##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BbeI
BbvCI BcgI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsmBI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrFI BsrGI BssHII
BssKI BssSI Bst1107I BstBI BstEII
BstNI BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRII FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HphI KasI KpnI Ksp632I
KspI MflI MluI MroI MvaI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI Sau96I
SbfI ScaI ScrFI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 67 enzymes) #
###################################
AciI 126 GCGG CCGC(-3/-1)
AfaI 426 GTAC GT^AC
AlwI 312 GGATC GGATC(4/5)
AlwNI 253 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1113 ATTAAT AT^TAAT
AsnI 1113 ATTAAT AT^TAAT
AspI 741 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 342 TGGCCA TGG^CCA
BanI 132 GGTGCC G^G[CT][GA]CC
BanII 162 GAGCTC G[GA]GC[CT]^C
BbrPI 144 CACGTG CAC^GTG
BbsI 169 GAAGAC GAAGAC(2/6)
BciVI 672 GGATAC GTATCC(6/5)
BclI 713 TGATCA T^GATCA
BpuAI 169 GAAGAC GAAGAC(2/6)
BsaAI 144 CACGTG [CT]AC^GT[GA]
BsaBI 1011 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 368 GAGACC GGTCTC(1/5)
BsaWI 371 ACCGGA [AT]^CCGG[AT]
BseRI 638 GAGGAG GAGGAG(10/8)
BsrBI 124 GAGCGG CCGCTC(-3/-3)
BsrDI 878 GCAATG GCAATG(2/0)
BsrI 205 CCAGT ACTGG(1/-1)
BstAPI 252 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 357 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 441 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 911 GACTAATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 345 CCATGG C^C[GA][CT]GG
EaeI 342 TGGCCA [CT]^GGCC[GA]
EcoNI 623 CCTGTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1153 GATATC GAT^ATC
EcoT22I 1012 ATGCAT ATGCA^T
FbaI 713 TGATCA T^GATCA
HaeIII 343 GGCC GG^CC
HapII 372 CCGG C^CGG
HgaI 571 GCGTC GACGC(5/10)
HincII 238 GTTAAC GT[CT]^[GA]AC
HindII 238 GTTAAC GT[CT]^[GA]AC
HindIII 787 AAGCTT A^AGCTT
HpaI 238 GTTAAC GTT^AAC
HpaII 372 CCGG C^CGG
MaeII 145 ACGT A^CGT
MamI 1011 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 404 CAATTG C^AATTG
MluNI 342 TGGCCA TGG^CCA
MscI 342 TGGCCA TGG^CCA
MslI 58 CACATGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 32 CAGCTG C[AC]G^C[GT]G
MspI 372 CCGG C^CGG
MunI 404 CAATTG C^AATTG
NcoI 345 CCATGG C^CATGG
NsiI 1012 ATGCAT ATGCA^T
PflFI 741 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 741 GACTC GAGTC(4/5)
PmaCI 144 CACGTG CAC^GTG
PmlI 144 CACGTG CAC^GTG
PshBI 1113 ATTAAT AT^TAAT
PvuII 32 CAGCTG CAG^CTG
RsaI 426 GTAC GT^AC
SacI 162 GAGCTC GAGCT^C
SfaNI 1011 GATGC GCATC(5/9)
SmlI 189 CTCAAG C^T[CT][GA]AG
SspI 1276 AATATT AAT^ATT
TfiI 417 GATTC G^A[AT]TC
Tsp45I 909 GTGAC ^GT[GC]AC
Tth111I 741 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 580 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 851 AAATTT [GA]^AATT[CT]
1090 GAATTT
ApoI 851 AAATTT [GA]^AATT[CT]
1090 GAATTT
BsaJI 345 CCATGG C^C[ACGT][ACGT]GG
1304 CCTTGG
BsgI 103 GTGCAG GTGCAG(16/14)
250 GTGCAG
BsiYI 499 CCTATTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
623 CCTGTCTAAGG
BslI 499 CCTATTATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
623 CCTGTCTAAGG
BsmAI 368 GAGAC GTCTC(1/5)
805 GAGAC
BsmI 382 GAATGC GAATGC(1/-1)
647 GAATGC
BstF5I 487 CATCC GGATG(2/0)
374 GGATG
Cac8I 82 GCCAGC GC[ACGT]^[ACGT]GC
356 GCCAGC
DpnII 313 GATC ^GATC
714 GATC
EcoT14I 345 CCATGG C^C[AT][AT]GG
1304 CCTTGG
FokI 487 CATCC GGATG(9/13)
374 GGATG
HinfI 417 GATTC G^A[ACGT]TC
741 GACTC
MaeIII 692 GTAAC ^GT[ACGT]AC
909 GTGAC
MboI 313 GATC ^GATC
714 GATC
NdeII 313 GATC ^GATC
714 GATC
NlaIV 132 GGTGCC GG[ACGT]^[ACGT]CC
619 GGAGCC
PstI 211 CTGCAG CTGCA^G
259 CTGCAG
Sau3AI 313 GATC ^GATC
714 GATC
StyI 345 CCATGG C^C[AT][AT]GG
1304 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AflIII AluI AspHI BbvI BfaI
BmyI BsiHKAI Bsp1286I BspLU11I DdeI
DraI Fnu4HI ItaI MaeI MboII
MnlI MseI MwoI NlaIII NspI
SfcI Tru9I TseI Tsp509I TspRI