##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI Alw44I AocI
ApaLI AscI Asp718I AviII BcgI
BciVI BsiWI BspDI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DrdI Eco81I EcoO65I EcoT22I
FseI FspI HpaI KpnI KspI
MluI NaeI NgoMIV NotI NruI
NsiI NspV PacI PshAI Psp1406I
PvuI SacII SapI SexAI SfiI
SfuI SnaBI SpeI StuI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AgeI 2627 ACCGGT A^CCGGT
AhdI 1836 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2874 GGGCCC GGGCC^C
AseI 3479 ATTAAT AT^TAAT
AsnI 3479 ATTAAT AT^TAAT
Asp700I 2070 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1836 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1248 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 62 CCTAGG C^CTAGG
BamHI 1109 GGATCC G^GATCC
BbeI 309 GGCGCC GGCGC^C
BglI 3854 GCCGTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 385 AGATCT A^GATCT
BlnI 62 CCTAGG C^CTAGG
BsaBI 1104 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2012 GGTCTC GGTCTC(1/5)
Bsp1407I 1573 TGTACA T^GTACA
BspHI 1079 TCATGA T^CATGA
BspLU11I 3794 ACATGT A^CATGT
BspMI 2996 ACCTGC ACCTGC(4/8)
BsrGI 1573 TGTACA T^GTACA
BssHII 57 GCGCGC G^CGCGC
BssSI 2900 CTCGTG CACGAG(-5/-1)
CpoI 29 CGGTCCG CG^G[AT]CCG
DraIII 3668 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1836 GACCTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 936 GAAGAG CTCTTC(1/4)
EcoRI 2025 GAATTC G^AATTC
EcoRV 2548 GATATC GAT^ATC
HindIII 464 AAGCTT A^AGCTT
KasI 309 GGCGCC G^GCGCC
Ksp632I 936 GAAGAG CTCTTC(1/4)
MamI 1104 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 309 GGCGCC GG^CGCC
NdeI 2599 CATATG CA^TATG
PaeR7I 2809 CTCGAG C^TCGAG
PflFI 1248 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2627 ACCGGT A^CCGGT
PmeI 4077 GTTTAAAC GTTT^AAAC
PshBI 3479 ATTAAT AT^TAAT
RcaI 1079 TCATGA T^CATGA
RsrII 29 CGGTCCG CG^G[AT]CCG
SalI 1419 GTCGAC G^TCGAC
SbfI 2451 CCTGCAGG CCTGCA^GG
SfoI 309 GGCGCC GGC^GCC
SgrAI 2626 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2553 CCCGGG CCC^GGG
Sse8387I 2451 CCTGCAGG CCTGCA^GG
SspBI 1573 TGTACA T^GTACA
Tth111I 1248 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 581 CCAGTTTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2809 CTCGAG C^TCGAG
XmaCI 2553 CCCGGG C^CCGGG
XmaI 2553 CCCGGG C^CCGGG
XmnI 2070 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccII 58 CGCG CG^CG
102 CGCG
AccIII 1091 TCCGGA T^CCGGA
3806 TCCGGA
AcyI 125 GGCGTC G[GA]^CG[CT]C
309 GGCGCC
AflIII 3794 ACATGT A^C[GA][CT]GT
4083 ACGTGT
Aor51HI 1654 AGCGCT AGC^GCT
2274 AGCGCT
BbrPI 401 CACGTG CAC^GTG
2739 CACGTG
BbsI 152 GAAGAC GAAGAC(2/6)
2216 GAAGAC
BbvCI 2694 CCTCAGC CCTCAGC(-5/-2)
326 GCTGAGG
BclI 338 TGATCA T^GATCA
970 TGATCA
BpuAI 152 GAAGAC GAAGAC(2/6)
2216 GAAGAC
BsaAI 401 CACGTG [CT]AC^GT[GA]
2739 CACGTG
BsaHI 125 GGCGTC G[GA]^CG[CT]C
309 GGCGCC
BseAI 1091 TCCGGA T^CCGGA
3806 TCCGGA
BsmBI 318 GAGACG CGTCTC(1/5)
2227 GAGACG
BsmI 1712 GAATGC GAATGC(1/-1)
4385 GAATGC
BspEI 1091 TCCGGA T^CCGGA
3806 TCCGGA
BsrBI 33 CCGCTC CCGCTC(-3/-3)
212 GAGCGG
BsrFI 46 GCCGGT [GA]^CCGG[CT]
2627 ACCGGT
BstUI 58 CGCG CG^CG
102 CGCG
BstXI 175 CCAAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2572 CCATGCCCATGG
Cfr10I 46 GCCGGT [GA]^CCGG[CT]
2627 ACCGGT
EagI 107 CGGCCG C^GGCCG
247 CGGCCG
EclXI 107 CGGCCG C^GGCCG
247 CGGCCG
Eco47III 1654 AGCGCT AGC^GCT
2274 AGCGCT
Eco52I 107 CGGCCG C^GGCCG
247 CGGCCG
FbaI 338 TGATCA T^GATCA
970 TGATCA
Hin1I 125 GGCGTC G[GA]^CG[CT]C
309 GGCGCC
MroI 1091 TCCGGA T^CCGGA
3806 TCCGGA
MvnI 58 CGCG CG^CG
102 CGCG
NcoI 121 CCATGG C^CATGG
2578 CCATGG
NheI 513 GCTAGC G^CTAGC
2933 GCTAGC
PflMI 2795 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3546 CCATAATTTGG
PmaCI 401 CACGTG CAC^GTG
2739 CACGTG
PmlI 401 CACGTG CAC^GTG
2739 CACGTG
PpuMI 2666 AGGACCC [GA]G^G[AT]CC[CT]
3877 AGGTCCC
SacI 255 GAGCTC GAGCT^C
2232 GAGCTC
ScaI 235 AGTACT AGT^ACT
3061 AGTACT
SphI 2324 GCATGC GCATG^C
3773 GCATGC
SspI 1469 AATATT AAT^ATT
3528 AATATT
Van91I 2795 CCACGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3546 CCATAATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII