################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## AatII AccIII AclI AflII AscI AseI AsnI Asp700I BclI BfrI BglII BseAI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstZ17I ClaI DraIII FbaI HindIII HpaI KspI MfeI MroI MunI NdeI NheI NruI PacI PmeI PshBI Psp1406I PvuI SacII SalI SbfI ScaI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 1843 GTCTAC GT^[AC][GT]AC AgeI 942 ACCGGT A^CCGGT Alw44I 3463 GTGCAC G^TGCAC ApaLI 3463 GTGCAC G^TGCAC AspI 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 698 TGCGCA TGC^GCA AvrII 2382 CCTAGG C^CTAGG BamHI 4084 GGATCC G^GATCC BbrPI 3886 CACGTG CAC^GTG BciVI 3402 GTATCC GTATCC(6/5) BlnI 2382 CCTAGG C^CTAGG BsaBI 3397 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 2012 TCATGA T^CATGA BstBI 3123 TTCGAA TT^CGAA CpoI 867 CGGTCCG CG^G[AT]CCG DraI 4946 TTTAAA TTT^AAA EagI 797 CGGCCG C^GGCCG EclXI 797 CGGCCG C^GGCCG Eco52I 797 CGGCCG C^GGCCG EcoRI 1525 GAATTC G^AATTC EcoRV 919 GATATC GAT^ATC EcoT22I 3963 ATGCAT ATGCA^T FseI 1882 GGCCGGCC GGCCGG^CC FspI 698 TGCGCA TGC^GCA MamI 3397 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 2172 ACGCGT A^CGCGT NotI 796 GCGGCCGC GC^GGCCGC NsiI 3963 ATGCAT ATGCA^T NspV 3123 TTCGAA TT^CGAA PaeR7I 2418 CTCGAG C^TCGAG PflFI 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2288 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 942 ACCGGT A^CCGGT PmaCI 3886 CACGTG CAC^GTG PmlI 3886 CACGTG CAC^GTG PshAI 3160 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2012 TCATGA T^CATGA RsrII 867 CGGTCCG CG^G[AT]CCG SapI 2253 GCTCTTC GCTCTTC(1/4) SexAI 2310 ACCTGGT A^CC[AT]GGT SfuI 3123 TTCGAA TT^CGAA SphI 1821 GCATGC GCATG^C SspI 4971 AATATT AAT^ATT StuI 498 AGGCCT AGG^CCT Tth111I 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2288 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2418 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 1667 GGTACC G^GTACC 3504 GGTACC AhdI 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1828 GACTACCAGTC Aor51HI 527 AGCGCT AGC^GCT 3686 AGCGCT Asp718I 1667 GGTACC G^GTACC 3504 GGTACC AspEI 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1828 GACTACCAGTC BbvCI 2686 GCTGAGG CCTCAGC(-5/-2) 4337 GCTGAGG BcgI 676 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1025 GCAATGTCATCG BglI 748 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2764 GCCCCCGGGGC BpmI 2215 CTGGAG CTGGAG(16/14) 3441 CTGGAG BsiEI 605 CGGTCG CG[GA][CT]^CG 797 CGGCCG BsmBI 1638 CGTCTC CGTCTC(1/5) 3911 GAGACG BssSI 684 CTCGTG CACGAG(-5/-1) 2221 CTCGTG Eam1105I 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1828 GACTACCAGTC Eco47III 527 AGCGCT AGC^GCT 3686 AGCGCT EcoNI 516 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3651 CCTGCTAGAGG KpnI 1667 GGTACC GGTAC^C 3504 GGTACC NaeI 753 GCCGGC GCC^GGC 1883 GCCGGC NgoMIV 753 GCCGGC G^CCGGC 1883 GCCGGC PstI 1592 CTGCAG CTGCA^G 2647 CTGCAG SfcI 1592 CTGCAG C^T[GA][CT]AG 2647 CTGCAG SfiI 747 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 2763 GGCCCCCGGGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 143 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmaCI XmaI