##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AgeI Alw44I ApaLI
AscI Asp718I AviII AvrII BbrPI
BciVI BglI BlnI BsaBI BsiEI
BsiWI BsmBI BsrFI BssHII BstAPI
Cfr10I CpoI DraIII DrdI EagI
EclXI Eco52I EcoT22I FseI FspI
KpnI MamI MfeI MluI MunI
NaeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmaCI PmlI
Psp1406I PvuI RsrII SbfI SexAI
SfiI SgrAI SpeI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 606 GACGTC GACGT^C
AflII 563 CTTAAG C^TTAAG
AflIII 4697 ACATGT A^C[GA][CT]GT
AhdI 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3895 AGCGCT AGC^GCT
Asp700I 2414 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 111 TGGCCA TGG^CCA
BbeI 3177 GGCGCC GGCGC^C
BfrI 563 CTTAAG C^TTAAG
BglII 559 AGATCT A^GATCT
BspLU11I 4697 ACATGT A^CATGT
BsrBI 2745 GAGCGG CCGCTC(-3/-3)
BssSI 466 CACGAG CACGAG(-5/-1)
Bst1107I 1262 GTATAC GTA^TAC
BstBI 578 TTCGAA TT^CGAA
BstEII 1280 GGTCACC G^GT[ACGT]ACC
BstPI 1280 GGTCACC G^GT[ACGT]ACC
BstZ17I 1262 GTATAC GTA^TAC
Eam1105I 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3895 AGCGCT AGC^GCT
EcoNI 3181 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1280 GGTCACC G^GT[ACGT]ACC
EcoRI 3903 GAATTC G^AATTC
HpaI 2382 GTTAAC GTT^AAC
KasI 3177 GGCGCC G^GCGCC
KspI 2575 CCGCGG CCGC^GG
MluNI 111 TGGCCA TGG^CCA
MscI 111 TGGCCA TGG^CCA
NarI 3177 GGCGCC GG^CGCC
NcoI 4210 CCATGG C^CATGG
NspV 578 TTCGAA TT^CGAA
PflFI 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 103 CCATTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 1406 GTTTAAAC GTTT^AAAC
PshAI 1024 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 2575 CCGCGG CCGC^GG
SalI 3405 GTCGAC G^TCGAC
ScaI 4057 AGTACT AGT^ACT
SfoI 3177 GGCGCC GGC^GCC
SfuI 578 TTCGAA TT^CGAA
SmaI 1017 CCCGGG CCC^GGG
Tth111I 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 103 CCATTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1017 CCCGGG C^CCGGG
XmaI 1017 CCCGGG C^CCGGG
XmnI 2414 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1262 GTATAC GT^[AC][GT]AC
3405 GTCGAC
AccII 1421 CGCG CG^CG
2576 CGCG
AccIII 1910 TCCGGA T^CCGGA
4564 TCCGGA
AocI 1191 CCTGAGG CC^T[ACGT]AGG
4539 CCTCAGG
ApaI 3085 GGGCCC GGGCC^C
4510 GGGCCC
AseI 105 ATTAAT AT^TAAT
2331 ATTAAT
AsnI 105 ATTAAT AT^TAAT
2331 ATTAAT
BamHI 1216 GGATCC G^GATCC
3332 GGATCC
BanI 3177 GGCGCC G^G[CT][GA]CC
4239 GGTGCC
BbsI 528 GAAGAC GAAGAC(2/6)
3954 GAAGAC
BbvCI 597 GCTGAGG CCTCAGC(-5/-2)
653 GCTGAGG
BcgI 1041 CGACGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3521 CGAGAGGGCTGC
BclI 1600 TGATCA T^GATCA
1625 TGATCA
BlpI 2292 GCTAAGC GC^T[ACGT]AGC
3531 GCTCAGC
Bpu1102I 2292 GCTAAGC GC^T[ACGT]AGC
3531 GCTCAGC
BpuAI 528 GAAGAC GAAGAC(2/6)
3954 GAAGAC
BsaWI 1910 TCCGGA [AT]^CCGG[AT]
4564 TCCGGA
BseAI 1910 TCCGGA T^CCGGA
4564 TCCGGA
BsgI 912 CTGCAC GTGCAG(16/14)
2206 CTGCAC
Bsp1407I 2438 TGTACA T^GTACA
2473 TGTACA
BspDI 436 ATCGAT AT^CGAT
1636 ATCGAT
BspEI 1910 TCCGGA T^CCGGA
4564 TCCGGA
BspHI 338 TCATGA T^CATGA
2822 TCATGA
BsrGI 2438 TGTACA T^GTACA
2473 TGTACA
BstUI 1421 CGCG CG^CG
2576 CGCG
Bsu36I 1191 CCTGAGG CC^T[ACGT]AGG
4539 CCTCAGG
CelII 2292 GCTAAGC GC^T[ACGT]AGC
3531 GCTCAGC
ClaI 436 ATCGAT AT^CGAT
1636 ATCGAT
EaeI 111 TGGCCA [CT]^GGCC[GA]
4469 TGGCCG
Eco81I 1191 CCTGAGG CC^T[ACGT]AGG
4539 CCTCAGG
EcoRV 3776 GATATC GAT^ATC
4191 GATATC
FbaI 1600 TGATCA T^GATCA
1625 TGATCA
HgaI 3750 GACGC GACGC(5/10)
3403 GCGTC
HindIII 168 AAGCTT A^AGCTT
4559 AAGCTT
MroI 1910 TCCGGA T^CCGGA
4564 TCCGGA
MvnI 1421 CGCG CG^CG
2576 CGCG
NdeI 442 CATATG CA^TATG
646 CATATG
PpuMI 671 GGGTCCT [GA]G^G[AT]CC[CT]
3070 AGGACCT
PshBI 105 ATTAAT AT^TAAT
2331 ATTAAT
RcaI 338 TCATGA T^CATGA
2822 TCATGA
SapI 978 GAAGAGC GCTCTTC(1/4)
3536 GCTCTTC
SnaBI 486 TACGTA TAC^GTA
2528 TACGTA
SphI 2780 GCATGC GCATG^C
3160 GCATGC
SspBI 2438 TGTACA T^GTACA
2473 TGTACA
SspI 24 AATATT AAT^ATT
1717 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoI XhoII