##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI ApaI AscI AseI
AsnI Asp718I AvaI AviII BalI
BamHI BbrPI BclI BfrI BsaWI
BseAI BsiEI BsiWI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
Cfr10I ClaI CpoI EagI EclXI
Eco52I EcoO65I EcoRI FbaI FseI
FspI HpaI KpnI MfeI MluI
MluNI MroI MscI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 929 GTATAC GT^[AC][GT]AC
AcyI 1172 GGCGCC G[GA]^CG[CT]C
AhdI 2802 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 155 CCTGAGG CC^T[ACGT]AGG
Asp700I 1036 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2802 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2663 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2162 CCTAGG C^CTAGG
BbeI 1172 GGCGCC GGCGC^C
BcgI 2719 GCAGGTGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 705 CCGGG CC^[GC]GG
BglI 3575 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2162 CCTAGG C^CTAGG
BsaHI 1172 GGCGCC G[GA]^CG[CT]C
BsmBI 3459 CGTCTC CGTCTC(1/5)
BsmI 3488 GCATTC GAATGC(1/-1)
BspHI 1044 TCATGA T^CATGA
BsrDI 3651 CATTGC GCAATG(2/0)
BssSI 3544 CACGAG CACGAG(-5/-1)
Bst1107I 929 GTATAC GTA^TAC
BstZ17I 929 GTATAC GTA^TAC
Bsu36I 155 CCTGAGG CC^T[ACGT]AGG
DraIII 1585 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 533 GACGGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 828 TGGCCG [CT]^GGCC[GA]
Eam1105I 2802 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1181 CTCTTC CTCTTC(1/4)
Eco81I 155 CCTGAGG CC^T[ACGT]AGG
EcoRV 2066 GATATC GAT^ATC
EcoT22I 2552 ATGCAT ATGCA^T
HapII 705 CCGG C^CGG
HgaI 39 GCGTC GACGC(5/10)
Hin1I 1172 GGCGCC G[GA]^CG[CT]C
HindIII 3414 AAGCTT A^AGCTT
HpaII 705 CCGG C^CGG
KasI 1172 GGCGCC G^GCGCC
Ksp632I 1181 CTCTTC CTCTTC(1/4)
KspI 3493 CCGCGG CCGC^GG
MspI 705 CCGG C^CGG
NarI 1172 GGCGCC GG^CGCC
NciI 705 CCGGG CC^[GC]GG
NcoI 738 CCATGG C^CATGG
NsiI 2552 ATGCAT ATGCA^T
NspI 2657 GCATGT [GA]CATG^[CT]
PflFI 2663 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1044 TCATGA T^CATGA
SacI 2310 GAGCTC GAGCT^C
SacII 3493 CCGCGG CCGC^GG
SfoI 1172 GGCGCC GGC^GCC
SnaBI 2538 TACGTA TAC^GTA
SspI 662 AATATT AAT^ATT
Tth111I 2663 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1036 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
Alw44I 3217 GTGCAC G^TGCAC
3266 GTGCAC
Aor51HI 1704 AGCGCT AGC^GCT
1711 AGCGCT
ApaLI 3217 GTGCAC G^TGCAC
3266 GTGCAC
BciVI 3295 GTATCC GTATCC(6/5)
3716 GTATCC
BsaAI 2538 TACGTA [CT]AC^GT[GA]
3058 CACGTA
BstXI 1187 CCAGTGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1349 CCATTCCAGTGG
Eco47III 1704 AGCGCT AGC^GCT
1711 AGCGCT
HincII 125 GTCAAC GT[CT]^[GA]AC
1154 GTTGAC
HindII 125 GTCAAC GT[CT]^[GA]AC
1154 GTTGAC
TaqI 456 TCGA T^CGA
876 TCGA
TthHB8I 456 TCGA T^CGA
876 TCGA
XcmI 2275 CCAGTACACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2497 CCACAGATTGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII