##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I AocI ApaI ApaLI AscI
Asp718I AspI AvaI AviII BamHI
BbeI BbrPI BbvCI BcgI BglII
BlpI Bpu1102I BsaAI BsaHI BsiWI
BsmBI BsmI BsoBI BspLU11I BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
Bsu36I CelII CpoI EagI EclXI
Eco52I Eco81I EcoO65I EcoRV FseI
FspI HgaI Hin1I HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 755 CGCG CG^CG
AflII 4791 CTTAAG C^TTAAG
AflIII 62 ACACGT A^C[GA][CT]GT
AhdI 745 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 477 AGCGCT AGC^GCT
AseI 2335 ATTAAT AT^TAAT
AsnI 2335 ATTAAT AT^TAAT
AspEI 745 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 646 CCTAGG C^CTAGG
BalI 384 TGGCCA TGG^CCA
BanI 1460 GGTGCC G^G[CT][GA]CC
BanII 161 GAGCTC G[GA]GC[CT]^C
BciVI 3092 GTATCC GTATCC(6/5)
BcnI 169 CCGGG CC^[GC]GG
BfrI 4791 CTTAAG C^TTAAG
BglI 341 GCCTTAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 646 CCTAGG C^CTAGG
BsaBI 5093 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2059 GGTCTC GGTCTC(1/5)
BsiEI 4999 CGATCG CG[GA][CT]^CG
BspDI 5001 ATCGAT AT^CGAT
BsrBI 1023 CCGCTC CCGCTC(-3/-3)
BsrDI 3142 GCAATG GCAATG(2/0)
BstBI 739 TTCGAA TT^CGAA
BstUI 755 CGCG CG^CG
ClaI 5001 ATCGAT AT^CGAT
DraIII 5154 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 296 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 745 GACAGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 477 AGCGCT AGC^GCT
EcoT22I 2322 ATGCAT ATGCA^T
HindIII 1853 AAGCTT A^AGCTT
MamI 5093 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 384 TGGCCA TGG^CCA
MscI 384 TGGCCA TGG^CCA
MvnI 755 CGCG CG^CG
NciI 169 CCGGG CC^[GC]GG
NdeI 2699 CATATG CA^TATG
NruI 754 TCGCGA TCG^CGA
NsiI 2322 ATGCAT ATGCA^T
NspV 739 TTCGAA TT^CGAA
PflMI 827 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4488 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2335 ATTAAT AT^TAAT
PvuI 4999 CGATCG CGAT^CG
SacI 161 GAGCTC GAGCT^C
SfuI 739 TTCGAA TT^CGAA
SpeI 3679 ACTAGT A^CTAGT
SphI 2447 GCATGC GCATG^C
Van91I 827 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 715 GTCTAC GT^[AC][GT]AC
1486 GTCTAC
AccIII 57 TCCGGA T^CCGGA
1469 TCCGGA
BseAI 57 TCCGGA T^CCGGA
1469 TCCGGA
Bsp1407I 1871 TGTACA T^GTACA
5075 TGTACA
BspEI 57 TCCGGA T^CCGGA
1469 TCCGGA
BspMI 1993 GCAGGT ACCTGC(4/8)
5142 GCAGGT
BsrFI 51 ACCGGC [GA]^CCGG[CT]
682 GCCGGT
BsrGI 1871 TGTACA T^GTACA
5075 TGTACA
BstYI 1572 AGATCC [GA]^GATC[CT]
4354 GGATCT
Cfr10I 51 ACCGGC [GA]^CCGG[CT]
682 GCCGGT
EaeI 384 TGGCCA [CT]^GGCC[GA]
524 CGGCCA
EcoNI 1947 CCTATTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2277 CCTCCTGGAGG
FauI 351 CCCGC CCCGC(4/6)
2862 GCGGG
HaeII 102 AGCGCC [GA]GCGC^[CT]
477 AGCGCT
HincII 2609 GTTGAC GT[CT]^[GA]AC
3466 GTCAAC
HindII 2609 GTTGAC GT[CT]^[GA]AC
3466 GTCAAC
MflI 1572 AGATCC [GA]^GATC[CT]
4354 GGATCT
MroI 57 TCCGGA T^CCGGA
1469 TCCGGA
PpuMI 1444 GGGTCCT [GA]G^G[AT]CC[CT]
2927 AGGTCCT
SmlI 2102 CTTGAG C^T[CT][GA]AG
4791 CTTAAG
SspBI 1871 TGTACA T^GTACA
5075 TGTACA
XcmI 769 CCAAACTGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1566 CCATTCAGATCCTGG
XhoII 1572 AGATCC [GA]^GATC[CT]
4354 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI