##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI AgeI AhdI ApaI AscI
AseI AsnI AspEI AviII BbeI
BbrPI BsaAI BsaBI BsiWI BsmBI
BspDI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI DrdI Eam1105I EcoO65I FauI
FseI FspI HpaI KasI KspI
MamI MfeI MluI MunI NarI
NcoI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI SacII SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 923 GACGTC GACGT^C
Acc65I 2985 GGTACC G^GTACC
AccIII 454 TCCGGA T^CCGGA
AflIII 3782 ACACGT A^C[GA][CT]GT
Alw44I 1494 GTGCAC G^TGCAC
AocI 3226 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2259 AGCGCT AGC^GCT
ApaLI 1494 GTGCAC G^TGCAC
Asp718I 2985 GGTACC G^GTACC
AvrII 248 CCTAGG C^CTAGG
BalI 1785 TGGCCA TGG^CCA
BbvCI 2105 CCTCAGC CCTCAGC(-5/-2)
BcgI 2598 CGAAACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3534 GTATCC GTATCC(6/5)
BlnI 248 CCTAGG C^CTAGG
BseAI 454 TCCGGA T^CCGGA
BspEI 454 TCCGGA T^CCGGA
BstBI 1299 TTCGAA TT^CGAA
BstXI 11 CCAGAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3226 CCTCAGG CC^T[ACGT]AGG
CpoI 262 CGGACCG CG^G[AT]CCG
DraI 3951 TTTAAA TTT^AAA
DsaI 2753 CCACGG C^C[GA][CT]GG
EagI 1605 CGGCCG C^GGCCG
EclXI 1605 CGGCCG C^GGCCG
Eco47III 2259 AGCGCT AGC^GCT
Eco52I 1605 CGGCCG C^GGCCG
Eco81I 3226 CCTCAGG CC^T[ACGT]AGG
EcoRV 4287 GATATC GAT^ATC
EcoT22I 3543 ATGCAT ATGCA^T
HaeII 2259 AGCGCT [GA]GCGC^[CT]
KpnI 2985 GGTACC GGTAC^C
MluNI 1785 TGGCCA TGG^CCA
MroI 454 TCCGGA T^CCGGA
MscI 1785 TGGCCA TGG^CCA
NaeI 1603 GCCGGC GCC^GGC
NdeI 2700 CATATG CA^TATG
NgoMIV 1603 GCCGGC G^CCGGC
NheI 2341 GCTAGC G^CTAGC
NsiI 3543 ATGCAT ATGCA^T
NspV 1299 TTCGAA TT^CGAA
PflMI 1160 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 316 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 2027 CGATCG CGAT^CG
RsrII 262 CGGACCG CG^G[AT]CCG
SapI 1472 GAAGAGC GCTCTTC(1/4)
ScaI 2710 AGTACT AGT^ACT
SfuI 1299 TTCGAA TT^CGAA
Van91I 1160 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 619 TCTAGA T^CTAGA
XcmI 2810 CCAAGAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 160 GTCGAC GT^[AC][GT]AC
205 GTCGAC
Asp700I 2212 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2690 GAACTTATTC
AspI 1826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2240 GACAGCGTC
AvaI 365 CCCGAG C^[CT]CG[GA]G
1459 CTCGGG
BclI 1023 TGATCA T^GATCA
1713 TGATCA
BglI 1598 GCCACGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2809 GCCAAGAAGGC
BglII 1908 AGATCT A^GATCT
4031 AGATCT
BsaWI 454 TCCGGA [AT]^CCGG[AT]
3466 ACCGGA
BsiEI 1605 CGGCCG CG[GA][CT]^CG
2027 CGATCG
BsoBI 365 CCCGAG C^[CT]CG[GA]G
1459 CTCGGG
Bsp1407I 2130 TGTACA T^GTACA
3351 TGTACA
BspMI 43 GCAGGT ACCTGC(4/8)
1921 GCAGGT
BsrFI 1603 GCCGGC [GA]^CCGG[CT]
2188 GCCGGT
BsrGI 2130 TGTACA T^GTACA
3351 TGTACA
Cfr10I 1603 GCCGGC [GA]^CCGG[CT]
2188 GCCGGT
EaeI 1605 CGGCCG [CT]^GGCC[GA]
1785 TGGCCA
EcoNI 1875 CCTTAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2215 CCTTTTCCAGG
EcoRI 2740 GAATTC G^AATTC
2760 GAATTC
EcoT14I 248 CCTAGG C^C[AT][AT]GG
1165 CCTTGG
FbaI 1023 TGATCA T^GATCA
1713 TGATCA
NspI 943 GCATGT [GA]CATG^[CT]
2973 ACATGC
PflFI 1826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2240 GACAGCGTC
SalI 160 GTCGAC G^TCGAC
205 GTCGAC
SspBI 2130 TGTACA T^GTACA
3351 TGTACA
StyI 248 CCTAGG C^C[AT][AT]GG
1165 CCTTGG
Tth111I 1826 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2240 GACAGCGTC
XmnI 2212 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2690 GAACTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
AspHI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BfrI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII