################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AccI AccIII AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbvCI BfrI BglI BglII BlnI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KasI KspI MluI MroI NaeI NarI NcoI NgoMIV NotI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI PvuI RsrII SacII SalI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 3162 GACGTC GACGT^C AccII 4110 CGCG CG^CG AclI 2424 AACGTT AA^CGTT AcyI 3162 GACGTC G[GA]^CG[CT]C Asp700I 1000 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbrPI 2954 CACGTG CAC^GTG BcgI 3608 CGATTACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2718 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2718 GCTGAGC GC^T[ACGT]AGC BsaAI 2954 CACGTG [CT]AC^GT[GA] BsaHI 3162 GACGTC G[GA]^CG[CT]C BspHI 2131 TCATGA T^CATGA BspLU11I 4036 ACATGT A^CATGT BstAPI 1289 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 4110 CGCG CG^CG CelII 2718 GCTGAGC GC^T[ACGT]AGC DraI 734 TTTAAA TTT^AAA EcoT22I 951 ATGCAT ATGCA^T Hin1I 3162 GACGTC G[GA]^CG[CT]C MfeI 162 CAATTG C^AATTG MunI 162 CAATTG C^AATTG MvnI 4110 CGCG CG^CG NdeI 3968 CATATG CA^TATG NruI 4109 TCGCGA TCG^CGA NsiI 951 ATGCAT ATGCA^T PmaCI 2954 CACGTG CAC^GTG PmlI 2954 CACGTG CAC^GTG PpuMI 2018 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 1925 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2424 AACGTT AA^CGTT RcaI 2131 TCATGA T^CATGA SbfI 3393 CCTGCAGG CCTGCA^GG SexAI 3904 ACCAGGT A^CC[AT]GGT Sse8387I 3393 CCTGCAGG CCTGCA^GG XmnI 1000 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### Acc65I 495 GGTACC G^GTACC 664 GGTACC AflIII 2953 ACACGT A^C[GA][CT]GT 4036 ACATGT Alw44I 1464 GTGCAC G^TGCAC 3178 GTGCAC ApaI 2291 GGGCCC GGGCC^C 3829 GGGCCC ApaLI 1464 GTGCAC G^TGCAC 3178 GTGCAC Asp718I 495 GGTACC G^GTACC 664 GGTACC AvaI 1313 CCCGAG C^[CT]CG[GA]G 1864 CCCGAG BalI 2476 TGGCCA TGG^CCA 3689 TGGCCA BciVI 376 GGATAC GTATCC(6/5) 3422 GTATCC BclI 1546 TGATCA T^GATCA 2197 TGATCA BsaI 96 GAGACC GGTCTC(1/5) 720 GAGACC BsgI 2589 CTGCAC GTGCAG(16/14) 1636 GTGCAG BsoBI 1313 CCCGAG C^[CT]CG[GA]G 1864 CCCGAG BstYI 183 GGATCT [GA]^GATC[CT] 2996 AGATCC DraIII 940 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 3835 CACAAAGTG FauI 3732 CCCGC CCCGC(4/6) 119 GCGGG FbaI 1546 TGATCA T^GATCA 2197 TGATCA HaeII 2855 AGCGCC [GA]GCGC^[CT] 3189 GGCGCT HincII 1569 GTTGAC GT[CT]^[GA]AC 3759 GTCAAC HindII 1569 GTTGAC GT[CT]^[GA]AC 3759 GTCAAC KpnI 495 GGTACC GGTAC^C 664 GGTACC MflI 183 GGATCT [GA]^GATC[CT] 2996 AGATCC MluNI 2476 TGGCCA TGG^CCA 3689 TGGCCA MscI 2476 TGGCCA TGG^CCA 3689 TGGCCA NheI 540 GCTAGC G^CTAGC 935 GCTAGC PvuII 30 CAGCTG CAG^CTG 2024 CAGCTG SapI 2273 GAAGAGC GCTCTTC(1/4) 1455 GCTCTTC TaqI 1691 TCGA T^CGA 3666 TCGA TthHB8I 1691 TCGA T^CGA 3666 TCGA XhoII 183 GGATCT [GA]^GATC[CT] 2996 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BssSI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SphI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI