##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AclI AflII AocI ApaI
AscI Asp718I BcgI BclI BfrI
BglI BglII BsmI Bsp1407I BspLU11I
BsrGI BssHII BssSI Bsu36I DraIII
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI KpnI MluI NdeI NheI
NruI NsiI NspI PacI PmeI
Psp1406I SalI SbfI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XcmI
###################################
# ONE CUT ENZYMES ( 73 enzymes) #
###################################
AatII 674 GACGTC GACGT^C
AccI 1123 GTATAC GT^[AC][GT]AC
AccIII 406 TCCGGA T^CCGGA
AflIII 3572 ACACGT A^C[GA][CT]GT
AgeI 721 ACCGGT A^CCGGT
AhdI 4019 GACTTACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3363 GTGCAC G^TGCAC
Aor51HI 1996 AGCGCT AGC^GCT
ApaLI 3363 GTGCAC G^TGCAC
Asp700I 618 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4019 GACTTACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2165 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1880 TGCGCA TGC^GCA
AvrII 4149 CCTAGG C^CTAGG
BamHI 2480 GGATCC G^GATCC
BbrPI 3573 CACGTG CAC^GTG
BbvCI 132 CCTCAGC CCTCAGC(-5/-2)
BciVI 2679 GTATCC GTATCC(6/5)
BlnI 4149 CCTAGG C^CTAGG
BlpI 137 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 137 GCTGAGC GC^T[ACGT]AGC
BsaBI 1718 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 406 TCCGGA T^CCGGA
BsiWI 225 CGTACG C^GTACG
BsmBI 349 GAGACG CGTCTC(1/5)
BspDI 451 ATCGAT AT^CGAT
BspEI 406 TCCGGA T^CCGGA
BspHI 1448 TCATGA T^CATGA
Bst1107I 1123 GTATAC GTA^TAC
BstBI 1981 TTCGAA TT^CGAA
BstEII 2143 GGTCACC G^GT[ACGT]ACC
BstPI 2143 GGTCACC G^GT[ACGT]ACC
BstZ17I 1123 GTATAC GTA^TAC
CelII 137 GCTGAGC GC^T[ACGT]AGC
ClaI 451 ATCGAT AT^CGAT
Eam1105I 4019 GACTTACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1996 AGCGCT AGC^GCT
EcoNI 530 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2143 GGTCACC G^GT[ACGT]ACC
FspI 1880 TGCGCA TGC^GCA
HgaI 1571 GACGC GACGC(5/10)
MamI 1718 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3423 CAATTG C^AATTG
MroI 406 TCCGGA T^CCGGA
MunI 3423 CAATTG C^AATTG
NaeI 2603 GCCGGC GCC^GGC
NgoMIV 2603 GCCGGC G^CCGGC
NotI 91 GCGGCCGC GC^GGCCGC
NspV 1981 TTCGAA TT^CGAA
PaeR7I 3626 CTCGAG C^TCGAG
PflFI 2165 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1032 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 721 ACCGGT A^CCGGT
PmaCI 3573 CACGTG CAC^GTG
PmlI 3573 CACGTG CAC^GTG
PshAI 4049 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3694 CTGCAG CTGCA^G
PvuI 453 CGATCG CGAT^CG
RcaI 1448 TCATGA T^CATGA
SapI 2801 GAAGAGC GCTCTTC(1/4)
ScaI 4469 AGTACT AGT^ACT
SexAI 363 ACCAGGT A^CC[AT]GGT
SfuI 1981 TTCGAA TT^CGAA
SmaI 212 CCCGGG CCC^GGG
SspI 4004 AATATT AAT^ATT
StuI 2293 AGGCCT AGG^CCT
Tth111I 2165 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1032 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2015 TCTAGA T^CTAGA
XhoI 3626 CTCGAG C^TCGAG
XmaCI 212 CCCGGG C^CCGGG
XmaI 212 CCCGGG C^CCGGG
XmnI 618 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 1459 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1837 CAGCAGCTG
BalI 3160 TGGCCA TGG^CCA
3391 TGGCCA
BanI 217 GGCGCC G^G[CT][GA]CC
4103 GGCGCC
BbeI 217 GGCGCC GGCGC^C
4103 GGCGCC
BsaI 1975 GGTCTC GGTCTC(1/5)
3007 GGTCTC
CpoI 316 CGGTCCG CG^G[AT]CCG
403 CGGTCCG
HpaI 3254 GTTAAC GTT^AAC
3505 GTTAAC
KasI 217 GGCGCC G^GCGCC
4103 GGCGCC
MluNI 3160 TGGCCA TGG^CCA
3391 TGGCCA
MscI 3160 TGGCCA TGG^CCA
3391 TGGCCA
NarI 217 GGCGCC GG^CGCC
4103 GGCGCC
PvuII 135 CAGCTG CAG^CTG
1840 CAGCTG
RsrII 316 CGGTCCG CG^G[AT]CCG
403 CGGTCCG
SfcI 3694 CTGCAG C^T[GA][CT]AG
4128 CTACAG
SfoI 217 GGCGCC GGC^GCC
4103 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AseI AsnI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PshBI
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII