##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AviII BalI
BamHI BbeI BbrPI BclI BglII
BseAI BsiEI BsiWI BsmBI BspEI
BspLU11I BsrDI Bst1107I BstAPI BstBI
BstZ17I CpoI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FbaI FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NarI NotI NruI NsiI
NspV PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 2056 GGCGTC G[GA]^CG[CT]C
AflII 4381 CTTAAG C^TTAAG
AflIII 1308 ACGTGT A^C[GA][CT]GT
AhdI 2511 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2969 GTGCAC G^TGCAC
AocI 300 CCTGAGG CC^T[ACGT]AGG
ApaLI 2969 GTGCAC G^TGCAC
AspEI 2511 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1112 CTCGAG C^[CT]CG[GA]G
AvrII 2712 CCTAGG C^CTAGG
BcgI 2163 CGATTCTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2468 GGATAC GTATCC(6/5)
BfrI 4381 CTTAAG C^TTAAG
BglI 118 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2712 CCTAGG C^CTAGG
BsaBI 2557 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2056 GGCGTC G[GA]^CG[CT]C
BsaWI 3457 TCCGGT [AT]^CCGG[AT]
BsmI 2795 GAATGC GAATGC(1/-1)
BsoBI 1112 CTCGAG C^[CT]CG[GA]G
BspHI 4422 TCATGA T^CATGA
BsrBI 209 CCGCTC CCGCTC(-3/-3)
BsrFI 14 GCCGGC [GA]^CCGG[CT]
BstEII 4781 GGTGACC G^GT[ACGT]ACC
BstPI 4781 GGTGACC G^GT[ACGT]ACC
BstXI 705 CCACTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 300 CCTGAGG CC^T[ACGT]AGG
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
EaeI 1880 TGGCCG [CT]^GGCC[GA]
Eam1105I 2511 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 300 CCTGAGG CC^T[ACGT]AGG
EcoNI 2539 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4781 GGTGACC G^GT[ACGT]ACC
FauI 2346 GCGGG CCCGC(4/6)
Hin1I 2056 GGCGTC G[GA]^CG[CT]C
HindIII 1315 AAGCTT A^AGCTT
HpaI 177 GTTAAC GTT^AAC
MamI 2557 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 14 GCCGGC GCC^GGC
NdeI 616 CATATG CA^TATG
NgoMIV 14 GCCGGC G^CCGGC
NheI 42 GCTAGC G^CTAGC
PacI 3546 TTAATTAA TTAAT^TAA
PaeR7I 1112 CTCGAG C^TCGAG
PflMI 1872 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2850 CTGCAG CTGCA^G
RcaI 4422 TCATGA T^CATGA
ScaI 3638 AGTACT AGT^ACT
SfcI 2850 CTGCAG C^T[GA][CT]AG
SfiI 117 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 4261 AATATT AAT^ATT
Van91I 1872 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1868 CCAGCCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1112 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 37 CGCG CG^CG
39 CGCG
Asp700I 832 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2552 GAAAGGATTC
BanI 100 GGTGCC G^G[CT][GA]CC
364 GGTGCC
BbvCI 4372 CCTCAGC CCTCAGC(-5/-2)
1299 GCTGAGG
BlpI 31 GCTGAGC GC^T[ACGT]AGC
732 GCTAAGC
Bpu1102I 31 GCTGAGC GC^T[ACGT]AGC
732 GCTAAGC
BspDI 322 ATCGAT AT^CGAT
1264 ATCGAT
BssHII 36 GCGCGC G^CGCGC
38 GCGCGC
BstUI 37 CGCG CG^CG
39 CGCG
BstYI 701 AGATCC [GA]^GATC[CT]
1875 GGATCT
CelII 31 GCTGAGC GC^T[ACGT]AGC
732 GCTAAGC
ClaI 322 ATCGAT AT^CGAT
1264 ATCGAT
DraI 3496 TTTAAA TTT^AAA
4256 TTTAAA
DraIII 1716 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4459 CACAGGGTG
DrdI 1993 GACTCACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4685 GACCCCCAGGTC
EcoRI 3681 GAATTC G^AATTC
4342 GAATTC
HincII 177 GTTAAC GT[CT]^[GA]AC
3721 GTTGAC
HindII 177 GTTAAC GT[CT]^[GA]AC
3721 GTTGAC
MflI 701 AGATCC [GA]^GATC[CT]
1875 GGATCT
MvnI 37 CGCG CG^CG
39 CGCG
NcoI 1688 CCATGG C^CATGG
2174 CCATGG
PvuII 541 CAGCTG CAG^CTG
608 CAGCTG
SexAI 1976 ACCAGGT A^CC[AT]GGT
3413 ACCAGGT
StuI 3849 AGGCCT AGG^CCT
4109 AGGCCT
XhoII 701 AGATCC [GA]^GATC[CT]
1875 GGATCT
XmnI 832 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2552 GAAAGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BssSI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PshAI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XbaI