##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AccI AclI AflIII AgeI
AhdI Aor51HI ApaI AscI AspEI
AspI BbeI BbrPI BclI BsaAI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
HindIII KasI KspI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SwaI
TaqI Tth111I TthHB8I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 450 TCCGGA T^CCGGA
AcyI 5114 GGCGTC G[GA]^CG[CT]C
AflII 4602 CTTAAG C^TTAAG
AocI 4911 CCTTAGG CC^T[ACGT]AGG
AseI 5418 ATTAAT AT^TAAT
AsnI 5418 ATTAAT AT^TAAT
AvrII 3442 CCTAGG C^CTAGG
BcgI 900 GCACACAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 685 GGATAC GTATCC(6/5)
BfrI 4602 CTTAAG C^TTAAG
BglI 4369 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4086 AGATCT A^GATCT
BlnI 3442 CCTAGG C^CTAGG
BsaBI 3713 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 5114 GGCGTC G[GA]^CG[CT]C
BsaWI 450 TCCGGA [AT]^CCGG[AT]
BseAI 450 TCCGGA T^CCGGA
BsiEI 70 CGACCG CG[GA][CT]^CG
BsmI 1744 GCATTC GAATGC(1/-1)
BspEI 450 TCCGGA T^CCGGA
BsrFI 1413 GCCGGC [GA]^CCGG[CT]
Bsu36I 4911 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1413 GCCGGC [GA]^CCGG[CT]
DrdI 2699 GACACCTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4911 CCTTAGG CC^T[ACGT]AGG
HaeII 27 AGCGCC [GA]GCGC^[CT]
Hin1I 5114 GGCGTC G[GA]^CG[CT]C
MaeII 1109 ACGT A^CGT
MamI 3713 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 450 TCCGGA T^CCGGA
NaeI 1413 GCCGGC GCC^GGC
NgoMIV 1413 GCCGGC G^CCGGC
PshBI 5418 ATTAAT AT^TAAT
SexAI 2458 ACCTGGT A^CC[AT]GGT
SpeI 336 ACTAGT A^CTAGT
SspI 4865 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1870 AAATTC [GA]^AATT[CT]
3148 AAATTC
ApoI 1870 AAATTC [GA]^AATT[CT]
3148 AAATTC
AviII 3807 TGCGCA TGC^GCA
5136 TGCGCA
BamHI 1932 GGATCC G^GATCC
4587 GGATCC
BsaI 459 GAGACC GGTCTC(1/5)
3311 GAGACC
BspHI 2695 TCATGA T^CATGA
2789 TCATGA
BsrDI 3131 GCAATG GCAATG(2/0)
3622 GCAATG
EcoT22I 706 ATGCAT ATGCA^T
3830 ATGCAT
FspI 3807 TGCGCA TGC^GCA
5136 TGCGCA
HincII 2465 GTTAAC GT[CT]^[GA]AC
3352 GTTAAC
HindII 2465 GTTAAC GT[CT]^[GA]AC
3352 GTTAAC
HpaI 2465 GTTAAC GTT^AAC
3352 GTTAAC
NsiI 706 ATGCAT ATGCA^T
3830 ATGCAT
RcaI 2695 TCATGA T^CATGA
2789 TCATGA
SacI 3174 GAGCTC GAGCT^C
4974 GAGCTC
ScaI 929 AGTACT AGT^ACT
3565 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
Alw44I AlwI AlwNI ApaLI Asp700I
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XbaI XcmI XhoII XmnI