##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AspEI AspI AviII
BbeI BbrPI BcgI BfrI BlpI
Bpu1102I BsaAI BsaI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII BssSI
BstBI CelII ClaI CpoI Eam1105I
Eco47III EcoT22I FseI FspI HincII
HindII HpaI KasI MluI NarI
NcoI NdeI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 302 GACGTC GACGT^C
AccI 965 GTATAC GT^[AC][GT]AC
AccIII 227 TCCGGA T^CCGGA
AclI 384 AACGTT AA^CGTT
AocI 4139 CCTGAGG CC^T[ACGT]AGG
ApaI 1232 GGGCCC GGGCC^C
AvrII 1775 CCTAGG C^CTAGG
BbvCI 606 CCTCAGC CCTCAGC(-5/-2)
BclI 2023 TGATCA T^GATCA
BglII 2107 AGATCT A^GATCT
BlnI 1775 CCTAGG C^CTAGG
BseAI 227 TCCGGA T^CCGGA
BsmBI 1227 GAGACG CGTCTC(1/5)
BsmI 4657 GAATGC GAATGC(1/-1)
BspEI 227 TCCGGA T^CCGGA
BsrDI 1105 CATTGC GCAATG(2/0)
Bst1107I 965 GTATAC GTA^TAC
BstEII 3587 GGTCACC G^GT[ACGT]ACC
BstPI 3587 GGTCACC G^GT[ACGT]ACC
BstZ17I 965 GTATAC GTA^TAC
Bsu36I 4139 CCTGAGG CC^T[ACGT]AGG
Eco81I 4139 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3587 GGTCACC G^GT[ACGT]ACC
EcoRV 2508 GATATC GAT^ATC
FbaI 2023 TGATCA T^GATCA
HindIII 1887 AAGCTT A^AGCTT
KspI 310 CCGCGG CCGC^GG
MroI 227 TCCGGA T^CCGGA
NaeI 275 GCCGGC GCC^GGC
NgoMIV 275 GCCGGC G^CCGGC
NotI 294 GCGGCCGC GC^GGCCGC
PaeR7I 3804 CTCGAG C^TCGAG
PflMI 1584 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 428 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 384 AACGTT AA^CGTT
SacII 310 CCGCGG CCGC^GG
ScaI 3512 AGTACT AGT^ACT
SphI 2250 GCATGC GCATG^C
SspI 48 AATATT AAT^ATT
StuI 159 AGGCCT AGG^CCT
Van91I 1584 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3804 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 146 GGTACC G^GTACC
930 GGTACC
AseI 1782 ATTAAT AT^TAAT
2621 ATTAAT
AsnI 1782 ATTAAT AT^TAAT
2621 ATTAAT
Asp700I 2826 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4130 GAAGCCCTTC
Asp718I 146 GGTACC G^GTACC
930 GGTACC
AspHI 1293 GAGCTC G[AT]GC[AT]^C
2258 GAGCTC
BamHI 212 GGATCC G^GATCC
230 GGATCC
BciVI 3993 GTATCC GTATCC(6/5)
4560 GTATCC
BglI 432 GCCGTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
731 GCCAGGGTGGC
BsgI 3082 CTGCAC GTGCAG(16/14)
4056 CTGCAC
BsiEI 152 CGGCCG CG[GA][CT]^CG
295 CGGCCG
BsiHKAI 1293 GAGCTC G[AT]GC[AT]^C
2258 GAGCTC
BstXI 3592 CCAAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3613 CCAGGCTGTTGG
DraIII 749 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4761 CACTTTGTG
EagI 152 CGGCCG C^GGCCG
295 CGGCCG
EclXI 152 CGGCCG C^GGCCG
295 CGGCCG
Eco52I 152 CGGCCG C^GGCCG
295 CGGCCG
HaeII 67 GGCGCT [GA]GCGC^[CT]
272 AGCGCC
KpnI 146 GGTACC GGTAC^C
930 GGTACC
MslI 2562 CACTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4862 CACTGTTGTG
PshBI 1782 ATTAAT AT^TAAT
2621 ATTAAT
SacI 1293 GAGCTC GAGCT^C
2258 GAGCTC
SmaI 139 CCCGGG CCC^GGG
203 CCCGGG
SmlI 1359 CTTGAG C^T[CT][GA]AG
3804 CTCGAG
XmaCI 139 CCCGGG C^CCGGG
203 CCCGGG
XmaI 139 CCCGGG C^CCGGG
203 CCCGGG
XmnI 2826 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4130 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII