##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
BcgI BclI BseAI BsiEI BsiWI
Bsp1407I BspEI BspHI BsrGI BssHII
BstBI BstEII BstPI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FbaI FseI HpaI
KpnI KspI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SfuI SgrAI SnaBI SspBI SspI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 2647 CTTAAG C^TTAAG
AhdI 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3165 TGCGCA TGC^GCA
BbeI 2217 GGCGCC GGCGC^C
BciVI 982 GGATAC GTATCC(6/5)
BfrI 2647 CTTAAG C^TTAAG
BlpI 467 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 467 GCTGAGC GC^T[ACGT]AGC
BsaWI 1363 TCCGGT [AT]^CCGG[AT]
BsmBI 3516 CGTCTC CGTCTC(1/5)
BspDI 1699 ATCGAT AT^CGAT
BspLU11I 1235 ACATGT A^CATGT
BsrBI 2415 GAGCGG CCGCTC(-3/-3)
BsrDI 3618 CATTGC GCAATG(2/0)
BssSI 1742 CACGAG CACGAG(-5/-1)
Bst1107I 2723 GTATAC GTA^TAC
BstZ17I 2723 GTATAC GTA^TAC
CelII 467 GCTGAGC GC^T[ACGT]AGC
ClaI 1699 ATCGAT AT^CGAT
DraI 2504 TTTAAA TTT^AAA
Eam1105I 2233 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2797 GAATTC G^AATTC
FspI 3165 TGCGCA TGC^GCA
KasI 2217 GGCGCC G^GCGCC
NaeI 3674 GCCGGC GCC^GGC
NarI 2217 GGCGCC GG^CGCC
NgoMIV 3674 GCCGGC G^CCGGC
PaeR7I 884 CTCGAG C^TCGAG
PflFI 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4666 GACACCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2412 GAAGAGC GCTCTTC(1/4)
SbfI 1033 CCTGCAGG CCTGCA^GG
ScaI 4830 AGTACT AGT^ACT
SfiI 1805 GGCCCTTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2217 GGCGCC GGC^GCC
SpeI 1548 ACTAGT A^CTAGT
SphI 2540 GCATGC GCATG^C
Sse8387I 1033 CCTGCAGG CCTGCA^GG
Tth111I 4096 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 884 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 1945 CGCG CG^CG
4725 CGCG
Alw44I 896 GTGCAC G^TGCAC
4915 GTGCAC
ApaLI 896 GTGCAC G^TGCAC
4915 GTGCAC
Asp700I 1245 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5193 GAATAAATTC
AvrII 1311 CCTAGG C^CTAGG
4951 CCTAGG
BamHI 25 GGATCC G^GATCC
4629 GGATCC
BbrPI 408 CACGTG CAC^GTG
3927 CACGTG
BglII 1895 AGATCT A^GATCT
2582 AGATCT
BlnI 1311 CCTAGG C^CTAGG
4951 CCTAGG
BsaBI 3477 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4968 GATGACAATC
BsaI 3225 GAGACC GGTCTC(1/5)
433 GGTCTC
BstUI 1945 CGCG CG^CG
4725 CGCG
BstXI 2554 CCAGAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4098 CCAAGTCAGTGG
HincII 1506 GTTGAC GT[CT]^[GA]AC
1588 GTTGAC
HindII 1506 GTTGAC GT[CT]^[GA]AC
1588 GTTGAC
MamI 3477 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4968 GATGACAATC
MvnI 1945 CGCG CG^CG
4725 CGCG
NdeI 2352 CATATG CA^TATG
2778 CATATG
PmaCI 408 CACGTG CAC^GTG
3927 CACGTG
PmlI 408 CACGTG CAC^GTG
3927 CACGTG
SexAI 3278 ACCAGGT A^CC[AT]GGT
4616 ACCTGGT
XmnI 1245 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5193 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NheI
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI