##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflIII AgeI Aor51HI ApoI AscI
AviII BcgI BciVI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraI DraIII
Eco47III EcoRI FseI FspI HpaI
MamI MfeI MluI MroI MunI
NdeI NotI NruI PacI PaeR7I
PinAI PmeI Psp1406I PvuI RcaI
RsrII SalI ScaI SgrAI SnaBI
SpeI SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 5018 CTTAAG C^TTAAG
AhdI 209 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3337 ATTAAT AT^TAAT
AsnI 3337 ATTAAT AT^TAAT
AspEI 209 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1988 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 40 GGATCC G^GATCC
BbeI 2384 GGCGCC GGCGC^C
BclI 2988 TGATCA T^GATCA
BfrI 5018 CTTAAG C^TTAAG
BglII 831 AGATCT A^GATCT
BsmBI 1031 CGTCTC CGTCTC(1/5)
BstAPI 555 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3392 TTCGAA TT^CGAA
Eam1105I 209 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2591 GATATC GAT^ATC
EcoT22I 3696 ATGCAT ATGCA^T
FbaI 2988 TGATCA T^GATCA
HindIII 4813 AAGCTT A^AGCTT
KasI 2384 GGCGCC G^GCGCC
NarI 2384 GGCGCC GG^CGCC
NheI 1307 GCTAGC G^CTAGC
NsiI 3696 ATGCAT ATGCA^T
NspV 3392 TTCGAA TT^CGAA
PflFI 1988 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 764 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3337 ATTAAT AT^TAAT
SbfI 3292 CCTGCAGG CCTGCA^GG
SfiI 5095 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2384 GGCGCC GGC^GCC
SfuI 3392 TTCGAA TT^CGAA
SphI 3873 GCATGC GCATG^C
Sse8387I 3292 CCTGCAGG CCTGCA^GG
SspI 4896 AATATT AAT^ATT
Tth111I 1988 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 396 GGTACC G^GTACC
3158 GGTACC
Alw44I 239 GTGCAC G^TGCAC
1745 GTGCAC
ApaLI 239 GTGCAC G^TGCAC
1745 GTGCAC
Asp700I 1876 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2881 GAAGTTCTTC
Asp718I 396 GGTACC G^GTACC
3158 GGTACC
BlpI 1052 GCTCAGC GC^T[ACGT]AGC
4693 GCTCAGC
Bpu1102I 1052 GCTCAGC GC^T[ACGT]AGC
4693 GCTCAGC
BsrDI 1951 CATTGC GCAATG(2/0)
2641 CATTGC
BssSI 344 CACGAG CACGAG(-5/-1)
2258 CTCGTG
BstEII 1618 GGTCACC G^GT[ACGT]ACC
3770 GGTTACC
BstPI 1618 GGTCACC G^GT[ACGT]ACC
3770 GGTTACC
BstXI 1338 CCAGCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3094 CCAGTGCAGTGG
CelII 1052 GCTCAGC GC^T[ACGT]AGC
4693 GCTCAGC
EcoO65I 1618 GGTCACC G^GT[ACGT]ACC
3770 GGTTACC
HincII 524 GTCAAC GT[CT]^[GA]AC
578 GTCAAC
HindII 524 GTCAAC GT[CT]^[GA]AC
578 GTCAAC
KpnI 396 GGTACC GGTAC^C
3158 GGTACC
KspI 229 CCGCGG CCGC^GG
304 CCGCGG
NaeI 1105 GCCGGC GCC^GGC
4759 GCCGGC
NgoMIV 1105 GCCGGC G^CCGGC
4759 GCCGGC
SacI 1913 GAGCTC GAGCT^C
4770 GAGCTC
SacII 229 CCGCGG CCGC^GG
304 CCGCGG
SexAI 210 ACCTGGT A^CC[AT]GGT
4348 ACCAGGT
SmaI 465 CCCGGG CCC^GGG
5114 CCCGGG
StuI 2771 AGGCCT AGG^CCT
4432 AGGCCT
TfiI 1206 GAATC G^A[AT]TC
3403 GATTC
Tsp509I 3448 AATT ^AATT
4004 AATT
XmaCI 465 CCCGGG C^CCGGG
5114 CCCGGG
XmaI 465 CCCGGG C^CCGGG
5114 CCCGGG
XmnI 1876 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2881 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII