##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AscI AseI AsnI AspI
AvrII BbeI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI CelII ClaI CpoI
DraIII DrdI EagI EclXI Eco52I
EcoRV EcoT22I FseI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1769 GGTACC G^GTACC
AhdI 895 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 603 GTGCAC G^TGCAC
Aor51HI 819 AGCGCT AGC^GCT
ApaLI 603 GTGCAC G^TGCAC
Asp700I 2031 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1769 GGTACC G^GTACC
AspEI 895 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1054 TGCGCA TGC^GCA
BbrPI 2569 CACGTG CAC^GTG
BcgI 1668 CGATGGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 256 TGATCA T^GATCA
BglII 12 AGATCT A^GATCT
BsmI 1674 GAATGC GAATGC(1/-1)
BsrBI 3237 GAGCGG CCGCTC(-3/-3)
Bst1107I 1847 GTATAC GTA^TAC
BstAPI 975 GCATACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4008 TTCGAA TT^CGAA
BstEII 680 GGTTACC G^GT[ACGT]ACC
BstPI 680 GGTTACC G^GT[ACGT]ACC
BstZ17I 1847 GTATAC GTA^TAC
DraI 4049 TTTAAA TTT^AAA
Eam1105I 895 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 819 AGCGCT AGC^GCT
EcoO65I 680 GGTTACC G^GT[ACGT]ACC
FbaI 256 TGATCA T^GATCA
FspI 1054 TGCGCA TGC^GCA
HincII 933 GTCAAC GT[CT]^[GA]AC
HindII 933 GTCAAC GT[CT]^[GA]AC
KpnI 1769 GGTACC GGTAC^C
NspV 4008 TTCGAA TT^CGAA
PaeR7I 1048 CTCGAG C^TCGAG
PmaCI 2569 CACGTG CAC^GTG
PmlI 2569 CACGTG CAC^GTG
SapI 551 GCTCTTC GCTCTTC(1/4)
SfuI 4008 TTCGAA TT^CGAA
SphI 1231 GCATGC GCATG^C
SspI 1501 AATATT AAT^ATT
StuI 1306 AGGCCT AGG^CCT
XhoI 1048 CTCGAG C^TCGAG
XmnI 2031 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1065 GTAGAC GT^[AC][GT]AC
1847 GTATAC
AcyI 1104 GACGCC G[GA]^CG[CT]C
2521 GACGCC
AocI 101 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
BalI 3042 TGGCCA TGG^CCA
3538 TGGCCA
BbsI 522 GAAGAC GAAGAC(2/6)
1023 GTCTTC
BglI 210 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
960 GCCTGCCGGGC
BpuAI 522 GAAGAC GAAGAC(2/6)
1023 GTCTTC
BsaAI 462 TACGTG [CT]AC^GT[GA]
2569 CACGTG
BsaHI 1104 GACGCC G[GA]^CG[CT]C
2521 GACGCC
BsaWI 1246 TCCGGT [AT]^CCGG[AT]
1253 TCCGGT
BsiEI 278 CGGTCG CG[GA][CT]^CG
2694 CGACCG
BspMI 834 ACCTGC ACCTGC(4/8)
4035 GCAGGT
BssHII 3985 GCGCGC G^CGCGC
3987 GCGCGC
BssSI 2307 CTCGTG CACGAG(-5/-1)
3271 CTCGTG
Bsu36I 101 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
DsaI 701 CCGTGG C^C[GA][CT]GG
2935 CCGTGG
EarI 552 CTCTTC CTCTTC(1/4)
76 GAAGAG
Eco81I 101 CCTGAGG CC^T[ACGT]AGG
291 CCTGAGG
EcoRI 3492 GAATTC G^AATTC
3663 GAATTC
Hin1I 1104 GACGCC G[GA]^CG[CT]C
2521 GACGCC
Ksp632I 552 CTCTTC CTCTTC(1/4)
76 GAAGAG
MluNI 3042 TGGCCA TGG^CCA
3538 TGGCCA
MscI 3042 TGGCCA TGG^CCA
3538 TGGCCA
MseI 3918 TTAA T^TAA
4050 TTAA
NaeI 1107 GCCGGC GCC^GGC
3627 GCCGGC
NgoMIV 1107 GCCGGC G^CCGGC
3627 GCCGGC
SexAI 2402 ACCAGGT A^CC[AT]GGT
2972 ACCAGGT
Tru9I 3918 TTAA T^TAA
4050 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI