##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccI AclI AgeI Aor51HI AscI
Asp700I AspI BbrPI BcgI BglII
BsaAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BsrDI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I HpaI MamI
MfeI MluI MunI NdeI NheI
NotI NsiI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SapI SfiI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3739 GACGTC GACGT^C
AflII 544 CTTAAG C^TTAAG
AflIII 3243 ACATGT A^C[GA][CT]GT
Alw44I 3801 GTGCAC G^TGCAC
ApaLI 3801 GTGCAC G^TGCAC
AseI 4863 ATTAAT AT^TAAT
AsnI 4863 ATTAAT AT^TAAT
AviII 739 TGCGCA TGC^GCA
AvrII 1029 CCTAGG C^CTAGG
BbeI 4406 GGCGCC GGCGC^C
BclI 3750 TGATCA T^GATCA
BfrI 544 CTTAAG C^TTAAG
BglI 4664 GCCCAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1029 CCTAGG C^CTAGG
BsmBI 3737 GAGACG CGTCTC(1/5)
BspHI 3690 TCATGA T^CATGA
BspLU11I 3243 ACATGT A^CATGT
BspMI 1074 GCAGGT ACCTGC(4/8)
BsrFI 3776 GCCGGC [GA]^CCGG[CT]
BssSI 4268 CTCGTG CACGAG(-5/-1)
BstBI 147 TTCGAA TT^CGAA
Cfr10I 3776 GCCGGC [GA]^CCGG[CT]
DrdI 27 GACCCGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2605 GATATC GAT^ATC
FbaI 3750 TGATCA T^GATCA
FseI 3775 GGCCGGCC GGCCGG^CC
FspI 739 TGCGCA TGC^GCA
HincII 3852 GTCAAC GT[CT]^[GA]AC
HindII 3852 GTCAAC GT[CT]^[GA]AC
KasI 4406 GGCGCC G^GCGCC
KspI 1756 CCGCGG CCGC^GG
NaeI 3776 GCCGGC GCC^GGC
NarI 4406 GGCGCC GG^CGCC
NgoMIV 3776 GCCGGC G^CCGGC
NruI 999 TCGCGA TCG^CGA
NspV 147 TTCGAA TT^CGAA
PacI 4860 TTAATTAA TTAAT^TAA
PshAI 4777 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4863 ATTAAT AT^TAAT
RcaI 3690 TCATGA T^CATGA
SacII 1756 CCGCGG CCGC^GG
ScaI 1587 AGTACT AGT^ACT
SexAI 4740 ACCAGGT A^CC[AT]GGT
SfoI 4406 GGCGCC GGC^GCC
SfuI 147 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 2625 GGTACC G^GTACC
4367 GGTACC
AcsI 2513 AAATTC [GA]^AATT[CT]
2570 AAATTC
AcyI 3739 GACGTC G[GA]^CG[CT]C
4406 GGCGCC
AhdI 541 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3161 GACCTCGAGTC
ApoI 2513 AAATTC [GA]^AATT[CT]
2570 AAATTC
Asp718I 2625 GGTACC G^GTACC
4367 GGTACC
AspEI 541 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3161 GACCTCGAGTC
BamHI 493 GGATCC G^GATCC
1850 GGATCC
BbvCI 1737 CCTCAGC CCTCAGC(-5/-2)
4495 CCTCAGC
BciVI 1456 GTATCC GTATCC(6/5)
3104 GTATCC
BsaHI 3739 GACGTC G[GA]^CG[CT]C
4406 GGCGCC
BsaI 25 GAGACC GGTCTC(1/5)
282 GAGACC
BsmI 3041 GAATGC GAATGC(1/-1)
2522 GCATTC
DraIII 1281 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4739 CACCAGGTG
Eam1105I 541 GACCTTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3161 GACCTCGAGTC
Hin1I 3739 GACGTC G[GA]^CG[CT]C
4406 GGCGCC
HindIII 2105 AAGCTT A^AGCTT
4720 AAGCTT
KpnI 2625 GGTACC GGTAC^C
4367 GGTACC
NcoI 782 CCATGG C^CATGG
4235 CCATGG
SbfI 1521 CCTGCAGG CCTGCA^GG
2718 CCTGCAGG
SphI 1509 GCATGC GCATG^C
2148 GCATGC
Sse8387I 1521 CCTGCAGG CCTGCA^GG
2718 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII XmaCI XmaI