##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI BbeI BcgI BfrI
BsaBI BsaHI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FseI Hin1I HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 3412 GTCTAC GT^[AC][GT]AC
AccII 2892 CGCG CG^CG
AccIII 1013 TCCGGA T^CCGGA
AcsI 1835 GAATTC [GA]^AATT[CT]
AflIII 3859 ACACGT A^C[GA][CT]GT
Alw44I 3350 GTGCAC G^TGCAC
AocI 1311 CCTCAGG CC^T[ACGT]AGG
Aor51HI 103 AGCGCT AGC^GCT
ApaI 823 GGGCCC GGGCC^C
ApaLI 3350 GTGCAC G^TGCAC
ApoI 1835 GAATTC [GA]^AATT[CT]
AvaI 2920 CCCGAG C^[CT]CG[GA]G
BamHI 3741 GGATCC G^GATCC
BbrPI 2937 CACGTG CAC^GTG
BbvCI 2493 CCTCAGC CCTCAGC(-5/-2)
BciVI 3853 GTATCC GTATCC(6/5)
BclI 1595 TGATCA T^GATCA
BglI 3912 GCCTAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1461 AGATCT A^GATCT
BlpI 446 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 446 GCTGAGC GC^T[ACGT]AGC
BsaAI 2937 CACGTG [CT]AC^GT[GA]
BseAI 1013 TCCGGA T^CCGGA
BsmI 1951 GAATGC GAATGC(1/-1)
BsoBI 2920 CCCGAG C^[CT]CG[GA]G
BspEI 1013 TCCGGA T^CCGGA
BstBI 54 TTCGAA TT^CGAA
BstUI 2892 CGCG CG^CG
BstXI 2875 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1311 CCTCAGG CC^T[ACGT]AGG
CelII 446 GCTGAGC GC^T[ACGT]AGC
Eco47III 103 AGCGCT AGC^GCT
Eco81I 1311 CCTCAGG CC^T[ACGT]AGG
EcoNI 2474 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1835 GAATTC G^AATTC
FbaI 1595 TGATCA T^GATCA
HindIII 3792 AAGCTT A^AGCTT
MroI 1013 TCCGGA T^CCGGA
MseI 2624 TTAA T^TAA
MslI 2201 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 2892 CGCG CG^CG
NspI 3108 GCATGC [GA]CATG^[CT]
NspV 54 TTCGAA TT^CGAA
PmaCI 2937 CACGTG CAC^GTG
PmlI 2937 CACGTG CAC^GTG
PshAI 1366 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3875 GAGCTC GAGCT^C
SfiI 3911 GGCCTAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 54 TTCGAA TT^CGAA
SphI 3108 GCATGC GCATG^C
SspI 2617 AATATT AAT^ATT
TfiI 1227 GATTC G^A[AT]TC
Tru9I 2624 TTAA T^TAA
XcmI 2539 CCAGATGCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Asp700I 5 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
478 GAAAGATTTC
AspI 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCCAGTC
AviII 1195 TGCGCA TGC^GCA
1456 TGCGCA
AvrII 595 CCTAGG C^CTAGG
3943 CCTAGG
BanI 2188 GGCACC G^G[CT][GA]CC
2728 GGCACC
BlnI 595 CCTAGG C^CTAGG
3943 CCTAGG
BsaWI 1013 TCCGGA [AT]^CCGG[AT]
1338 ACCGGA
BspMI 2480 GCAGGT ACCTGC(4/8)
2772 GCAGGT
BssSI 406 CTCGTG CACGAG(-5/-1)
3878 CTCGTG
DraIII 1480 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3025 CACTGTGTG
FspI 1195 TGCGCA TGC^GCA
1456 TGCGCA
HaeII 103 AGCGCT [GA]GCGC^[CT]
285 AGCGCC
HincII 3281 GTCAAC GT[CT]^[GA]AC
3674 GTCAAC
HindII 3281 GTCAAC GT[CT]^[GA]AC
3674 GTCAAC
NdeI 3700 CATATG CA^TATG
3894 CATATG
PflFI 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCCAGTC
PpuMI 2442 AGGACCT [GA]G^G[AT]CC[CT]
3040 AGGTCCT
SapI 1244 GCTCTTC GCTCTTC(1/4)
3152 GCTCTTC
ScaI 1434 AGTACT AGT^ACT
1633 AGTACT
SmlI 260 CTCAAG C^T[CT][GA]AG
332 CTCAAG
StuI 31 AGGCCT AGG^CCT
275 AGGCCT
Tth111I 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2811 GACCCAGTC
XmnI 5 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
478 GAAAGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XhoII