##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Aor51HI AscI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HaeII HgaI
Hin1I KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AclI 4025 AACGTT AA^CGTT
AocI 3769 CCTCAGG CC^T[ACGT]AGG
ApaI 1377 GGGCCC GGGCC^C
AseI 2146 ATTAAT AT^TAAT
AsnI 2146 ATTAAT AT^TAAT
AspI 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 58 GGTGCC G^G[CT][GA]CC
BbvCI 3570 CCTCAGC CCTCAGC(-5/-2)
BcnI 1114 CCCGG CC^[GC]GG
BsgI 743 GTGCAG GTGCAG(16/14)
BspMI 1245 GCAGGT ACCTGC(4/8)
BsrDI 1943 GCAATG GCAATG(2/0)
Bst1107I 4376 GTATAC GTA^TAC
BstXI 1498 CCAGTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4376 GTATAC GTA^TAC
Bsu36I 3769 CCTCAGG CC^T[ACGT]AGG
DraIII 361 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4010 GACCAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3769 CCTCAGG CC^T[ACGT]AGG
EcoNI 1120 CCTTAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 467 GCGGG CCCGC(4/6)
HindIII 2398 AAGCTT A^AGCTT
HpaI 4239 GTTAAC GTT^AAC
MfeI 4322 CAATTG C^AATTG
MunI 4322 CAATTG C^AATTG
NciI 1114 CCCGG CC^[GC]GG
NdeI 3951 CATATG CA^TATG
PflFI 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2146 ATTAAT AT^TAAT
Psp1406I 4025 AACGTT AA^CGTT
SexAI 18 ACCAGGT A^CC[AT]GGT
Tth111I 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflII 2717 CTTAAG C^TTAAG
3311 CTTAAG
AflIII 385 ACATGT A^C[GA][CT]GT
514 ACATGT
Alw44I 2585 GTGCAC G^TGCAC
3553 GTGCAC
AlwNI 1258 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1846 CAGAGACTG
ApaLI 2585 GTGCAC G^TGCAC
3553 GTGCAC
AspHI 2585 GTGCAC G[AT]GC[AT]^C
3553 GTGCAC
BalI 520 TGGCCA TGG^CCA
2414 TGGCCA
BanII 1377 GGGCCC G[GA]GC[CT]^C
3742 GGGCTC
BbsI 197 GAAGAC GAAGAC(2/6)
3596 GAAGAC
BclI 1384 TGATCA T^GATCA
3930 TGATCA
BfrI 2717 CTTAAG C^TTAAG
3311 CTTAAG
BlpI 895 GCTGAGC GC^T[ACGT]AGC
1317 GCTCAGC
Bpu1102I 895 GCTGAGC GC^T[ACGT]AGC
1317 GCTCAGC
BpuAI 197 GAAGAC GAAGAC(2/6)
3596 GAAGAC
BsiHKAI 2585 GTGCAC G[AT]GC[AT]^C
3553 GTGCAC
BsmI 2849 GAATGC GAATGC(1/-1)
4060 GAATGC
BspLU11I 385 ACATGT A^CATGT
514 ACATGT
CelII 895 GCTGAGC GC^T[ACGT]AGC
1317 GCTCAGC
CfoI 1102 GCGC GCG^C
3867 GCGC
EaeI 520 TGGCCA [CT]^GGCC[GA]
2414 TGGCCA
EcoRI 205 GAATTC G^AATTC
2832 GAATTC
EcoT22I 1604 ATGCAT ATGCA^T
3712 ATGCAT
FbaI 1384 TGATCA T^GATCA
3930 TGATCA
HhaI 1102 GCGC GCG^C
3867 GCGC
HinP1I 1102 GCGC G^CGC
3867 GCGC
HincII 1480 GTTGAC GT[CT]^[GA]AC
4239 GTTAAC
HindII 1480 GTTGAC GT[CT]^[GA]AC
4239 GTTAAC
MluNI 520 TGGCCA TGG^CCA
2414 TGGCCA
MscI 520 TGGCCA TGG^CCA
2414 TGGCCA
NheI 1465 GCTAGC G^CTAGC
1725 GCTAGC
NsiI 1604 ATGCAT ATGCA^T
3712 ATGCAT
PshAI 1850 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2740 GACTGCTGTC
PstI 3445 CTGCAG CTGCA^G
4270 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BfaI
BmyI BpmI BsaI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SpeI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII