##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
AscI Asp700I Asp718I AspEI AviII
BbeI BbvCI BfrI BglII BsaI
BseAI BsiEI BspEI BspLU11I BssHII
Bst1107I BstZ17I EagI Eam1105I EclXI
Eco52I EcoNI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmeI
PvuI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SwaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 3377 GACGTC GACGT^C
AccII 1771 CGCG CG^CG
AclI 3250 AACGTT AA^CGTT
AcyI 3377 GACGTC G[GA]^CG[CT]C
AflIII 3831 ACGTGT A^C[GA][CT]GT
Aor51HI 3129 AGCGCT AGC^GCT
ApaI 1081 GGGCCC GGGCC^C
AvaI 1824 CTCGAG C^[CT]CG[GA]G
AvrII 4232 CCTAGG C^CTAGG
BamHI 2700 GGATCC G^GATCC
BanI 3136 GGTGCC G^G[CT][GA]CC
BbrPI 3830 CACGTG CAC^GTG
BclI 941 TGATCA T^GATCA
BglI 2504 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4232 CCTAGG C^CTAGG
BlpI 3125 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3125 GCTGAGC GC^T[ACGT]AGC
BsaHI 3377 GACGTC G[GA]^CG[CT]C
BsgI 2586 CTGCAC GTGCAG(16/14)
BsiWI 4296 CGTACG C^GTACG
BsmI 1749 GAATGC GAATGC(1/-1)
BsoBI 1824 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1175 TGTACA T^GTACA
BspDI 2966 ATCGAT AT^CGAT
BsrGI 1175 TGTACA T^GTACA
BstAPI 639 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1936 TTCGAA TT^CGAA
BstUI 1771 CGCG CG^CG
CelII 3125 GCTGAGC GC^T[ACGT]AGC
ClaI 2966 ATCGAT AT^CGAT
CpoI 1739 CGGTCCG CG^G[AT]CCG
DraIII 3096 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1236 CCACGG C^C[GA][CT]GG
Eco47III 3129 AGCGCT AGC^GCT
FbaI 941 TGATCA T^GATCA
Hin1I 3377 GACGTC G[GA]^CG[CT]C
MfeI 3503 CAATTG C^AATTG
MunI 3503 CAATTG C^AATTG
MvnI 1771 CGCG CG^CG
NspV 1936 TTCGAA TT^CGAA
PaeR7I 1824 CTCGAG C^TCGAG
PmaCI 3830 CACGTG CAC^GTG
PmlI 3830 CACGTG CAC^GTG
PpuMI 1719 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 3173 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3250 AACGTT AA^CGTT
RsrII 1739 CGGTCCG CG^G[AT]CCG
SacI 2776 GAGCTC GAGCT^C
SapI 2430 GCTCTTC GCTCTTC(1/4)
ScaI 88 AGTACT AGT^ACT
SfuI 1936 TTCGAA TT^CGAA
SmlI 1824 CTCGAG C^T[CT][GA]AG
SpeI 3737 ACTAGT A^CTAGT
SspBI 1175 TGTACA T^GTACA
XhoI 1824 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 601 GTCTAC GT^[AC][GT]AC
3029 GTCTAC
AseI 771 ATTAAT AT^TAAT
886 ATTAAT
AsnI 771 ATTAAT AT^TAAT
886 ATTAAT
BalI 2040 TGGCCA TGG^CCA
3606 TGGCCA
BcgI 3652 CGAAGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
648 GCACGAGCCTCG
BsaAI 583 TACGTG [CT]AC^GT[GA]
3830 CACGTG
BsaBI 936 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2701 GATCCTTATC
BsaWI 2951 TCCGGT [AT]^CCGG[AT]
3535 TCCGGT
BsmBI 626 CGTCTC CGTCTC(1/5)
2635 GAGACG
BspHI 473 TCATGA T^CATGA
3751 TCATGA
BspMI 1730 ACCTGC ACCTGC(4/8)
2518 ACCTGC
BsrBI 3666 CCGCTC CCGCTC(-3/-3)
1194 GAGCGG
BsrFI 685 ACCGGC [GA]^CCGG[CT]
4431 ACCGGC
BssSI 649 CACGAG CACGAG(-5/-1)
633 CTCGTG
BstYI 2572 GGATCT [GA]^GATC[CT]
2700 GGATCC
Cfr10I 685 ACCGGC [GA]^CCGG[CT]
4431 ACCGGC
DraI 4022 TTTAAA TTT^AAA
4199 TTTAAA
DraII 1080 AGGGCCC [GA]G^G[ACGT]CC[CT]
1719 GGGACCC
EaeI 2040 TGGCCA [CT]^GGCC[GA]
3606 TGGCCA
EarI 2431 CTCTTC CTCTTC(1/4)
3576 CTCTTC
EcoO109I 1080 AGGGCCC [GA]G^G[ACGT]CC[CT]
1719 GGGACCC
HaeII 2009 AGCGCC [GA]GCGC^[CT]
3129 AGCGCT
HgaI 2637 GACGC GACGC(5/10)
3508 GCGTC
Ksp632I 2431 CTCTTC CTCTTC(1/4)
3576 CTCTTC
MamI 936 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2701 GATCCTTATC
MflI 2572 GGATCT [GA]^GATC[CT]
2700 GGATCC
MluNI 2040 TGGCCA TGG^CCA
3606 TGGCCA
MscI 2040 TGGCCA TGG^CCA
3606 TGGCCA
NspI 1181 ACATGC [GA]CATG^[CT]
4320 GCATGT
PshBI 771 ATTAAT AT^TAAT
886 ATTAAT
RcaI 473 TCATGA T^CATGA
3751 TCATGA
StuI 2213 AGGCCT AGG^CCT
2935 AGGCCT
XbaI 191 TCTAGA T^CTAGA
1452 TCTAGA
XhoII 2572 GGATCT [GA]^GATC[CT]
2700 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AspI AvaII BanII BbsI
BbvI BciVI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DrdI
Eco81I EcoO65I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflFI
PflMI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI