##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AflII AhdI AseI AsnI
Asp700I AspEI AvrII BbrPI BciVI
BclI BfrI BlnI BsaAI BsiWI
Bsp1407I BspDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DraIII Eam1105I EcoNI EcoO65I
EcoRI FbaI FseI HpaI MfeI
MunI NdeI NotI NruI NspV
PacI PmaCI PmeI PmlI PshAI
PshBI SacI SalI SbfI SexAI
SfiI SfuI SnaBI Sse8387I SspBI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 4194 AACGTT AA^CGTT
AgeI 819 ACCGGT A^CCGGT
AlwNI 3314 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1430 AGCGCT AGC^GCT
AscI 565 GGCGCGCC GG^CGCGCC
AspI 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4077 GGATCC G^GATCC
BbvCI 1274 GCTGAGG CCTCAGC(-5/-2)
BcgI 1294 GCACCAGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4168 AGATCT A^GATCT
BlpI 915 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 915 GCTAAGC GC^T[ACGT]AGC
BsaBI 2250 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2297 TCATGA T^CATGA
BsrBI 2005 CCGCTC CCGCTC(-3/-3)
BstAPI 1935 GCACAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 915 GCTAAGC GC^T[ACGT]AGC
DrdI 2442 GACGCGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1008 CGGCCG C^GGCCG
EclXI 1008 CGGCCG C^GGCCG
Eco47III 1430 AGCGCT AGC^GCT
Eco52I 1008 CGGCCG C^GGCCG
EcoRV 2485 GATATC GAT^ATC
HincII 3511 GTTGAC GT[CT]^[GA]AC
HindII 3511 GTTGAC GT[CT]^[GA]AC
HindIII 4130 AAGCTT A^AGCTT
MamI 2250 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 500 ACGCGT A^CGCGT
NcoI 4035 CCATGG C^CATGG
NheI 180 GCTAGC G^CTAGC
PaeR7I 3132 CTCGAG C^TCGAG
PflFI 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 819 ACCGGT A^CCGGT
Psp1406I 4194 AACGTT AA^CGTT
PvuI 437 CGATCG CGAT^CG
RcaI 2297 TCATGA T^CATGA
SapI 595 GCTCTTC GCTCTTC(1/4)
ScaI 2908 AGTACT AGT^ACT
SgrAI 1163 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4141 ACTAGT A^CTAGT
SspI 3405 AATATT AAT^ATT
Tth111I 760 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3132 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 1787 GGTACC G^GTACC
1806 GGTACC
AccIII 1713 TCCGGA T^CCGGA
2841 TCCGGA
AocI 1038 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
Asp718I 1787 GGTACC G^GTACC
1806 GGTACC
AviII 1211 TGCGCA TGC^GCA
2922 TGCGCA
BseAI 1713 TCCGGA T^CCGGA
2841 TCCGGA
BsgI 1423 CTGCAC GTGCAG(16/14)
3954 CTGCAC
BsmI 1085 GAATGC GAATGC(1/-1)
2981 GCATTC
BspEI 1713 TCCGGA T^CCGGA
2841 TCCGGA
BspMI 1157 ACCTGC ACCTGC(4/8)
133 GCAGGT
BsrDI 957 CATTGC GCAATG(2/0)
1567 GCAATG
BssHII 566 GCGCGC G^CGCGC
1257 GCGCGC
Bsu36I 1038 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
CpoI 1373 CGGACCG CG^G[AT]CCG
1385 CGGACCG
EarI 596 CTCTTC CTCTTC(1/4)
1187 GAAGAG
Eco81I 1038 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
FspI 1211 TGCGCA TGC^GCA
2922 TGCGCA
KpnI 1787 GGTACC GGTAC^C
1806 GGTACC
Ksp632I 596 CTCTTC CTCTTC(1/4)
1187 GAAGAG
MroI 1713 TCCGGA T^CCGGA
2841 TCCGGA
MseI 2141 TTAA T^TAA
2914 TTAA
NaeI 1164 GCCGGC GCC^GGC
4518 GCCGGC
NgoMIV 1164 GCCGGC G^CCGGC
4518 GCCGGC
PflMI 3719 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4030 CCAGCCCATGG
RsrII 1373 CGGACCG CG^G[AT]CCG
1385 CGGACCG
SphI 1940 GCATGC GCATG^C
1944 GCATGC
StuI 863 AGGCCT AGG^CCT
2917 AGGCCT
Tru9I 2141 TTAA T^TAA
2914 TTAA
Van91I 3719 CCATGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4030 CCAGCCCATGG
XcmI 2807 CCAAAGGTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4035 CCATGGCGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
ApaI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI