##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I AocI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbrPI
BclI BlnI BsaBI BseAI BsiWI
BspDI BspEI BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI Eam1105I Eco81I
EcoNI FbaI FseI FspI HindIII
KpnI KspI MamI MluI MroI
NdeI NheI NruI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3099 GACGTC GACGT^C
AflIII 4381 ACATGT A^C[GA][CT]GT
Aor51HI 2013 AGCGCT AGC^GCT
ApaI 1929 GGGCCC GGGCC^C
BcgI 3166 GCAGCTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4265 GGATAC GTATCC(6/5)
BglI 1730 GCCTTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2548 AGATCT A^GATCT
BlpI 1898 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1898 GCTGAGC GC^T[ACGT]AGC
BsaAI 2027 TACGTG [CT]AC^GT[GA]
BsaWI 3213 ACCGGA [AT]^CCGG[AT]
BsgI 4362 CTGCAC GTGCAG(16/14)
BsmI 1423 GAATGC GAATGC(1/-1)
BspHI 3892 TCATGA T^CATGA
BspLU11I 4381 ACATGT A^CATGT
BssHII 222 GCGCGC G^CGCGC
CelII 1898 GCTGAGC GC^T[ACGT]AGC
CpoI 261 CGGTCCG CG^G[AT]CCG
DraI 4475 TTTAAA TTT^AAA
DraIII 1001 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1515 CCATGG C^C[GA][CT]GG
Eco47III 2013 AGCGCT AGC^GCT
EcoRI 3315 GAATTC G^AATTC
EcoRV 4139 GATATC GAT^ATC
EcoT22I 3887 ATGCAT ATGCA^T
HincII 1814 GTTAAC GT[CT]^[GA]AC
HindII 1814 GTTAAC GT[CT]^[GA]AC
HpaI 1814 GTTAAC GTT^AAC
MfeI 745 CAATTG C^AATTG
MunI 745 CAATTG C^AATTG
NaeI 247 GCCGGC GCC^GGC
NcoI 1515 CCATGG C^CATGG
NgoMIV 247 GCCGGC G^CCGGC
NsiI 3887 ATGCAT ATGCA^T
PaeR7I 399 CTCGAG C^TCGAG
PflMI 2924 CCATTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3892 TCATGA T^CATGA
RsrII 261 CGGTCCG CG^G[AT]CCG
SapI 3050 GAAGAGC GCTCTTC(1/4)
SmaI 30 CCCGGG CCC^GGG
Van91I 2924 CCATTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3386 TCTAGA T^CTAGA
XhoI 399 CTCGAG C^TCGAG
XmaCI 30 CCCGGG C^CCGGG
XmaI 30 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 2552 CTTAAG C^TTAAG
2887 CTTAAG
AseI 1834 ATTAAT AT^TAAT
4076 ATTAAT
AsnI 1834 ATTAAT AT^TAAT
4076 ATTAAT
Asp700I 3122 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3489 GAAGCAATTC
AvaI 30 CCCGGG C^[CT]CG[GA]G
399 CTCGAG
BfrI 2552 CTTAAG C^TTAAG
2887 CTTAAG
BsaI 404 GAGACC GGTCTC(1/5)
2162 GAGACC
BsmBI 3001 CGTCTC CGTCTC(1/5)
1046 GAGACG
BsoBI 30 CCCGGG C^[CT]CG[GA]G
399 CTCGAG
Bsp1407I 567 TGTACA T^GTACA
3394 TGTACA
BsrBI 149 GAGCGG CCGCTC(-3/-3)
1901 GAGCGG
BsrFI 247 GCCGGC [GA]^CCGG[CT]
3627 ACCGGC
BsrGI 567 TGTACA T^GTACA
3394 TGTACA
BstAPI 1307 GCAATGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1373 GCAGATCGTGC
BstXI 954 CCAGGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1710 CCATCAGAATGG
Cfr10I 247 GCCGGC [GA]^CCGG[CT]
3627 ACCGGC
DrdI 3093 GACGTGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4809 GACAGCTAAGTC
NotI 127 GCGGCCGC GC^GGCCGC
171 GCGGCCGC
NspI 598 ACATGC [GA]CATG^[CT]
4381 ACATGT
PshBI 1834 ATTAAT AT^TAAT
4076 ATTAAT
SfcI 771 CTGTAG C^T[GA][CT]AG
3914 CTACAG
SspBI 567 TGTACA T^GTACA
3394 TGTACA
XmnI 3122 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3489 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII