##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BglI
BlnI BsaAI BsaI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
FspI HaeII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI Tth111I Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1562 GTATAC GT^[AC][GT]AC
AccII 1 CGCG CG^CG
AcyI 500 GGCGTC G[GA]^CG[CT]C
AlwNI 2402 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 1156 CTCGGG C^[CT]CG[GA]G
BamHI 2234 GGATCC G^GATCC
BanI 1264 GGCACC G^G[CT][GA]CC
BclI 2803 TGATCA T^GATCA
BcnI 394 CCCGG CC^[GC]GG
BlpI 1066 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1066 GCTGAGC GC^T[ACGT]AGC
BsaHI 500 GGCGTC G[GA]^CG[CT]C
BseRI 775 CTCCTC GAGGAG(10/8)
BsmBI 853 CGTCTC CGTCTC(1/5)
BsmFI 2961 GTCCC GGGAC(10/14)
BsmI 1100 GAATGC GAATGC(1/-1)
BsoBI 1156 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1766 TGTACA T^GTACA
BsrGI 1766 TGTACA T^GTACA
Bst1107I 1562 GTATAC GTA^TAC
BstUI 1 CGCG CG^CG
BstZ17I 1562 GTATAC GTA^TAC
CelII 1066 GCTGAGC GC^T[ACGT]AGC
DraII 941 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 862 CCATGG C^C[GA][CT]GG
EcoNI 1250 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 941 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 2938 GAATTC G^AATTC
FbaI 2803 TGATCA T^GATCA
Hin1I 500 GGCGTC G[GA]^CG[CT]C
HindIII 2721 AAGCTT A^AGCTT
MaeII 2680 ACGT A^CGT
MvnI 1 CGCG CG^CG
NciI 394 CCCGG CC^[GC]GG
NcoI 862 CCATGG C^CATGG
PpuMI 941 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 2996 CAGCTG CAG^CTG
SexAI 1838 ACCAGGT A^CC[AT]GGT
SphI 1079 GCATGC GCATG^C
SspBI 1766 TGTACA T^GTACA
SwaI 3164 ATTTAAAT ATTT^AAAT
XbaI 1635 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflII 670 CTTAAG C^TTAAG
2724 CTTAAG
AflIII 1558 ACATGT A^C[GA][CT]GT
1873 ACATGT
AvaII 22 GGTCC G^G[AT]CC
942 GGACC
BciVI 788 GTATCC GTATCC(6/5)
3158 GTATCC
BfrI 670 CTTAAG C^TTAAG
2724 CTTAAG
BglII 1046 AGATCT A^GATCT
2469 AGATCT
BpmI 2050 CTCCAG CTGGAG(16/14)
293 CTGGAG
BsaBI 921 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1627 GATTTCTATC
BsmAI 854 GTCTC GTCTC(1/5)
2388 GTCTC
BspLU11I 1558 ACATGT A^CATGT
1873 ACATGT
BsrDI 627 CATTGC GCAATG(2/0)
2786 CATTGC
CfoI 2 GCGC GCG^C
1108 GCGC
Cfr13I 22 GGTCC G^G[ACGT]CC
942 GGACC
EcoT14I 862 CCATGG C^C[AT][AT]GG
2221 CCTTGG
HapII 51 CCGG C^CGG
395 CCGG
HgaI 501 GCGTC GACGC(5/10)
2844 GCGTC
HhaI 2 GCGC GCG^C
1108 GCGC
HinP1I 2 GCGC G^CGC
1108 GCGC
HpaI 1752 GTTAAC GTT^AAC
1834 GTTAAC
HpaII 51 CCGG C^CGG
395 CCGG
MamI 921 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1627 GATTTCTATC
MspA1I 1146 CAGCGG C[AC]G^C[GT]G
2996 CAGCTG
MspI 51 CCGG C^CGG
395 CCGG
SapI 352 GAAGAGC GCTCTTC(1/4)
697 GAAGAGC
Sau96I 22 GGTCC G^G[ACGT]CC
942 GGACC
SspI 613 AATATT AAT^ATT
3008 AATATT
StyI 862 CCATGG C^C[AT][AT]GG
2221 CCTTGG
TaqI 194 TCGA T^CGA
337 TCGA
TthHB8I 194 TCGA T^CGA
337 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
DraI EarI EcoRII FauI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
SacI Sau3AI ScaI ScrFI SfaNI
SfcI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI