4518 GCCGGC PflMI 3719 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4030 CCAGCCCATGG RsrII 1373 CGGACCG CG^G[AT]CCG 1385 CGGACCG SphI 1940 GCATGC GCATG^C 1944 GCATGC StuI 863 AGGCCT AGG^CCT 2917 AGGCCT Tru9I 2141 TTAA T^TAA 2914 TTAA Van91I 3719 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4030 CCAGCCCATGG XcmI 2807 CCAAAGGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4035 CCATGGCGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI ApaI ApaLI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspLU11I BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI