##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I ApaI ApaLI AscI
Asp718I AvrII BalI BbeI BbrPI
BciVI BclI BlnI BsaAI BsaBI
BsaHI BseAI BsiWI Bsp1407I BspDI
BspEI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I ClaI DrdI
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI NarI
NcoI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 3709 GTCTAC GT^[AC][GT]AC
AccII 34 CGCG CG^CG
AflII 4249 CTTAAG C^TTAAG
AhdI 1252 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1637 CCTCAGG CC^T[ACGT]AGG
AspEI 1252 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2926 TGCGCA TGC^GCA
BamHI 193 GGATCC G^GATCC
BcgI 547 GCAAGTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4249 CTTAAG C^TTAAG
BglII 4284 AGATCT A^GATCT
BsaWI 225 ACCGGA [AT]^CCGG[AT]
BsiEI 1125 CGGCCG CG[GA][CT]^CG
BsmI 177 GAATGC GAATGC(1/-1)
BsrDI 119 CATTGC GCAATG(2/0)
BsrFI 63 GCCGGC [GA]^CCGG[CT]
BstEII 1527 GGTAACC G^GT[ACGT]ACC
BstPI 1527 GGTAACC G^GT[ACGT]ACC
BstUI 34 CGCG CG^CG
Bsu36I 1637 CCTCAGG CC^T[ACGT]AGG
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
CpoI 222 CGGACCG CG^G[AT]CCG
EagI 1125 CGGCCG C^GGCCG
Eam1105I 1252 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1125 CGGCCG C^GGCCG
Eco52I 1125 CGGCCG C^GGCCG
Eco81I 1637 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1527 GGTAACC G^GT[ACGT]ACC
FspI 2926 TGCGCA TGC^GCA
MfeI 3164 CAATTG C^AATTG
MunI 3164 CAATTG C^AATTG
MvnI 34 CGCG CG^CG
NaeI 63 GCCGGC GCC^GGC
NdeI 4196 CATATG CA^TATG
NgoMIV 63 GCCGGC G^CCGGC
PpuMI 572 GGGACCT [GA]G^G[AT]CC[CT]
RsrII 222 CGGACCG CG^G[AT]CCG
SapI 3199 GCTCTTC GCTCTTC(1/4)
ScaI 2783 AGTACT AGT^ACT
SexAI 2970 ACCTGGT A^CC[AT]GGT
SphI 2929 GCATGC GCATG^C
StuI 1965 AGGCCT AGG^CCT
SwaI 3037 ATTTAAAT ATTT^AAAT
XbaI 2272 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 1905 GAATTT [GA]^AATT[CT]
2849 GAATTT
AflIII 3425 ACATGT A^C[GA][CT]GT
3972 ACATGT
Aor51HI 1170 AGCGCT AGC^GCT
2704 AGCGCT
ApoI 1905 GAATTT [GA]^AATT[CT]
2849 GAATTT
AseI 2473 ATTAAT AT^TAAT
3033 ATTAAT
AsnI 2473 ATTAAT AT^TAAT
3033 ATTAAT
Asp700I 94 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAGCCATTC
AspI 956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1055 GACACAGTC
BanI 2625 GGTGCC G^G[CT][GA]CC
4094 GGCACC
BbvCI 1887 CCTCAGC CCTCAGC(-5/-2)
2288 GCTGAGG
BglI 1929 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3618 GCCTTCATGGC
BlpI 407 GCTGAGC GC^T[ACGT]AGC
3287 GCTCAGC
Bpu1102I 407 GCTGAGC GC^T[ACGT]AGC
3287 GCTCAGC
BsmBI 1976 CGTCTC CGTCTC(1/5)
2278 GAGACG
BspHI 423 TCATGA T^CATGA
705 TCATGA
BspLU11I 3425 ACATGT A^CATGT
3972 ACATGT
BsrBI 364 CCGCTC CCGCTC(-3/-3)
47 GAGCGG
BstAPI 516 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
935 GCAGAAATTGC
CelII 407 GCTGAGC GC^T[ACGT]AGC
3287 GCTCAGC
DraI 3038 TTTAAA TTT^AAA
3338 TTTAAA
DraII 572 GGGACCT [GA]G^G[ACGT]CC[CT]
1684 AGGCCCC
DraIII 562 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
685 CACCATGTG
DsaI 639 CCGTGG C^C[GA][CT]GG
2685 CCACGG
Eco47III 1170 AGCGCT AGC^GCT
2704 AGCGCT
EcoO109I 572 GGGACCT [GA]G^G[ACGT]CC[CT]
1684 AGGCCCC
PflFI 956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1055 GACACAGTC
PshBI 2473 ATTAAT AT^TAAT
3033 ATTAAT
RcaI 423 TCATGA T^CATGA
705 TCATGA
SacI 410 GAGCTC GAGCT^C
1630 GAGCTC
SmaI 38 CCCGGG CCC^GGG
189 CCCGGG
TaqI 368 TCGA T^CGA
3745 TCGA
Tth111I 956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1055 GACACAGTC
TthHB8I 368 TCGA T^CGA
3745 TCGA
XcmI 1837 CCAGACCAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2685 CCACGGAGCATGTGG
XmaCI 38 CCCGGG C^CCGGG
189 CCCGGG
XmaI 38 CCCGGG C^CCGGG
189 CCCGGG
XmnI 94 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1014 GAAGCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII