##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII AccIII AclI AflII AscI
AseI AsnI Asp700I BclI BfrI
BglII BseAI BsiWI BsmI BspDI
BspEI BssHII Bst1107I BstZ17I ClaI
DraIII FbaI HindIII HpaI KspI
MfeI MroI MunI NdeI NheI
NruI PacI PmeI PshBI Psp1406I
PvuI SacII SalI SbfI ScaI
SgrAI SnaBI SpeI Sse8387I SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1843 GTCTAC GT^[AC][GT]AC
AgeI 942 ACCGGT A^CCGGT
Alw44I 3463 GTGCAC G^TGCAC
ApaLI 3463 GTGCAC G^TGCAC
AspI 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 698 TGCGCA TGC^GCA
AvrII 2382 CCTAGG C^CTAGG
BamHI 4084 GGATCC G^GATCC
BbrPI 3886 CACGTG CAC^GTG
BciVI 3402 GTATCC GTATCC(6/5)
BlnI 2382 CCTAGG C^CTAGG
BsaBI 3397 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 2012 TCATGA T^CATGA
BstBI 3123 TTCGAA TT^CGAA
CpoI 867 CGGTCCG CG^G[AT]CCG
DraI 4946 TTTAAA TTT^AAA
EagI 797 CGGCCG C^GGCCG
EclXI 797 CGGCCG C^GGCCG
Eco52I 797 CGGCCG C^GGCCG
EcoRI 1525 GAATTC G^AATTC
EcoRV 919 GATATC GAT^ATC
EcoT22I 3963 ATGCAT ATGCA^T
FseI 1882 GGCCGGCC GGCCGG^CC
FspI 698 TGCGCA TGC^GCA
MamI 3397 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2172 ACGCGT A^CGCGT
NotI 796 GCGGCCGC GC^GGCCGC
NsiI 3963 ATGCAT ATGCA^T
NspV 3123 TTCGAA TT^CGAA
PaeR7I 2418 CTCGAG C^TCGAG
PflFI 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2288 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 942 ACCGGT A^CCGGT
PmaCI 3886 CACGTG CAC^GTG
PmlI 3886 CACGTG CAC^GTG
PshAI 3160 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2012 TCATGA T^CATGA
RsrII 867 CGGTCCG CG^G[AT]CCG
SapI 2253 GCTCTTC GCTCTTC(1/4)
SexAI 2310 ACCTGGT A^CC[AT]GGT
SfuI 3123 TTCGAA TT^CGAA
SphI 1821 GCATGC GCATG^C
SspI 4971 AATATT AAT^ATT
StuI 498 AGGCCT AGG^CCT
Tth111I 1483 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2288 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2418 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1667 GGTACC G^GTACC
3504 GGTACC
AhdI 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1828 GACTACCAGTC
Aor51HI 527 AGCGCT AGC^GCT
3686 AGCGCT
Asp718I 1667 GGTACC G^GTACC
3504 GGTACC
AspEI 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1828 GACTACCAGTC
BbvCI 2686 GCTGAGG CCTCAGC(-5/-2)
4337 GCTGAGG
BcgI 676 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1025 GCAATGTCATCG
BglI 748 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2764 GCCCCCGGGGC
BpmI 2215 CTGGAG CTGGAG(16/14)
3441 CTGGAG
BsiEI 605 CGGTCG CG[GA][CT]^CG
797 CGGCCG
BsmBI 1638 CGTCTC CGTCTC(1/5)
3911 GAGACG
BssSI 684 CTCGTG CACGAG(-5/-1)
2221 CTCGTG
Eam1105I 1606 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1828 GACTACCAGTC
Eco47III 527 AGCGCT AGC^GCT
3686 AGCGCT
EcoNI 516 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3651 CCTGCTAGAGG
KpnI 1667 GGTACC GGTAC^C
3504 GGTACC
NaeI 753 GCCGGC GCC^GGC
1883 GCCGGC
NgoMIV 753 GCCGGC G^CCGGC
1883 GCCGGC
PstI 1592 CTGCAG CTGCA^G
2647 CTGCAG
SfcI 1592 CTGCAG C^T[GA][CT]AG
2647 CTGCAG
SfiI 747 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2763 GGCCCCCGGGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfoI SmaI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI