##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        AhdI        Aor51HI     AscI        Asp700I     
Asp718I     AspEI       AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Cfr10I      ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       FseI        FspI        HaeII       HgaI        
Hin1I       KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NgoMIV      NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SphI        Sse8387I    
SspBI       StuI        SwaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AclI         4025  AACGTT               AA^CGTT
AocI         3769  CCTCAGG              CC^T[ACGT]AGG
ApaI         1377  GGGCCC               GGGCC^C
AseI         2146  ATTAAT               AT^TAAT
AsnI         2146  ATTAAT               AT^TAAT
AspI         3956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI           58  GGTGCC               G^G[CT][GA]CC
BbvCI        3570  CCTCAGC              CCTCAGC(-5/-2)
BcnI         1114  CCCGG                CC^[GC]GG
BsgI          743  GTGCAG               GTGCAG(16/14)
BspMI        1245  GCAGGT               ACCTGC(4/8)
BsrDI        1943  GCAATG               GCAATG(2/0)
Bst1107I     4376  GTATAC               GTA^TAC
BstXI        1498  CCAGTACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      4376  GTATAC               GTA^TAC
Bsu36I       3769  CCTCAGG              CC^T[ACGT]AGG
DraIII        361  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         4010  GACCAAACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3769  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1120  CCTTAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          467  GCGGG                CCCGC(4/6)
HindIII      2398  AAGCTT               A^AGCTT
HpaI         4239  GTTAAC               GTT^AAC
MfeI         4322  CAATTG               C^AATTG
MunI         4322  CAATTG               C^AATTG
NciI         1114  CCCGG                CC^[GC]GG
NdeI         3951  CATATG               CA^TATG
PflFI        3956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2146  ATTAAT               AT^TAAT
Psp1406I     4025  AACGTT               AA^CGTT
SexAI          18  ACCAGGT              A^CC[AT]GGT
Tth111I      3956  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AflII        2717  CTTAAG               C^TTAAG
             3311  CTTAAG              
AflIII        385  ACATGT               A^C[GA][CT]GT
              514  ACATGT              
Alw44I       2585  GTGCAC               G^TGCAC
             3553  GTGCAC              
AlwNI        1258  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1846  CAGAGACTG           
ApaLI        2585  GTGCAC               G^TGCAC
             3553  GTGCAC              
AspHI        2585  GTGCAC               G[AT]GC[AT]^C
             3553  GTGCAC              
BalI          520  TGGCCA               TGG^CCA
             2414  TGGCCA              
BanII        1377  GGGCCC               G[GA]GC[CT]^C
             3742  GGGCTC              
BbsI          197  GAAGAC               GAAGAC(2/6)
             3596  GAAGAC              
BclI         1384  TGATCA               T^GATCA
             3930  TGATCA              
BfrI         2717  CTTAAG               C^TTAAG
             3311  CTTAAG              
BlpI          895  GCTGAGC              GC^T[ACGT]AGC
             1317  GCTCAGC             
Bpu1102I      895  GCTGAGC              GC^T[ACGT]AGC
             1317  GCTCAGC             
BpuAI         197  GAAGAC               GAAGAC(2/6)
             3596  GAAGAC              
BsiHKAI      2585  GTGCAC               G[AT]GC[AT]^C
             3553  GTGCAC              
BsmI         2849  GAATGC               GAATGC(1/-1)
             4060  GAATGC              
BspLU11I      385  ACATGT               A^CATGT
              514  ACATGT              
CelII         895  GCTGAGC              GC^T[ACGT]AGC
             1317  GCTCAGC             
CfoI         1102  GCGC                 GCG^C
             3867  GCGC                
EaeI          520  TGGCCA               [CT]^GGCC[GA]
             2414  TGGCCA              
EcoRI         205  GAATTC               G^AATTC
             2832  GAATTC              
EcoT22I      1604  ATGCAT               ATGCA^T
             3712  ATGCAT              
FbaI         1384  TGATCA               T^GATCA
             3930  TGATCA              
HhaI         1102  GCGC                 GCG^C
             3867  GCGC                
HinP1I       1102  GCGC                 G^CGC
             3867  GCGC                
HincII       1480  GTTGAC               GT[CT]^[GA]AC
             4239  GTTAAC              
HindII       1480  GTTGAC               GT[CT]^[GA]AC
             4239  GTTAAC              
MluNI         520  TGGCCA               TGG^CCA
             2414  TGGCCA              
MscI          520  TGGCCA               TGG^CCA
             2414  TGGCCA              
NheI         1465  GCTAGC               G^CTAGC
             1725  GCTAGC              
NsiI         1604  ATGCAT               ATGCA^T
             3712  ATGCAT              
PshAI        1850  GACTGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2740  GACTGCTGTC          
PstI         3445  CTGCAG               CTGCA^G
             4270  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   90 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaII       BbvI        BfaI        
BmyI        BpmI        BsaI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspHI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SpeI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XbaI        XhoII