##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I ApaLI AscI Asp718I AvaI
AviII AvrII BamHI BbeI BbrPI
BciVI BclI BlnI BsaAI BsaHI
BsiEI BsiWI BsoBI BspDI BsrBI
BssHII ClaI CpoI DraIII EagI
EclXI Eco52I EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I KasI
KpnI KspI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 3078 ACATGT A^C[GA][CT]GT
AhdI 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 380 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3659 AGCGCT AGC^GCT
ApaI 2568 GGGCCC GGGCC^C
Asp700I 3629 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 3108 CCTCAGC CCTCAGC(-5/-2)
BcgI 3113 GCACCTCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmBI 2407 GAGACG CGTCTC(1/5)
BspLU11I 3078 ACATGT A^CATGT
BsrDI 599 CATTGC GCAATG(2/0)
Bst1107I 2354 GTATAC GTA^TAC
BstXI 2724 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2354 GTATAC GTA^TAC
Bsu36I 380 CCTTAGG CC^T[ACGT]AGG
Eam1105I 2292 GACATAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3659 AGCGCT AGC^GCT
Eco81I 380 CCTTAGG CC^T[ACGT]AGG
HindIII 3828 AAGCTT A^AGCTT
NspI 3078 ACATGT [GA]CATG^[CT]
PflFI 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1007 GAGCTC GAGCT^C
SapI 910 GAAGAGC GCTCTTC(1/4)
ScaI 855 AGTACT AGT^ACT
SspI 3509 AATATT AAT^ATT
SwaI 4306 ATTTAAAT ATTT^AAAT
Tth111I 3210 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2746 TCTAGA T^CTAGA
XmnI 3629 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 2469 CGCG CG^CG
4227 CGCG
AccIII 763 TCCGGA T^CCGGA
3570 TCCGGA
AflII 182 CTTAAG C^TTAAG
3481 CTTAAG
AseI 549 ATTAAT AT^TAAT
1586 ATTAAT
AsnI 549 ATTAAT AT^TAAT
1586 ATTAAT
AspHI 6 GAGCAC G[AT]GC[AT]^C
1007 GAGCTC
BalI 1671 TGGCCA TGG^CCA
1977 TGGCCA
BanI 685 GGCACC G^G[CT][GA]CC
3587 GGCACC
BcnI 446 CCGGG CC^[GC]GG
3980 CCCGG
BfrI 182 CTTAAG C^TTAAG
3481 CTTAAG
BglII 2397 AGATCT A^GATCT
3018 AGATCT
BsaBI 1121 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3019 GATCTCAATC
BsaI 3896 GAGACC GGTCTC(1/5)
1422 GGTCTC
BsaWI 763 TCCGGA [AT]^CCGG[AT]
3570 TCCGGA
BseAI 763 TCCGGA T^CCGGA
3570 TCCGGA
BsgI 3038 CTGCAC GTGCAG(16/14)
2031 GTGCAG
BsiHKAI 6 GAGCAC G[AT]GC[AT]^C
1007 GAGCTC
BspEI 763 TCCGGA T^CCGGA
3570 TCCGGA
BsrFI 450 GCCGGT [GA]^CCGG[CT]
2607 ACCGGC
BstBI 1403 TTCGAA TT^CGAA
1459 TTCGAA
BstEII 498 GGTTACC G^GT[ACGT]ACC
3609 GGTAACC
BstPI 498 GGTTACC G^GT[ACGT]ACC
3609 GGTAACC
BstUI 2469 CGCG CG^CG
4227 CGCG
Cfr10I 450 GCCGGT [GA]^CCGG[CT]
2607 ACCGGC
DrdI 1895 GACCATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3077 GACATGTTAGTC
EaeI 1671 TGGCCA [CT]^GGCC[GA]
1977 TGGCCA
EcoO65I 498 GGTTACC G^GT[ACGT]ACC
3609 GGTAACC
EcoRI 556 GAATTC G^AATTC
2208 GAATTC
HaeII 3467 AGCGCC [GA]GCGC^[CT]
3659 AGCGCT
HpaI 243 GTTAAC GTT^AAC
973 GTTAAC
MamI 1121 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3019 GATCTCAATC
MfeI 1235 CAATTG C^AATTG
2628 CAATTG
MluNI 1671 TGGCCA TGG^CCA
1977 TGGCCA
MroI 763 TCCGGA T^CCGGA
3570 TCCGGA
MscI 1671 TGGCCA TGG^CCA
1977 TGGCCA
MslI 1993 CACCGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2446 CATCTTTGTG
MunI 1235 CAATTG C^AATTG
2628 CAATTG
MvnI 2469 CGCG CG^CG
4227 CGCG
NciI 446 CCGGG CC^[GC]GG
3980 CCCGG
NdeI 1449 CATATG CA^TATG
4437 CATATG
NspV 1403 TTCGAA TT^CGAA
1459 TTCGAA
PshBI 549 ATTAAT AT^TAAT
1586 ATTAAT
SalI 1245 GTCGAC G^TCGAC
2987 GTCGAC
SfuI 1403 TTCGAA TT^CGAA
1459 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BanII
BbsI BbvI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspMI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII