##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BbrPI BbsI BfrI BlnI
BlpI Bpu1102I BpuAI BsaAI BsiWI
BsmBI BspDI BspLU11I Bst1107I BstAPI
BstBI BstXI BstZ17I CelII ClaI
CpoI DrdI Eam1105I Eco47III EcoNI
FseI FspI HindIII HpaI KpnI
MluI NaeI NcoI NdeI NgoMIV
NotI NruI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I Van91I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 744 GACGTC GACGT^C
AocI 82 CCTGAGG CC^T[ACGT]AGG
AspI 35 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1831 CCTCAGC CCTCAGC(-5/-2)
BclI 3531 TGATCA T^GATCA
BsaBI 2313 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1223 GAATGC GAATGC(1/-1)
BsrFI 72 ACCGGC [GA]^CCGG[CT]
BssSI 1337 CTCGTG CACGAG(-5/-1)
BstEII 388 GGTGACC G^GT[ACGT]ACC
BstPI 388 GGTGACC G^GT[ACGT]ACC
Bsu36I 82 CCTGAGG CC^T[ACGT]AGG
Cfr10I 72 ACCGGC [GA]^CCGG[CT]
DraIII 1741 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 82 CCTGAGG CC^T[ACGT]AGG
EcoO65I 388 GGTGACC G^GT[ACGT]ACC
EcoRI 3895 GAATTC G^AATTC
EcoRV 2042 GATATC GAT^ATC
EcoT22I 3931 ATGCAT ATGCA^T
FbaI 3531 TGATCA T^GATCA
HincII 1655 GTTGAC GT[CT]^[GA]AC
HindII 1655 GTTGAC GT[CT]^[GA]AC
MamI 2313 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1938 CAATTG C^AATTG
MunI 1938 CAATTG C^AATTG
NheI 1802 GCTAGC G^CTAGC
NsiI 3931 ATGCAT ATGCA^T
PaeR7I 871 CTCGAG C^TCGAG
PflFI 35 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 185 CTGCAG CTGCA^G
PvuI 960 CGATCG CGAT^CG
PvuII 1788 CAGCTG CAG^CTG
SapI 3796 GAAGAGC GCTCTTC(1/4)
ScaI 2104 AGTACT AGT^ACT
SspI 3693 AATATT AAT^ATT
SwaI 3887 ATTTAAAT ATTT^AAAT
Tth111I 35 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 871 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 924 TCCGGA T^CCGGA
1164 TCCGGA
AflIII 787 ACGTGT A^C[GA][CT]GT
1944 ACGTGT
AseI 2873 ATTAAT AT^TAAT
2924 ATTAAT
AsnI 2873 ATTAAT AT^TAAT
2924 ATTAAT
BalI 1087 TGGCCA TGG^CCA
2796 TGGCCA
BamHI 1161 GGATCC G^GATCC
2531 GGATCC
BcgI 743 CGACGTCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1199 CGAAGTGTCTGC
BciVI 2180 GTATCC GTATCC(6/5)
4511 GTATCC
BglII 2318 AGATCT A^GATCT
4388 AGATCT
BpmI 1381 CTCCAG CTGGAG(16/14)
2816 CTGGAG
BseAI 924 TCCGGA T^CCGGA
1164 TCCGGA
BsgI 2250 CTGCAC GTGCAG(16/14)
4369 CTGCAC
Bsp1407I 2270 TGTACA T^GTACA
2643 TGTACA
BspEI 924 TCCGGA T^CCGGA
1164 TCCGGA
BspMI 1469 ACCTGC ACCTGC(4/8)
1989 ACCTGC
BsrBI 816 CCGCTC CCGCTC(-3/-3)
137 GAGCGG
BsrGI 2270 TGTACA T^GTACA
2643 TGTACA
HgaI 704 GACGC GACGC(5/10)
581 GCGTC
MluNI 1087 TGGCCA TGG^CCA
2796 TGGCCA
MroI 924 TCCGGA T^CCGGA
1164 TCCGGA
MscI 1087 TGGCCA TGG^CCA
2796 TGGCCA
PpuMI 2936 GGGTCCC [GA]G^G[AT]CC[CT]
4224 AGGTCCT
PshBI 2873 ATTAAT AT^TAAT
2924 ATTAAT
SacI 240 GAGCTC GAGCT^C
3106 GAGCTC
SmaI 512 CCCGGG CCC^GGG
693 CCCGGG
SspBI 2270 TGTACA T^GTACA
2643 TGTACA
StuI 2841 AGGCCT AGG^CCT
4080 AGGCCT
XcmI 561 CCAGGCGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2856 CCAGGCTCACTTTGG
XmaCI 512 CCCGGG C^CCGGG
693 CCCGGG
XmaI 512 CCCGGG C^CCGGG
693 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BsaHI BsaI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrDI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII