################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Aor51HI AscI AseI AsnI Asp718I BcgI BclI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrGI BssHII BstBI BstEII BstPI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HpaI KpnI KspI MfeI MluI MroI MunI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfuI SgrAI SnaBI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflII 2647 CTTAAG C^TTAAG AhdI 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3165 TGCGCA TGC^GCA BbeI 2217 GGCGCC GGCGC^C BciVI 982 GGATAC GTATCC(6/5) BfrI 2647 CTTAAG C^TTAAG BlpI 467 GCTGAGC GC^T[ACGT]AGC Bpu1102I 467 GCTGAGC GC^T[ACGT]AGC BsaWI 1363 TCCGGT [AT]^CCGG[AT] BsmBI 3516 CGTCTC CGTCTC(1/5) BspDI 1699 ATCGAT AT^CGAT BspLU11I 1235 ACATGT A^CATGT BsrBI 2415 GAGCGG CCGCTC(-3/-3) BsrDI 3618 CATTGC GCAATG(2/0) BssSI 1742 CACGAG CACGAG(-5/-1) Bst1107I 2723 GTATAC GTA^TAC BstZ17I 2723 GTATAC GTA^TAC CelII 467 GCTGAGC GC^T[ACGT]AGC ClaI 1699 ATCGAT AT^CGAT DraI 2504 TTTAAA TTT^AAA Eam1105I 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2797 GAATTC G^AATTC FspI 3165 TGCGCA TGC^GCA KasI 2217 GGCGCC G^GCGCC NaeI 3674 GCCGGC GCC^GGC NarI 2217 GGCGCC GG^CGCC NgoMIV 3674 GCCGGC G^CCGGC PaeR7I 884 CTCGAG C^TCGAG PflFI 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 4666 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2412 GAAGAGC GCTCTTC(1/4) SbfI 1033 CCTGCAGG CCTGCA^GG ScaI 4830 AGTACT AGT^ACT SfiI 1805 GGCCCTTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2217 GGCGCC GGC^GCC SpeI 1548 ACTAGT A^CTAGT SphI 2540 GCATGC GCATG^C Sse8387I 1033 CCTGCAGG CCTGCA^GG Tth111I 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 884 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 1945 CGCG CG^CG 4725 CGCG Alw44I 896 GTGCAC G^TGCAC 4915 GTGCAC ApaLI 896 GTGCAC G^TGCAC 4915 GTGCAC Asp700I 1245 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5193 GAATAAATTC AvrII 1311 CCTAGG C^CTAGG 4951 CCTAGG BamHI 25 GGATCC G^GATCC 4629 GGATCC BbrPI 408 CACGTG CAC^GTG 3927 CACGTG BglII 1895 AGATCT A^GATCT 2582 AGATCT BlnI 1311 CCTAGG C^CTAGG 4951 CCTAGG BsaBI 3477 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4968 GATGACAATC BsaI 3225 GAGACC GGTCTC(1/5) 433 GGTCTC BstUI 1945 CGCG CG^CG 4725 CGCG BstXI 2554 CCAGAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4098 CCAAGTCAGTGG HincII 1506 GTTGAC GT[CT]^[GA]AC 1588 GTTGAC HindII 1506 GTTGAC GT[CT]^[GA]AC 1588 GTTGAC MamI 3477 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4968 GATGACAATC MvnI 1945 CGCG CG^CG 4725 CGCG NdeI 2352 CATATG CA^TATG 2778 CATATG PmaCI 408 CACGTG CAC^GTG 3927 CACGTG PmlI 408 CACGTG CAC^GTG 3927 CACGTG SexAI 3278 ACCAGGT A^CC[AT]GGT 4616 ACCTGGT XmnI 1245 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 5193 GAATAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI