##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII BanI
BbeI BglI BsaBI BsaWI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI Eam1105I
Eco47III Eco81I EcoO65I FseI FspI
HpaI KasI KpnI MamI MluI
MroI NarI NcoI NdeI NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 2755 CTTAAG C^TTAAG
ApaI 20 GGGCCC GGGCC^C
AvaI 4548 CTCGGG C^[CT]CG[GA]G
AvrII 1547 CCTAGG C^CTAGG
BalI 978 TGGCCA TGG^CCA
BamHI 1689 GGATCC G^GATCC
BbvCI 2015 CCTCAGC CCTCAGC(-5/-2)
BcgI 2135 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2755 CTTAAG C^TTAAG
BlnI 1547 CCTAGG C^CTAGG
BsiEI 66 CGGCCG CG[GA][CT]^CG
BsmBI 4296 CGTCTC CGTCTC(1/5)
BsmI 1669 GAATGC GAATGC(1/-1)
BsoBI 4548 CTCGGG C^[CT]CG[GA]G
BsrBI 189 CCGCTC CCGCTC(-3/-3)
BsrDI 1144 CATTGC GCAATG(2/0)
BstBI 2475 TTCGAA TT^CGAA
DraI 3738 TTTAAA TTT^AAA
DraIII 4382 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4795 GACGTAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 66 CGGCCG C^GGCCG
EclXI 66 CGGCCG C^GGCCG
Eco52I 66 CGGCCG C^GGCCG
EcoNI 2039 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1547 CCTAGG C^C[AT][AT]GG
MfeI 852 CAATTG C^AATTG
MluNI 978 TGGCCA TGG^CCA
MscI 978 TGGCCA TGG^CCA
MunI 852 CAATTG C^AATTG
NspV 2475 TTCGAA TT^CGAA
SacI 4129 GAGCTC GAGCT^C
ScaI 2123 AGTACT AGT^ACT
SfuI 2475 TTCGAA TT^CGAA
SpeI 539 ACTAGT A^CTAGT
SspI 4816 AATATT AAT^ATT
StyI 1547 CCTAGG C^C[AT][AT]GG
XbaI 4597 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 401 GACGTC GACGT^C
4294 GACGTC
BbrPI 2393 CACGTG CAC^GTG
4394 CACGTG
BciVI 2368 GGATAC GTATCC(6/5)
3521 GGATAC
BglII 460 AGATCT A^GATCT
3069 AGATCT
BsaAI 2393 CACGTG [CT]AC^GT[GA]
4394 CACGTG
BsrFI 127 GCCGGC [GA]^CCGG[CT]
288 GCCGGC
BstXI 406 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4466 CCACTTGAATGG
Cfr10I 127 GCCGGC [GA]^CCGG[CT]
288 GCCGGC
EcoRI 2825 GAATTC G^AATTC
4683 GAATTC
EcoT22I 607 ATGCAT ATGCA^T
3672 ATGCAT
HaeII 360 AGCGCC [GA]GCGC^[CT]
699 AGCGCC
HincII 783 GTCAAC GT[CT]^[GA]AC
3810 GTCAAC
HindII 783 GTCAAC GT[CT]^[GA]AC
3810 GTCAAC
HindIII 872 AAGCTT A^AGCTT
2909 AAGCTT
NaeI 127 GCCGGC GCC^GGC
288 GCCGGC
NgoMIV 127 GCCGGC G^CCGGC
288 GCCGGC
NsiI 607 ATGCAT ATGCA^T
3672 ATGCAT
PmaCI 2393 CACGTG CAC^GTG
4394 CACGTG
PmlI 2393 CACGTG CAC^GTG
4394 CACGTG
PstI 1493 CTGCAG CTGCA^G
4526 CTGCAG
PvuII 135 CAGCTG CAG^CTG
2140 CAGCTG
StuI 1096 AGGCCT AGG^CCT
2380 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AseI AsnI Asp700I AspHI
AvaII BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoRV FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI