##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BciVI
BcnI BglI BglII BlnI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstUI Bsu36I
Cfr10I ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI HaeII HapII
HgaI Hin1I HpaI HpaII KasI
KpnI KspI MluI MroI MspI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 3537 ACATGT A^C[GA][CT]GT
Alw44I 3770 GTGCAC G^TGCAC
AlwNI 1229 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3770 GTGCAC G^TGCAC
AspI 1431 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3001 CTCGAG C^[CT]CG[GA]G
BamHI 4223 GGATCC G^GATCC
BanI 1708 GGCACC G^G[CT][GA]CC
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
BlpI 2100 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2100 GCTCAGC GC^T[ACGT]AGC
BsmBI 2262 GAGACG CGTCTC(1/5)
BsoBI 3001 CTCGAG C^[CT]CG[GA]G
BspLU11I 3537 ACATGT A^CATGT
BssSI 3309 CTCGTG CACGAG(-5/-1)
Bst1107I 4778 GTATAC GTA^TAC
BstZ17I 4778 GTATAC GTA^TAC
CelII 2100 GCTCAGC GC^T[ACGT]AGC
DrdI 1256 GACGAGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2657 CCATGG C^C[GA][CT]GG
EcoNI 2573 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 94 GAATTC G^AATTC
EcoRV 2062 GATATC GAT^ATC
EcoT22I 4830 ATGCAT ATGCA^T
FauI 2432 CCCGC CCCGC(4/6)
HincII 2791 GTCAAC GT[CT]^[GA]AC
HindII 2791 GTCAAC GT[CT]^[GA]AC
NcoI 2657 CCATGG C^CATGG
NsiI 4830 ATGCAT ATGCA^T
PaeR7I 3001 CTCGAG C^TCGAG
PflFI 1431 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2705 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 2857 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2645 ACCTGGT A^CC[AT]GGT
StuI 2612 AGGCCT AGG^CCT
SwaI 5134 ATTTAAAT ATTT^AAAT
Tth111I 1431 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2636 CCATGAATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3001 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 3387 GTAGAC GT^[AC][GT]AC
4778 GTATAC
BalI 2520 TGGCCA TGG^CCA
3093 TGGCCA
BanII 233 GAGCTC G[GA]GC[CT]^C
1282 GAGCTC
BclI 1817 TGATCA T^GATCA
2148 TGATCA
BsaAI 729 TACGTG [CT]AC^GT[GA]
4360 TACGTG
BsaBI 2389 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3284 GATAGCAATC
BseRI 2324 CTCCTC GAGGAG(10/8)
4917 CTCCTC
Bsp1407I 3764 TGTACA T^GTACA
3937 TGTACA
BspMI 3489 GCAGGT ACCTGC(4/8)
4965 GCAGGT
BsrDI 1439 CATTGC GCAATG(2/0)
4686 CATTGC
BsrGI 3764 TGTACA T^GTACA
3937 TGTACA
BstXI 2816 CCAAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4085 CCAATTGTTTGG
CfoI 864 GCGC GCG^C
1143 GCGC
DraII 881 AGGCCCT [GA]G^G[ACGT]CC[CT]
2705 AGGTCCC
EaeI 2520 TGGCCA [CT]^GGCC[GA]
3093 TGGCCA
EcoO109I 881 AGGCCCT [GA]G^G[ACGT]CC[CT]
2705 AGGTCCC
EcoT14I 717 CCTTGG C^C[AT][AT]GG
2657 CCATGG
FbaI 1817 TGATCA T^GATCA
2148 TGATCA
HhaI 864 GCGC GCG^C
1143 GCGC
HinP1I 864 GCGC G^CGC
1143 GCGC
MamI 2389 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3284 GATAGCAATC
MluNI 2520 TGGCCA TGG^CCA
3093 TGGCCA
MscI 2520 TGGCCA TGG^CCA
3093 TGGCCA
MslI 160 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2631 CATGTCCATG
SacI 233 GAGCTC GAGCT^C
1282 GAGCTC
SapI 2541 GAAGAGC GCTCTTC(1/4)
2489 GCTCTTC
SpeI 3247 ACTAGT A^CTAGT
3917 ACTAGT
SspBI 3764 TGTACA T^GTACA
3937 TGTACA
SspI 4503 AATATT AAT^ATT
4544 AATATT
StyI 717 CCTTGG C^C[AT][AT]GG
2657 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BbsI BbvI BfaI
BfrI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrBI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MspA1I
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PshBI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII XmnI