##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Alw44I Aor51HI ApaLI AscI
AviII BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BssHII BssSI Bst1107I BstAPI BstZ17I
ClaI CpoI DrdI Eco47III EcoRV
FauI FbaI FseI FspI HgaI
KspI MamI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SgrAI SnaBI
Sse8387I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 99 CGCG CG^CG
AcyI 1793 GGCGCC G[GA]^CG[CT]C
AhdI 1906 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1112 ATTAAT AT^TAAT
AsnI 1112 ATTAAT AT^TAAT
AspEI 1906 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1307 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2174 CCTAGG C^CTAGG
BamHI 2069 GGATCC G^GATCC
BbeI 1793 GGCGCC GGCGC^C
BlnI 2174 CCTAGG C^CTAGG
BsaHI 1793 GGCGCC G[GA]^CG[CT]C
BsaI 2138 GAGACC GGTCTC(1/5)
BspHI 1072 TCATGA T^CATGA
BsrBI 3642 CCGCTC CCGCTC(-3/-3)
BsrDI 3649 CATTGC GCAATG(2/0)
BsrFI 2782 ACCGGC [GA]^CCGG[CT]
BstBI 480 TTCGAA TT^CGAA
BstEII 4195 GGTGACC G^GT[ACGT]ACC
BstPI 4195 GGTGACC G^GT[ACGT]ACC
BstUI 99 CGCG CG^CG
Cfr10I 2782 ACCGGC [GA]^CCGG[CT]
EagI 1316 CGGCCG C^GGCCG
Eam1105I 1906 GACCTAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1316 CGGCCG C^GGCCG
Eco52I 1316 CGGCCG C^GGCCG
EcoO65I 4195 GGTGACC G^GT[ACGT]ACC
EcoRI 1419 GAATTC G^AATTC
Hin1I 1793 GGCGCC G[GA]^CG[CT]C
KasI 1793 GGCGCC G^GCGCC
MfeI 1810 CAATTG C^AATTG
MunI 1810 CAATTG C^AATTG
MvnI 99 CGCG CG^CG
NarI 1793 GGCGCC GG^CGCC
NcoI 2192 CCATGG C^CATGG
NspV 480 TTCGAA TT^CGAA
PaeR7I 2007 CTCGAG C^TCGAG
PflFI 1307 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1112 ATTAAT AT^TAAT
PstI 298 CTGCAG CTGCA^G
RcaI 1072 TCATGA T^CATGA
SapI 57 GAAGAGC GCTCTTC(1/4)
SexAI 3663 ACCAGGT A^CC[AT]GGT
SfoI 1793 GGCGCC GGC^GCC
SfuI 480 TTCGAA TT^CGAA
SpeI 2269 ACTAGT A^CTAGT
SphI 321 GCATGC GCATG^C
StuI 340 AGGCCT AGG^CCT
SwaI 1489 ATTTAAAT ATTT^AAAT
Tth111I 1307 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2720 TCTAGA T^CTAGA
XhoI 2007 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2911 GGTACC G^GTACC
3463 GGTACC
AocI 1908 CCTAAGG CC^T[ACGT]AGG
2386 CCTCAGG
ApaI 599 GGGCCC GGGCC^C
2095 GGGCCC
Asp718I 2911 GGTACC G^GTACC
3463 GGTACC
BalI 10 TGGCCA TGG^CCA
2708 TGGCCA
BlpI 874 GCTGAGC GC^T[ACGT]AGC
2580 GCTGAGC
Bpu1102I 874 GCTGAGC GC^T[ACGT]AGC
2580 GCTGAGC
BsaAI 2599 TACGTG [CT]AC^GT[GA]
2623 CACGTA
BsiEI 1316 CGGCCG CG[GA][CT]^CG
2104 CGACCG
BsmBI 629 GAGACG CGTCTC(1/5)
2338 GAGACG
BspLU11I 3518 ACATGT A^CATGT
3820 ACATGT
BstXI 234 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
699 CCAAAGACTTGG
Bsu36I 1908 CCTAAGG CC^T[ACGT]AGG
2386 CCTCAGG
CelII 874 GCTGAGC GC^T[ACGT]AGC
2580 GCTGAGC
DraIII 2596 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
3332 CACAGTGTG
Eco81I 1908 CCTAAGG CC^T[ACGT]AGG
2386 CCTCAGG
EcoT22I 1385 ATGCAT ATGCA^T
3288 ATGCAT
HaeII 1339 AGCGCC [GA]GCGC^[CT]
1793 GGCGCC
HpaI 427 GTTAAC GTT^AAC
2439 GTTAAC
KpnI 2911 GGTACC GGTAC^C
3463 GGTACC
MluNI 10 TGGCCA TGG^CCA
2708 TGGCCA
MscI 10 TGGCCA TGG^CCA
2708 TGGCCA
NsiI 1385 ATGCAT ATGCA^T
3288 ATGCAT
SacI 362 GAGCTC GAGCT^C
2243 GAGCTC
ScaI 1593 AGTACT AGT^ACT
3415 AGTACT
SmaI 2092 CCCGGG CCC^GGG
2614 CCCGGG
SmlI 200 CTCAAG C^T[CT][GA]AG
2007 CTCGAG
XmaCI 2092 CCCGGG C^CCGGG
2614 CCCGGG
XmaI 2092 CCCGGG C^CCGGG
2614 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI