##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
ApaI AscI Asp718I AviII AvrII
BciVI BlnI BlpI Bpu1102I BsaBI
BsiWI BspLU11I BssHII Bst1107I BstZ17I
CelII CpoI DrdI Eco47III EcoRV
EcoT22I FseI FspI HpaI KpnI
MamI NdeI NheI NruI NsiI
PacI PinAI PmeI Psp1406I PvuI
RsrII SalI ScaI SfiI SnaBI
SphI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccIII 3250 TCCGGA T^CCGGA
AcsI 3120 GAATTC [GA]^AATT[CT]
AflII 1826 CTTAAG C^TTAAG
AflIII 1543 ACGCGT A^C[GA][CT]GT
AlwNI 3585 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3875 CCTCAGG CC^T[ACGT]AGG
ApoI 3120 GAATTC [GA]^AATT[CT]
AseI 4668 ATTAAT AT^TAAT
AsnI 4668 ATTAAT AT^TAAT
Asp700I 4329 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 65 GGATCC G^GATCC
BbeI 34 GGCGCC GGCGC^C
BbvCI 1007 CCTCAGC CCTCAGC(-5/-2)
BfrI 1826 CTTAAG C^TTAAG
BglII 4526 AGATCT A^GATCT
BseAI 3250 TCCGGA T^CCGGA
BsmI 2573 GAATGC GAATGC(1/-1)
BspDI 2781 ATCGAT AT^CGAT
BspEI 3250 TCCGGA T^CCGGA
BsrDI 96 GCAATG GCAATG(2/0)
BssSI 1697 CACGAG CACGAG(-5/-1)
BstAPI 3881 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2516 TTCGAA TT^CGAA
BstXI 3995 CCATTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3875 CCTCAGG CC^T[ACGT]AGG
ClaI 2781 ATCGAT AT^CGAT
DraIII 1645 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 19 CGGCCG C^GGCCG
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
Eco81I 3875 CCTCAGG CC^T[ACGT]AGG
EcoNI 263 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3120 GAATTC G^AATTC
HaeII 34 GGCGCC [GA]GCGC^[CT]
KasI 34 GGCGCC G^GCGCC
KspI 13 CCGCGG CCGC^GG
MfeI 4849 CAATTG C^AATTG
MluI 1543 ACGCGT A^CGCGT
MroI 3250 TCCGGA T^CCGGA
MunI 4849 CAATTG C^AATTG
NarI 34 GGCGCC GG^CGCC
NotI 18 GCGGCCGC GC^GGCCGC
NspV 2516 TTCGAA TT^CGAA
PshBI 4668 ATTAAT AT^TAAT
SacII 13 CCGCGG CCGC^GG
SapI 1583 GCTCTTC GCTCTTC(1/4)
SbfI 351 CCTGCAGG CCTGCA^GG
SexAI 3784 ACCAGGT A^CC[AT]GGT
SfoI 34 GGCGCC GGC^GCC
SfuI 2516 TTCGAA TT^CGAA
SgrAI 36 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3018 CCCGGG CCC^GGG
SpeI 3666 ACTAGT A^CTAGT
Sse8387I 351 CCTGCAGG CCTGCA^GG
StuI 2743 AGGCCT AGG^CCT
XmaCI 3018 CCCGGG C^CCGGG
XmaI 3018 CCCGGG C^CCGGG
XmnI 4329 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1539 GTCTAC GT^[AC][GT]AC
3780 GTCTAC
AspI 2132 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4209 GACAATGTC
BalI 341 TGGCCA TGG^CCA
4369 TGGCCA
BbrPI 168 CACGTG CAC^GTG
3318 CACGTG
BclI 2762 TGATCA T^GATCA
3473 TGATCA
BglI 12 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1084 GCCTGTCCGGC
BsaAI 168 CACGTG [CT]AC^GT[GA]
3318 CACGTG
BsaWI 3232 ACCGGA [AT]^CCGG[AT]
3250 TCCGGA
BsiEI 19 CGGCCG CG[GA][CT]^CG
4234 CGACCG
BsmBI 362 CGTCTC CGTCTC(1/5)
577 CGTCTC
BspHI 658 TCATGA T^CATGA
1609 TCATGA
BsrBI 3012 CCGCTC CCGCTC(-3/-3)
1031 GAGCGG
BstEII 1892 GGTCACC G^GT[ACGT]ACC
3965 GGTAACC
BstPI 1892 GGTCACC G^GT[ACGT]ACC
3965 GGTAACC
EcoO65I 1892 GGTCACC G^GT[ACGT]ACC
3965 GGTAACC
FauI 2286 CCCGC CCCGC(4/6)
2253 GCGGG
FbaI 2762 TGATCA T^GATCA
3473 TGATCA
HincII 680 GTCAAC GT[CT]^[GA]AC
3207 GTCAAC
HindII 680 GTCAAC GT[CT]^[GA]AC
3207 GTCAAC
MluNI 341 TGGCCA TGG^CCA
4369 TGGCCA
MscI 341 TGGCCA TGG^CCA
4369 TGGCCA
NaeI 92 GCCGGC GCC^GGC
2249 GCCGGC
NcoI 3418 CCATGG C^CATGG
3755 CCATGG
NgoMIV 92 GCCGGC G^CCGGC
2249 GCCGGC
PaeR7I 1812 CTCGAG C^TCGAG
2222 CTCGAG
PflFI 2132 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4209 GACAATGTC
PflMI 3285 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4271 CCAGAAAGTGG
PmaCI 168 CACGTG CAC^GTG
3318 CACGTG
PmlI 168 CACGTG CAC^GTG
3318 CACGTG
RcaI 658 TCATGA T^CATGA
1609 TCATGA
SacI 1074 GAGCTC GAGCT^C
2238 GAGCTC
Tth111I 2132 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4209 GACAATGTC
Van91I 3285 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4271 CCAGAAAGTGG
XhoI 1812 CTCGAG C^TCGAG
2222 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI Alw44I AlwI ApaLI AspEI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcgI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eam1105I EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII