################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BbrPI BclI BfrI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI BspDI BsrBI BssHII BstBI CelII ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco52I FbaI FseI FspI HgaI HincII HindII HpaI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SfiI SfuI SgrAI SmaI SnaBI SpeI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 829 TCCGGA T^CCGGA AcyI 2805 GGCGCC G[GA]^CG[CT]C AflIII 3763 ACATGT A^C[GA][CT]GT AvrII 3014 CCTAGG C^CTAGG BamHI 2607 GGATCC G^GATCC BbeI 2805 GGCGCC GGCGC^C BbvCI 368 CCTCAGC CCTCAGC(-5/-2) BlnI 3014 CCTAGG C^CTAGG BsaBI 2420 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2805 GGCGCC G[GA]^CG[CT]C BseAI 829 TCCGGA T^CCGGA BsgI 648 CTGCAC GTGCAG(16/14) BsmI 3471 GCATTC GAATGC(1/-1) BspEI 829 TCCGGA T^CCGGA BspLU11I 3763 ACATGT A^CATGT BsrFI 2093 GCCGGT [GA]^CCGG[CT] BssSI 858 CACGAG CACGAG(-5/-1) BstAPI 2730 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2096 GGTTACC G^GT[ACGT]ACC BstPI 2096 GGTTACC G^GT[ACGT]ACC BstXI 1172 CCAGAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 2093 GCCGGT [GA]^CCGG[CT] EcoO65I 2096 GGTTACC G^GT[ACGT]ACC EcoRV 3131 GATATC GAT^ATC EcoT22I 2812 ATGCAT ATGCA^T Hin1I 2805 GGCGCC G[GA]^CG[CT]C HindIII 1662 AAGCTT A^AGCTT KasI 2805 GGCGCC G^GCGCC MamI 2420 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 829 TCCGGA T^CCGGA NarI 2805 GGCGCC GG^CGCC NcoI 2984 CCATGG C^CATGG NdeI 3353 CATATG CA^TATG NsiI 2812 ATGCAT ATGCA^T PmeI 3767 GTTTAAAC GTTT^AAAC PvuII 131 CAGCTG CAG^CTG SbfI 2936 CCTGCAGG CCTGCA^GG ScaI 3629 AGTACT AGT^ACT SexAI 257 ACCAGGT A^CC[AT]GGT SfoI 2805 GGCGCC GGC^GCC SphI 2472 GCATGC GCATG^C Sse8387I 2936 CCTGCAGG CCTGCA^GG SspI 3335 AATATT AAT^ATT XbaI 3672 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 7 CGCG CG^CG 2130 CGCG AocI 1118 CCTGAGG CC^T[ACGT]AGG 1568 CCTGAGG AvaI 542 CCCGAG C^[CT]CG[GA]G 750 CTCGGG BcgI 3168 CGATGTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3308 GCAGCTAGTTCG BsoBI 542 CCCGAG C^[CT]CG[GA]G 750 CTCGGG Bsp1407I 1099 TGTACA T^GTACA 2650 TGTACA BspHI 3138 TCATGA T^CATGA 3716 TCATGA BsrGI 1099 TGTACA T^GTACA 2650 TGTACA Bst1107I 3563 GTATAC GTA^TAC 3895 GTATAC BstUI 7 CGCG CG^CG 2130 CGCG BstZ17I 3563 GTATAC GTA^TAC 3895 GTATAC Bsu36I 1118 CCTGAGG CC^T[ACGT]AGG 1568 CCTGAGG DraI 3388 TTTAAA TTT^AAA 3768 TTTAAA DrdI 1859 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4163 GACAGTAAGGTC DsaI 81 CCGTGG C^C[GA][CT]GG 2984 CCATGG Eco81I 1118 CCTGAGG CC^T[ACGT]AGG 1568 CCTGAGG EcoRI 2076 GAATTC G^AATTC 3163 GAATTC MspA1I 131 CAGCTG C[AC]G^C[GT]G 2636 CCGCTG MvnI 7 CGCG CG^CG 2130 CGCG RcaI 3138 TCATGA T^CATGA 3716 TCATGA SacI 629 GAGCTC GAGCT^C 2204 GAGCTC SapI 626 GAAGAGC GCTCTTC(1/4) 1549 GCTCTTC SspBI 1099 TGTACA T^GTACA 2650 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI