##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I BamHI BsaBI
BsiEI BsiWI BsmBI BspHI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI HindIII HpaI
KpnI MamI MluI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 2630 TCCGGA T^CCGGA
AcsI 396 GAATTC [GA]^AATT[CT]
AflII 2221 CTTAAG C^TTAAG
AhdI 1227 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 396 GAATTC [GA]^AATT[CT]
AspEI 1227 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 826 TGCGCA TGC^GCA
AvrII 3032 CCTAGG C^CTAGG
BbeI 76 GGCGCC GGCGC^C
BbrPI 2801 CACGTG CAC^GTG
BcgI 170 GCAGAAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 938 GGATAC GTATCC(6/5)
BfrI 2221 CTTAAG C^TTAAG
BglII 4209 AGATCT A^GATCT
BlnI 3032 CCTAGG C^CTAGG
BsaAI 2801 CACGTG [CT]AC^GT[GA]
BseAI 2630 TCCGGA T^CCGGA
BsmI 2532 GCATTC GAATGC(1/-1)
Bsp1407I 4051 TGTACA T^GTACA
BspDI 1797 ATCGAT AT^CGAT
BspEI 2630 TCCGGA T^CCGGA
BsrGI 4051 TGTACA T^GTACA
BssSI 1642 CTCGTG CACGAG(-5/-1)
ClaI 1797 ATCGAT AT^CGAT
Eam1105I 1227 GACAGACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 396 GAATTC G^AATTC
FspI 826 TGCGCA TGC^GCA
HgaI 1604 GACGC GACGC(5/10)
HincII 3796 GTTGAC GT[CT]^[GA]AC
HindII 3796 GTTGAC GT[CT]^[GA]AC
KasI 76 GGCGCC G^GCGCC
KspI 3478 CCGCGG CCGC^GG
MfeI 3786 CAATTG C^AATTG
MroI 2630 TCCGGA T^CCGGA
MunI 3786 CAATTG C^AATTG
NarI 76 GGCGCC GG^CGCC
NheI 857 GCTAGC G^CTAGC
PaeR7I 3958 CTCGAG C^TCGAG
PmaCI 2801 CACGTG CAC^GTG
PmlI 2801 CACGTG CAC^GTG
PshAI 4186 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 3478 CCGCGG CCGC^GG
SexAI 1944 ACCTGGT A^CC[AT]GGT
SfoI 76 GGCGCC GGC^GCC
SmaI 1813 CCCGGG CCC^GGG
SpeI 138 ACTAGT A^CTAGT
SspBI 4051 TGTACA T^GTACA
SspI 2871 AATATT AAT^ATT
XbaI 4434 TCTAGA T^CTAGA
XcmI 3305 CCATTTCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3958 CTCGAG C^TCGAG
XmaCI 1813 CCCGGG C^CCGGG
XmaI 1813 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 76 GGCGCC G[GA]^CG[CT]C
1604 GACGCC
AflIII 2802 ACGTGT A^C[GA][CT]GT
4141 ACACGT
AspI 2557 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2846 GACTGGGTC
BalI 1974 TGGCCA TGG^CCA
3317 TGGCCA
BbvCI 1482 CCTCAGC CCTCAGC(-5/-2)
2518 CCTCAGC
BclI 1792 TGATCA T^GATCA
3472 TGATCA
BglI 1031 GCCTACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3014 GCCCACAAGGC
BlpI 111 GCTGAGC GC^T[ACGT]AGC
960 GCTCAGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
960 GCTCAGC
BsaHI 76 GGCGCC G[GA]^CG[CT]C
1604 GACGCC
BsaWI 2630 TCCGGA [AT]^CCGG[AT]
4090 ACCGGA
BspMI 322 ACCTGC ACCTGC(4/8)
248 GCAGGT
BsrDI 3235 CATTGC GCAATG(2/0)
1199 GCAATG
BstAPI 1199 GCAATGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4390 GCACACGATGC
CelII 111 GCTGAGC GC^T[ACGT]AGC
960 GCTCAGC
FbaI 1792 TGATCA T^GATCA
3472 TGATCA
HaeII 76 GGCGCC [GA]GCGC^[CT]
3350 GGCGCT
Hin1I 76 GGCGCC G[GA]^CG[CT]C
1604 GACGCC
MluNI 1974 TGGCCA TGG^CCA
3317 TGGCCA
MscI 1974 TGGCCA TGG^CCA
3317 TGGCCA
NaeI 2811 GCCGGC GCC^GGC
3055 GCCGGC
NcoI 271 CCATGG C^CATGG
1373 CCATGG
NgoMIV 2811 GCCGGC G^CCGGC
3055 GCCGGC
NspI 820 GCATGC [GA]CATG^[CT]
3419 GCATGC
PflFI 2557 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2846 GACTGGGTC
PflMI 182 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
458 CCACACCTTGG
SphI 820 GCATGC GCATG^C
3419 GCATGC
StuI 2192 AGGCCT AGG^CCT
2362 AGGCCT
Tth111I 2557 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2846 GACTGGGTC
Van91I 182 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
458 CCACACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII