##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BalI BbrPI BclI BfrI BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmBI
BspDI BsrBI BssHII BstBI CelII
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco52I FbaI FseI
FspI HgaI HincII HindII HpaI
KpnI KspI MfeI MluI MluNI
MscI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 829 TCCGGA T^CCGGA
AcyI 2805 GGCGCC G[GA]^CG[CT]C
AflIII 3763 ACATGT A^C[GA][CT]GT
AvrII 3014 CCTAGG C^CTAGG
BamHI 2607 GGATCC G^GATCC
BbeI 2805 GGCGCC GGCGC^C
BbvCI 368 CCTCAGC CCTCAGC(-5/-2)
BlnI 3014 CCTAGG C^CTAGG
BsaBI 2420 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2805 GGCGCC G[GA]^CG[CT]C
BseAI 829 TCCGGA T^CCGGA
BsgI 648 CTGCAC GTGCAG(16/14)
BsmI 3471 GCATTC GAATGC(1/-1)
BspEI 829 TCCGGA T^CCGGA
BspLU11I 3763 ACATGT A^CATGT
BsrFI 2093 GCCGGT [GA]^CCGG[CT]
BssSI 858 CACGAG CACGAG(-5/-1)
BstAPI 2730 GCAGCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2096 GGTTACC G^GT[ACGT]ACC
BstPI 2096 GGTTACC G^GT[ACGT]ACC
BstXI 1172 CCAGAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2093 GCCGGT [GA]^CCGG[CT]
EcoO65I 2096 GGTTACC G^GT[ACGT]ACC
EcoRV 3131 GATATC GAT^ATC
EcoT22I 2812 ATGCAT ATGCA^T
Hin1I 2805 GGCGCC G[GA]^CG[CT]C
HindIII 1662 AAGCTT A^AGCTT
KasI 2805 GGCGCC G^GCGCC
MamI 2420 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 829 TCCGGA T^CCGGA
NarI 2805 GGCGCC GG^CGCC
NcoI 2984 CCATGG C^CATGG
NdeI 3353 CATATG CA^TATG
NsiI 2812 ATGCAT ATGCA^T
PmeI 3767 GTTTAAAC GTTT^AAAC
PvuII 131 CAGCTG CAG^CTG
SbfI 2936 CCTGCAGG CCTGCA^GG
ScaI 3629 AGTACT AGT^ACT
SexAI 257 ACCAGGT A^CC[AT]GGT
SfoI 2805 GGCGCC GGC^GCC
SphI 2472 GCATGC GCATG^C
Sse8387I 2936 CCTGCAGG CCTGCA^GG
SspI 3335 AATATT AAT^ATT
XbaI 3672 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 7 CGCG CG^CG
2130 CGCG
AocI 1118 CCTGAGG CC^T[ACGT]AGG
1568 CCTGAGG
AvaI 542 CCCGAG C^[CT]CG[GA]G
750 CTCGGG
BcgI 3168 CGATGTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3308 GCAGCTAGTTCG
BsoBI 542 CCCGAG C^[CT]CG[GA]G
750 CTCGGG
Bsp1407I 1099 TGTACA T^GTACA
2650 TGTACA
BspHI 3138 TCATGA T^CATGA
3716 TCATGA
BsrGI 1099 TGTACA T^GTACA
2650 TGTACA
Bst1107I 3563 GTATAC GTA^TAC
3895 GTATAC
BstUI 7 CGCG CG^CG
2130 CGCG
BstZ17I 3563 GTATAC GTA^TAC
3895 GTATAC
Bsu36I 1118 CCTGAGG CC^T[ACGT]AGG
1568 CCTGAGG
DraI 3388 TTTAAA TTT^AAA
3768 TTTAAA
DrdI 1859 GACCAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4163 GACAGTAAGGTC
DsaI 81 CCGTGG C^C[GA][CT]GG
2984 CCATGG
Eco81I 1118 CCTGAGG CC^T[ACGT]AGG
1568 CCTGAGG
EcoRI 2076 GAATTC G^AATTC
3163 GAATTC
MspA1I 131 CAGCTG C[AC]G^C[GT]G
2636 CCGCTG
MvnI 7 CGCG CG^CG
2130 CGCG
RcaI 3138 TCATGA T^CATGA
3716 TCATGA
SacI 629 GAGCTC GAGCT^C
2204 GAGCTC
SapI 626 GAAGAGC GCTCTTC(1/4)
1549 GCTCTTC
SspBI 1099 TGTACA T^GTACA
2650 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApaI ApoI
Asp700I AspHI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI Eco47III
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI