##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AhdI Aor51HI ApaI
AscI Asp700I AspEI AspI AvrII
BbeI BbrPI BbvCI BciVI BglI
BlnI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI PacI PflFI PmaCI
PmeI PmlI PpuMI PshAI RsrII
SacII SalI SbfI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI Tth111I XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 62 GGTACC G^GTACC
AccI 976 GTAGAC GT^[AC][GT]AC
AclI 3416 AACGTT AA^CGTT
AflIII 1676 ACATGT A^C[GA][CT]GT
AgeI 48 ACCGGT A^CCGGT
Alw44I 3881 GTGCAC G^TGCAC
ApaLI 3881 GTGCAC G^TGCAC
Asp718I 62 GGTACC G^GTACC
BalI 4737 TGGCCA TGG^CCA
BamHI 3489 GGATCC G^GATCC
BsaAI 4502 TACGTG [CT]AC^GT[GA]
BsiEI 214 CGATCG CG[GA][CT]^CG
BsmI 5124 GAATGC GAATGC(1/-1)
BspLU11I 1676 ACATGT A^CATGT
BstAPI 4298 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 1025 CCATGG C^C[GA][CT]GG
EcoNI 2397 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 2377 AAGCTT A^AGCTT
KpnI 62 GGTACC GGTAC^C
MluNI 4737 TGGCCA TGG^CCA
MscI 4737 TGGCCA TGG^CCA
MspA1I 3853 CAGCTG C[AC]G^C[GT]G
NaeI 145 GCCGGC GCC^GGC
NcoI 1025 CCATGG C^CATGG
NdeI 3342 CATATG CA^TATG
NgoMIV 145 GCCGGC G^CCGGC
PflMI 2803 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 48 ACCGGT A^CCGGT
Psp1406I 3416 AACGTT AA^CGTT
PvuI 214 CGATCG CGAT^CG
PvuII 3853 CAGCTG CAG^CTG
SacI 27 GAGCTC GAGCT^C
SexAI 1659 ACCTGGT A^CC[AT]GGT
SwaI 2697 ATTTAAAT ATTT^AAAT
Van91I 2803 CCAGTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1104 TCTAGA T^CTAGA
XcmI 1261 CCAATTAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 838 GACGCC G[GA]^CG[CT]C
1021 GACGCC
AflII 2827 CTTAAG C^TTAAG
3199 CTTAAG
AlwNI 2804 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3627 CAGGATCTG
AocI 354 CCTGAGG CC^T[ACGT]AGG
2401 CCTGAGG
AspHI 27 GAGCTC G[AT]GC[AT]^C
3881 GTGCAC
AviII 110 TGCGCA TGC^GCA
3402 TGCGCA
BanI 62 GGTACC G^G[CT][GA]CC
4682 GGCACC
BanII 27 GAGCTC G[GA]GC[CT]^C
4706 GAGCCC
BclI 1116 TGATCA T^GATCA
3730 TGATCA
BfrI 2827 CTTAAG C^TTAAG
3199 CTTAAG
BlpI 185 GCTCAGC GC^T[ACGT]AGC
1621 GCTGAGC
Bpu1102I 185 GCTCAGC GC^T[ACGT]AGC
1621 GCTGAGC
BsaHI 838 GACGCC G[GA]^CG[CT]C
1021 GACGCC
BsaWI 48 ACCGGT [AT]^CCGG[AT]
290 ACCGGA
BsiHKAI 27 GAGCTC G[AT]GC[AT]^C
3881 GTGCAC
BsmBI 2175 GAGACG CGTCTC(1/5)
3076 GAGACG
BspHI 1502 TCATGA T^CATGA
3255 TCATGA
BsrBI 394 GAGCGG CCGCTC(-3/-3)
3906 GAGCGG
BsrFI 48 ACCGGT [GA]^CCGG[CT]
145 GCCGGC
BssSI 2363 CTCGTG CACGAG(-5/-1)
3810 CTCGTG
BstBI 1557 TTCGAA TT^CGAA
3330 TTCGAA
BstXI 4326 CCAAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4752 CCAACGAGCTGG
Bsu36I 354 CCTGAGG CC^T[ACGT]AGG
2401 CCTGAGG
CelII 185 GCTCAGC GC^T[ACGT]AGC
1621 GCTGAGC
Cfr10I 48 ACCGGT [GA]^CCGG[CT]
145 GCCGGC
EaeI 143 TGGCCG [CT]^GGCC[GA]
4737 TGGCCA
Eco81I 354 CCTGAGG CC^T[ACGT]AGG
2401 CCTGAGG
FbaI 1116 TGATCA T^GATCA
3730 TGATCA
FspI 110 TGCGCA TGC^GCA
3402 TGCGCA
Hin1I 838 GACGCC G[GA]^CG[CT]C
1021 GACGCC
HincII 1054 GTTGAC GT[CT]^[GA]AC
2116 GTTGAC
HindII 1054 GTTGAC GT[CT]^[GA]AC
2116 GTTGAC
NspV 1557 TTCGAA TT^CGAA
3330 TTCGAA
PaeR7I 194 CTCGAG C^TCGAG
1514 CTCGAG
RcaI 1502 TCATGA T^CATGA
3255 TCATGA
SapI 667 GAAGAGC GCTCTTC(1/4)
3903 GAAGAGC
SfuI 1557 TTCGAA TT^CGAA
3330 TTCGAA
SspI 2086 AATATT AAT^ATT
2529 AATATT
XhoI 194 CTCGAG C^TCGAG
1514 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI AvaI
AvaII BbsI BbvI BcgI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII