################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI Aor51HI AscI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AclI 4025 AACGTT AA^CGTT AocI 3769 CCTCAGG CC^T[ACGT]AGG ApaI 1377 GGGCCC GGGCC^C AseI 2146 ATTAAT AT^TAAT AsnI 2146 ATTAAT AT^TAAT AspI 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 58 GGTGCC G^G[CT][GA]CC BbvCI 3570 CCTCAGC CCTCAGC(-5/-2) BcnI 1114 CCCGG CC^[GC]GG BsgI 743 GTGCAG GTGCAG(16/14) BspMI 1245 GCAGGT ACCTGC(4/8) BsrDI 1943 GCAATG GCAATG(2/0) Bst1107I 4376 GTATAC GTA^TAC BstXI 1498 CCAGTACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4376 GTATAC GTA^TAC Bsu36I 3769 CCTCAGG CC^T[ACGT]AGG DraIII 361 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4010 GACCAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3769 CCTCAGG CC^T[ACGT]AGG EcoNI 1120 CCTTAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 467 GCGGG CCCGC(4/6) HindIII 2398 AAGCTT A^AGCTT HpaI 4239 GTTAAC GTT^AAC MfeI 4322 CAATTG C^AATTG MunI 4322 CAATTG C^AATTG NciI 1114 CCCGG CC^[GC]GG NdeI 3951 CATATG CA^TATG PflFI 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2146 ATTAAT AT^TAAT Psp1406I 4025 AACGTT AA^CGTT SexAI 18 ACCAGGT A^CC[AT]GGT Tth111I 3956 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflII 2717 CTTAAG C^TTAAG 3311 CTTAAG AflIII 385 ACATGT A^C[GA][CT]GT 514 ACATGT Alw44I 2585 GTGCAC G^TGCAC 3553 GTGCAC AlwNI 1258 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1846 CAGAGACTG ApaLI 2585 GTGCAC G^TGCAC 3553 GTGCAC AspHI 2585 GTGCAC G[AT]GC[AT]^C 3553 GTGCAC BalI 520 TGGCCA TGG^CCA 2414 TGGCCA BanII 1377 GGGCCC G[GA]GC[CT]^C 3742 GGGCTC BbsI 197 GAAGAC GAAGAC(2/6) 3596 GAAGAC BclI 1384 TGATCA T^GATCA 3930 TGATCA BfrI 2717 CTTAAG C^TTAAG 3311 CTTAAG BlpI 895 GCTGAGC GC^T[ACGT]AGC 1317 GCTCAGC Bpu1102I 895 GCTGAGC GC^T[ACGT]AGC 1317 GCTCAGC BpuAI 197 GAAGAC GAAGAC(2/6) 3596 GAAGAC BsiHKAI 2585 GTGCAC G[AT]GC[AT]^C 3553 GTGCAC BsmI 2849 GAATGC GAATGC(1/-1) 4060 GAATGC BspLU11I 385 ACATGT A^CATGT 514 ACATGT CelII 895 GCTGAGC GC^T[ACGT]AGC 1317 GCTCAGC CfoI 1102 GCGC GCG^C 3867 GCGC EaeI 520 TGGCCA [CT]^GGCC[GA] 2414 TGGCCA EcoRI 205 GAATTC G^AATTC 2832 GAATTC EcoT22I 1604 ATGCAT ATGCA^T 3712 ATGCAT FbaI 1384 TGATCA T^GATCA 3930 TGATCA HhaI 1102 GCGC GCG^C 3867 GCGC HinP1I 1102 GCGC G^CGC 3867 GCGC HincII 1480 GTTGAC GT[CT]^[GA]AC 4239 GTTAAC HindII 1480 GTTGAC GT[CT]^[GA]AC 4239 GTTAAC MluNI 520 TGGCCA TGG^CCA 2414 TGGCCA MscI 520 TGGCCA TGG^CCA 2414 TGGCCA NheI 1465 GCTAGC G^CTAGC 1725 GCTAGC NsiI 1604 ATGCAT ATGCA^T 3712 ATGCAT PshAI 1850 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2740 GACTGCTGTC PstI 3445 CTGCAG CTGCA^G 4270 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BfaI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI SpeI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XhoII