################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AclI AgeI Alw44I ApaLI AscI Asp718I AviII AvrII BbrPI BciVI BglI BlnI BsaBI BsiEI BsiWI BsmBI BsrFI BssHII BstAPI Cfr10I CpoI DraIII DrdI EagI EclXI Eco52I EcoT22I FseI FspI KpnI MamI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmaCI PmlI Psp1406I PvuI RsrII SbfI SexAI SfiI SgrAI SpeI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 606 GACGTC GACGT^C AflII 563 CTTAAG C^TTAAG AflIII 4697 ACATGT A^C[GA][CT]GT AhdI 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3895 AGCGCT AGC^GCT Asp700I 2414 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 111 TGGCCA TGG^CCA BbeI 3177 GGCGCC GGCGC^C BfrI 563 CTTAAG C^TTAAG BglII 559 AGATCT A^GATCT BspLU11I 4697 ACATGT A^CATGT BsrBI 2745 GAGCGG CCGCTC(-3/-3) BssSI 466 CACGAG CACGAG(-5/-1) Bst1107I 1262 GTATAC GTA^TAC BstBI 578 TTCGAA TT^CGAA BstEII 1280 GGTCACC G^GT[ACGT]ACC BstPI 1280 GGTCACC G^GT[ACGT]ACC BstZ17I 1262 GTATAC GTA^TAC Eam1105I 2247 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3895 AGCGCT AGC^GCT EcoNI 3181 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1280 GGTCACC G^GT[ACGT]ACC EcoRI 3903 GAATTC G^AATTC HpaI 2382 GTTAAC GTT^AAC KasI 3177 GGCGCC G^GCGCC KspI 2575 CCGCGG CCGC^GG MluNI 111 TGGCCA TGG^CCA MscI 111 TGGCCA TGG^CCA NarI 3177 GGCGCC GG^CGCC NcoI 4210 CCATGG C^CATGG NspV 578 TTCGAA TT^CGAA PflFI 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 103 CCATTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 1406 GTTTAAAC GTTT^AAAC PshAI 1024 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 2575 CCGCGG CCGC^GG SalI 3405 GTCGAC G^TCGAC ScaI 4057 AGTACT AGT^ACT SfoI 3177 GGCGCC GGC^GCC SfuI 578 TTCGAA TT^CGAA SmaI 1017 CCCGGG CCC^GGG Tth111I 2711 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 103 CCATTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1017 CCCGGG C^CCGGG XmaI 1017 CCCGGG C^CCGGG XmnI 2414 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 45 enzymes) # ################################### AccI 1262 GTATAC GT^[AC][GT]AC 3405 GTCGAC AccII 1421 CGCG CG^CG 2576 CGCG AccIII 1910 TCCGGA T^CCGGA 4564 TCCGGA AocI 1191 CCTGAGG CC^T[ACGT]AGG 4539 CCTCAGG ApaI 3085 GGGCCC GGGCC^C 4510 GGGCCC AseI 105 ATTAAT AT^TAAT 2331 ATTAAT AsnI 105 ATTAAT AT^TAAT 2331 ATTAAT BamHI 1216 GGATCC G^GATCC 3332 GGATCC BanI 3177 GGCGCC G^G[CT][GA]CC 4239 GGTGCC BbsI 528 GAAGAC GAAGAC(2/6) 3954 GAAGAC BbvCI 597 GCTGAGG CCTCAGC(-5/-2) 653 GCTGAGG BcgI 1041 CGACGGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3521 CGAGAGGGCTGC BclI 1600 TGATCA T^GATCA 1625 TGATCA BlpI 2292 GCTAAGC GC^T[ACGT]AGC 3531 GCTCAGC Bpu1102I 2292 GCTAAGC GC^T[ACGT]AGC 3531 GCTCAGC BpuAI 528 GAAGAC GAAGAC(2/6) 3954 GAAGAC BsaWI 1910 TCCGGA [AT]^CCGG[AT] 4564 TCCGGA BseAI 1910 TCCGGA T^CCGGA 4564 TCCGGA BsgI 912 CTGCAC GTGCAG(16/14) 2206 CTGCAC Bsp1407I 2438 TGTACA T^GTACA 2473 TGTACA BspDI 436 ATCGAT AT^CGAT 1636 ATCGAT BspEI 1910 TCCGGA T^CCGGA 4564 TCCGGA BspHI 338 TCATGA T^CATGA 2822 TCATGA BsrGI 2438 TGTACA T^GTACA 2473 TGTACA BstUI 1421 CGCG CG^CG 2576 CGCG Bsu36I 1191 CCTGAGG CC^T[ACGT]AGG 4539 CCTCAGG CelII 2292 GCTAAGC GC^T[ACGT]AGC 3531 GCTCAGC ClaI 436 ATCGAT AT^CGAT 1636 ATCGAT EaeI 111 TGGCCA [CT]^GGCC[GA] 4469 TGGCCG Eco81I 1191 CCTGAGG CC^T[ACGT]AGG 4539 CCTCAGG EcoRV 3776 GATATC GAT^ATC 4191 GATATC FbaI 1600 TGATCA T^GATCA 1625 TGATCA HgaI 3750 GACGC GACGC(5/10) 3403 GCGTC HindIII 168 AAGCTT A^AGCTT 4559 AAGCTT MroI 1910 TCCGGA T^CCGGA 4564 TCCGGA MvnI 1421 CGCG CG^CG 2576 CGCG NdeI 442 CATATG CA^TATG 646 CATATG PpuMI 671 GGGTCCT [GA]G^G[AT]CC[CT] 3070 AGGACCT PshBI 105 ATTAAT AT^TAAT 2331 ATTAAT RcaI 338 TCATGA T^CATGA 2822 TCATGA SapI 978 GAAGAGC GCTCTTC(1/4) 3536 GCTCTTC SnaBI 486 TACGTA TAC^GTA 2528 TACGTA SphI 2780 GCATGC GCATG^C 3160 GCATGC SspBI 2438 TGTACA T^GTACA 2473 TGTACA SspI 24 AATATT AAT^ATT 1717 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII