################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI BbeI BcgI BfrI BsaBI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 3412 GTCTAC GT^[AC][GT]AC AccII 2892 CGCG CG^CG AccIII 1013 TCCGGA T^CCGGA AcsI 1835 GAATTC [GA]^AATT[CT] AflIII 3859 ACACGT A^C[GA][CT]GT Alw44I 3350 GTGCAC G^TGCAC AocI 1311 CCTCAGG CC^T[ACGT]AGG Aor51HI 103 AGCGCT AGC^GCT ApaI 823 GGGCCC GGGCC^C ApaLI 3350 GTGCAC G^TGCAC ApoI 1835 GAATTC [GA]^AATT[CT] AvaI 2920 CCCGAG C^[CT]CG[GA]G BamHI 3741 GGATCC G^GATCC BbrPI 2937 CACGTG CAC^GTG BbvCI 2493 CCTCAGC CCTCAGC(-5/-2) BciVI 3853 GTATCC GTATCC(6/5) BclI 1595 TGATCA T^GATCA BglI 3912 GCCTAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1461 AGATCT A^GATCT BlpI 446 GCTGAGC GC^T[ACGT]AGC Bpu1102I 446 GCTGAGC GC^T[ACGT]AGC BsaAI 2937 CACGTG [CT]AC^GT[GA] BseAI 1013 TCCGGA T^CCGGA BsmI 1951 GAATGC GAATGC(1/-1) BsoBI 2920 CCCGAG C^[CT]CG[GA]G BspEI 1013 TCCGGA T^CCGGA BstBI 54 TTCGAA TT^CGAA BstUI 2892 CGCG CG^CG BstXI 2875 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1311 CCTCAGG CC^T[ACGT]AGG CelII 446 GCTGAGC GC^T[ACGT]AGC Eco47III 103 AGCGCT AGC^GCT Eco81I 1311 CCTCAGG CC^T[ACGT]AGG EcoNI 2474 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1835 GAATTC G^AATTC FbaI 1595 TGATCA T^GATCA HindIII 3792 AAGCTT A^AGCTT MroI 1013 TCCGGA T^CCGGA MseI 2624 TTAA T^TAA MslI 2201 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 2892 CGCG CG^CG NspI 3108 GCATGC [GA]CATG^[CT] NspV 54 TTCGAA TT^CGAA PmaCI 2937 CACGTG CAC^GTG PmlI 2937 CACGTG CAC^GTG PshAI 1366 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3875 GAGCTC GAGCT^C SfiI 3911 GGCCTAGTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 54 TTCGAA TT^CGAA SphI 3108 GCATGC GCATG^C SspI 2617 AATATT AAT^ATT TfiI 1227 GATTC G^A[AT]TC Tru9I 2624 TTAA T^TAA XcmI 2539 CCAGATGCCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### Asp700I 5 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 478 GAAAGATTTC AspI 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACCCAGTC AviII 1195 TGCGCA TGC^GCA 1456 TGCGCA AvrII 595 CCTAGG C^CTAGG 3943 CCTAGG BanI 2188 GGCACC G^G[CT][GA]CC 2728 GGCACC BlnI 595 CCTAGG C^CTAGG 3943 CCTAGG BsaWI 1013 TCCGGA [AT]^CCGG[AT] 1338 ACCGGA BspMI 2480 GCAGGT ACCTGC(4/8) 2772 GCAGGT BssSI 406 CTCGTG CACGAG(-5/-1) 3878 CTCGTG DraIII 1480 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 3025 CACTGTGTG FspI 1195 TGCGCA TGC^GCA 1456 TGCGCA HaeII 103 AGCGCT [GA]GCGC^[CT] 285 AGCGCC HincII 3281 GTCAAC GT[CT]^[GA]AC 3674 GTCAAC HindII 3281 GTCAAC GT[CT]^[GA]AC 3674 GTCAAC NdeI 3700 CATATG CA^TATG 3894 CATATG PflFI 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACCCAGTC PpuMI 2442 AGGACCT [GA]G^G[AT]CC[CT] 3040 AGGTCCT SapI 1244 GCTCTTC GCTCTTC(1/4) 3152 GCTCTTC ScaI 1434 AGTACT AGT^ACT 1633 AGTACT SmlI 260 CTCAAG C^T[CT][GA]AG 332 CTCAAG StuI 31 AGGCCT AGG^CCT 275 AGGCCT Tth111I 2226 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2811 GACCCAGTC XmnI 5 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 478 GAAAGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII