##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AscI AseI AsnI Asp700I AspEI
AvrII BciVI BfrI BglII BlnI
BsaBI BseAI BsiWI BspDI BspEI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eam1105I EcoRV EcoT22I FseI
HincII HindII HindIII HpaI MamI
MluI MroI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI ScaI SfiI SfuI
SgrAI SnaBI SpeI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 489 GACGTC GACGT^C
Acc65I 3091 GGTACC G^GTACC
AocI 1125 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4310 AGCGCT AGC^GCT
Asp718I 3091 GGTACC G^GTACC
AviII 1290 TGCGCA TGC^GCA
BcgI 1326 CGATGGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 1764 GAGACG CGTCTC(1/5)
BsmI 1525 GAATGC GAATGC(1/-1)
Bsp1407I 5078 TGTACA T^GTACA
BspHI 2180 TCATGA T^CATGA
BsrGI 5078 TGTACA T^GTACA
BstEII 2722 GGTCACC G^GT[ACGT]ACC
BstPI 2722 GGTCACC G^GT[ACGT]ACC
Bsu36I 1125 CCTGAGG CC^T[ACGT]AGG
Eco47III 4310 AGCGCT AGC^GCT
Eco81I 1125 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2722 GGTCACC G^GT[ACGT]ACC
EcoRI 4770 GAATTC G^AATTC
FspI 1290 TGCGCA TGC^GCA
HgaI 1766 GACGC GACGC(5/10)
KpnI 3091 GGTACC GGTAC^C
KspI 158 CCGCGG CCGC^GG
MfeI 2454 CAATTG C^AATTG
MunI 2454 CAATTG C^AATTG
NaeI 214 GCCGGC GCC^GGC
NgoMIV 214 GCCGGC G^CCGGC
PaeR7I 3989 CTCGAG C^TCGAG
RcaI 2180 TCATGA T^CATGA
SacII 158 CCGCGG CCGC^GG
SbfI 1605 CCTGCAGG CCTGCA^GG
SexAI 3800 ACCTGGT A^CC[AT]GGT
SmaI 92 CCCGGG CCC^GGG
SphI 1191 GCATGC GCATG^C
Sse8387I 1605 CCTGCAGG CCTGCA^GG
SspBI 5078 TGTACA T^GTACA
XhoI 3989 CTCGAG C^TCGAG
XmaCI 92 CCCGGG C^CCGGG
XmaI 92 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 2929 AAATTT [GA]^AATT[CT]
4770 GAATTC
Alw44I 2791 GTGCAC G^TGCAC
4098 GTGCAC
ApaLI 2791 GTGCAC G^TGCAC
4098 GTGCAC
ApoI 2929 AAATTT [GA]^AATT[CT]
4770 GAATTC
BbeI 84 GGCGCC GGCGC^C
3059 GGCGCC
BbrPI 1931 CACGTG CAC^GTG
2203 CACGTG
BsaWI 4094 TCCGGT [AT]^CCGG[AT]
4493 ACCGGA
BspLU11I 563 ACATGT A^CATGT
3311 ACATGT
BsrFI 214 GCCGGC [GA]^CCGG[CT]
3224 ACCGGC
Cfr10I 214 GCCGGC [GA]^CCGG[CT]
3224 ACCGGC
EagI 61 CGGCCG C^GGCCG
4924 CGGCCG
EclXI 61 CGGCCG C^GGCCG
4924 CGGCCG
Eco52I 61 CGGCCG C^GGCCG
4924 CGGCCG
KasI 84 GGCGCC G^GCGCC
3059 GGCGCC
NarI 84 GGCGCC GG^CGCC
3059 GGCGCC
PmaCI 1931 CACGTG CAC^GTG
2203 CACGTG
PmlI 1931 CACGTG CAC^GTG
2203 CACGTG
SapI 4743 GAAGAGC GCTCTTC(1/4)
3447 GCTCTTC
SfoI 84 GGCGCC GGC^GCC
3059 GGCGCC
SspI 4957 AATATT AAT^ATT
5092 AATATT
TfiI 3476 GAATC G^A[AT]TC
4497 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssHII BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII