##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AccI AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BfrI BglI BglII BlnI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrFI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HpaI KasI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NotI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI PvuI
RsrII SacII SalI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 3162 GACGTC GACGT^C
AccII 4110 CGCG CG^CG
AclI 2424 AACGTT AA^CGTT
AcyI 3162 GACGTC G[GA]^CG[CT]C
Asp700I 1000 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2954 CACGTG CAC^GTG
BcgI 3608 CGATTACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2718 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2718 GCTGAGC GC^T[ACGT]AGC
BsaAI 2954 CACGTG [CT]AC^GT[GA]
BsaHI 3162 GACGTC G[GA]^CG[CT]C
BspHI 2131 TCATGA T^CATGA
BspLU11I 4036 ACATGT A^CATGT
BstAPI 1289 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 4110 CGCG CG^CG
CelII 2718 GCTGAGC GC^T[ACGT]AGC
DraI 734 TTTAAA TTT^AAA
EcoT22I 951 ATGCAT ATGCA^T
Hin1I 3162 GACGTC G[GA]^CG[CT]C
MfeI 162 CAATTG C^AATTG
MunI 162 CAATTG C^AATTG
MvnI 4110 CGCG CG^CG
NdeI 3968 CATATG CA^TATG
NruI 4109 TCGCGA TCG^CGA
NsiI 951 ATGCAT ATGCA^T
PmaCI 2954 CACGTG CAC^GTG
PmlI 2954 CACGTG CAC^GTG
PpuMI 2018 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1925 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2424 AACGTT AA^CGTT
RcaI 2131 TCATGA T^CATGA
SbfI 3393 CCTGCAGG CCTGCA^GG
SexAI 3904 ACCAGGT A^CC[AT]GGT
Sse8387I 3393 CCTGCAGG CCTGCA^GG
XmnI 1000 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 495 GGTACC G^GTACC
664 GGTACC
AflIII 2953 ACACGT A^C[GA][CT]GT
4036 ACATGT
Alw44I 1464 GTGCAC G^TGCAC
3178 GTGCAC
ApaI 2291 GGGCCC GGGCC^C
3829 GGGCCC
ApaLI 1464 GTGCAC G^TGCAC
3178 GTGCAC
Asp718I 495 GGTACC G^GTACC
664 GGTACC
AvaI 1313 CCCGAG C^[CT]CG[GA]G
1864 CCCGAG
BalI 2476 TGGCCA TGG^CCA
3689 TGGCCA
BciVI 376 GGATAC GTATCC(6/5)
3422 GTATCC
BclI 1546 TGATCA T^GATCA
2197 TGATCA
BsaI 96 GAGACC GGTCTC(1/5)
720 GAGACC
BsgI 2589 CTGCAC GTGCAG(16/14)
1636 GTGCAG
BsoBI 1313 CCCGAG C^[CT]CG[GA]G
1864 CCCGAG
BstYI 183 GGATCT [GA]^GATC[CT]
2996 AGATCC
DraIII 940 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3835 CACAAAGTG
FauI 3732 CCCGC CCCGC(4/6)
119 GCGGG
FbaI 1546 TGATCA T^GATCA
2197 TGATCA
HaeII 2855 AGCGCC [GA]GCGC^[CT]
3189 GGCGCT
HincII 1569 GTTGAC GT[CT]^[GA]AC
3759 GTCAAC
HindII 1569 GTTGAC GT[CT]^[GA]AC
3759 GTCAAC
KpnI 495 GGTACC GGTAC^C
664 GGTACC
MflI 183 GGATCT [GA]^GATC[CT]
2996 AGATCC
MluNI 2476 TGGCCA TGG^CCA
3689 TGGCCA
MscI 2476 TGGCCA TGG^CCA
3689 TGGCCA
NheI 540 GCTAGC G^CTAGC
935 GCTAGC
PvuII 30 CAGCTG CAG^CTG
2024 CAGCTG
SapI 2273 GAAGAGC GCTCTTC(1/4)
1455 GCTCTTC
TaqI 1691 TCGA T^CGA
3666 TCGA
TthHB8I 1691 TCGA T^CGA
3666 TCGA
XhoII 183 GGATCT [GA]^GATC[CT]
2996 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstXI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI