################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII AccI AclI AflIII AgeI AhdI Aor51HI ApaI AscI AspEI AspI BbeI BbrPI BclI BsaAI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI HindIII KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 450 TCCGGA T^CCGGA AcyI 5114 GGCGTC G[GA]^CG[CT]C AflII 4602 CTTAAG C^TTAAG AocI 4911 CCTTAGG CC^T[ACGT]AGG AseI 5418 ATTAAT AT^TAAT AsnI 5418 ATTAAT AT^TAAT AvrII 3442 CCTAGG C^CTAGG BcgI 900 GCACACAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 685 GGATAC GTATCC(6/5) BfrI 4602 CTTAAG C^TTAAG BglI 4369 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 4086 AGATCT A^GATCT BlnI 3442 CCTAGG C^CTAGG BsaBI 3713 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 5114 GGCGTC G[GA]^CG[CT]C BsaWI 450 TCCGGA [AT]^CCGG[AT] BseAI 450 TCCGGA T^CCGGA BsiEI 70 CGACCG CG[GA][CT]^CG BsmI 1744 GCATTC GAATGC(1/-1) BspEI 450 TCCGGA T^CCGGA BsrFI 1413 GCCGGC [GA]^CCGG[CT] Bsu36I 4911 CCTTAGG CC^T[ACGT]AGG Cfr10I 1413 GCCGGC [GA]^CCGG[CT] DrdI 2699 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4911 CCTTAGG CC^T[ACGT]AGG HaeII 27 AGCGCC [GA]GCGC^[CT] Hin1I 5114 GGCGTC G[GA]^CG[CT]C MaeII 1109 ACGT A^CGT MamI 3713 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 450 TCCGGA T^CCGGA NaeI 1413 GCCGGC GCC^GGC NgoMIV 1413 GCCGGC G^CCGGC PshBI 5418 ATTAAT AT^TAAT SexAI 2458 ACCTGGT A^CC[AT]GGT SpeI 336 ACTAGT A^CTAGT SspI 4865 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 1870 AAATTC [GA]^AATT[CT] 3148 AAATTC ApoI 1870 AAATTC [GA]^AATT[CT] 3148 AAATTC AviII 3807 TGCGCA TGC^GCA 5136 TGCGCA BamHI 1932 GGATCC G^GATCC 4587 GGATCC BsaI 459 GAGACC GGTCTC(1/5) 3311 GAGACC BspHI 2695 TCATGA T^CATGA 2789 TCATGA BsrDI 3131 GCAATG GCAATG(2/0) 3622 GCAATG EcoT22I 706 ATGCAT ATGCA^T 3830 ATGCAT FspI 3807 TGCGCA TGC^GCA 5136 TGCGCA HincII 2465 GTTAAC GT[CT]^[GA]AC 3352 GTTAAC HindII 2465 GTTAAC GT[CT]^[GA]AC 3352 GTTAAC HpaI 2465 GTTAAC GTT^AAC 3352 GTTAAC NsiI 706 ATGCAT ATGCA^T 3830 ATGCAT RcaI 2695 TCATGA T^CATGA 2789 TCATGA SacI 3174 GAGCTC GAGCT^C 4974 GAGCTC ScaI 929 AGTACT AGT^ACT 3565 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### Acc65I AccII AciI AfaI AluI Alw44I AlwI AlwNI ApaLI Asp700I Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoII XmnI