################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI Alw44I ApaLI AscI Asp718I AvaI AviII AvrII BamHI BbeI BbrPI BciVI BclI BlnI BsaAI BsaHI BsiEI BsiWI BsoBI BspDI BsrBI BssHII ClaI CpoI DraIII EagI EclXI Eco52I EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I KasI KpnI KspI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AflIII 3078 ACATGT A^C[GA][CT]GT AhdI 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 380 CCTTAGG CC^T[ACGT]AGG Aor51HI 3659 AGCGCT AGC^GCT ApaI 2568 GGGCCC GGGCC^C Asp700I 3629 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 3108 CCTCAGC CCTCAGC(-5/-2) BcgI 3113 GCACCTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsmBI 2407 GAGACG CGTCTC(1/5) BspLU11I 3078 ACATGT A^CATGT BsrDI 599 CATTGC GCAATG(2/0) Bst1107I 2354 GTATAC GTA^TAC BstXI 2724 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2354 GTATAC GTA^TAC Bsu36I 380 CCTTAGG CC^T[ACGT]AGG Eam1105I 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3659 AGCGCT AGC^GCT Eco81I 380 CCTTAGG CC^T[ACGT]AGG HindIII 3828 AAGCTT A^AGCTT NspI 3078 ACATGT [GA]CATG^[CT] PflFI 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 1007 GAGCTC GAGCT^C SapI 910 GAAGAGC GCTCTTC(1/4) ScaI 855 AGTACT AGT^ACT SspI 3509 AATATT AAT^ATT SwaI 4306 ATTTAAAT ATTT^AAAT Tth111I 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2746 TCTAGA T^CTAGA XmnI 3629 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 44 enzymes) # ################################### AccII 2469 CGCG CG^CG 4227 CGCG AccIII 763 TCCGGA T^CCGGA 3570 TCCGGA AflII 182 CTTAAG C^TTAAG 3481 CTTAAG AseI 549 ATTAAT AT^TAAT 1586 ATTAAT AsnI 549 ATTAAT AT^TAAT 1586 ATTAAT AspHI 6 GAGCAC G[AT]GC[AT]^C 1007 GAGCTC BalI 1671 TGGCCA TGG^CCA 1977 TGGCCA BanI 685 GGCACC G^G[CT][GA]CC 3587 GGCACC BcnI 446 CCGGG CC^[GC]GG 3980 CCCGG BfrI 182 CTTAAG C^TTAAG 3481 CTTAAG BglII 2397 AGATCT A^GATCT 3018 AGATCT BsaBI 1121 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3019 GATCTCAATC BsaI 3896 GAGACC GGTCTC(1/5) 1422 GGTCTC BsaWI 763 TCCGGA [AT]^CCGG[AT] 3570 TCCGGA BseAI 763 TCCGGA T^CCGGA 3570 TCCGGA BsgI 3038 CTGCAC GTGCAG(16/14) 2031 GTGCAG BsiHKAI 6 GAGCAC G[AT]GC[AT]^C 1007 GAGCTC BspEI 763 TCCGGA T^CCGGA 3570 TCCGGA BsrFI 450 GCCGGT [GA]^CCGG[CT] 2607 ACCGGC BstBI 1403 TTCGAA TT^CGAA 1459 TTCGAA BstEII 498 GGTTACC G^GT[ACGT]ACC 3609 GGTAACC BstPI 498 GGTTACC G^GT[ACGT]ACC 3609 GGTAACC BstUI 2469 CGCG CG^CG 4227 CGCG Cfr10I 450 GCCGGT [GA]^CCGG[CT] 2607 ACCGGC DrdI 1895 GACCATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3077 GACATGTTAGTC EaeI 1671 TGGCCA [CT]^GGCC[GA] 1977 TGGCCA EcoO65I 498 GGTTACC G^GT[ACGT]ACC 3609 GGTAACC EcoRI 556 GAATTC G^AATTC 2208 GAATTC HaeII 3467 AGCGCC [GA]GCGC^[CT] 3659 AGCGCT HpaI 243 GTTAAC GTT^AAC 973 GTTAAC MamI 1121 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3019 GATCTCAATC MfeI 1235 CAATTG C^AATTG 2628 CAATTG MluNI 1671 TGGCCA TGG^CCA 1977 TGGCCA MroI 763 TCCGGA T^CCGGA 3570 TCCGGA MscI 1671 TGGCCA TGG^CCA 1977 TGGCCA MslI 1993 CACCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2446 CATCTTTGTG MunI 1235 CAATTG C^AATTG 2628 CAATTG MvnI 2469 CGCG CG^CG 4227 CGCG NciI 446 CCGGG CC^[GC]GG 3980 CCCGG NdeI 1449 CATATG CA^TATG 4437 CATATG NspV 1403 TTCGAA TT^CGAA 1459 TTCGAA PshBI 549 ATTAAT AT^TAAT 1586 ATTAAT SalI 1245 GTCGAC G^TCGAC 2987 GTCGAC SfuI 1403 TTCGAA TT^CGAA 1459 TTCGAA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanII BbsI BbvI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII