##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbeI BbsI BciVI BfrI BglII
BpuAI BsaBI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI Eam1105I EcoRI EcoT22I FseI
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NheI NruI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2521 GTCTAC GT^[AC][GT]AC
AccII 50 CGCG CG^CG
AccIII 3672 TCCGGA T^CCGGA
AcyI 2961 GACGCC G[GA]^CG[CT]C
AflIII 1647 ACGTGT A^C[GA][CT]GT
Aor51HI 686 AGCGCT AGC^GCT
AviII 3804 TGCGCA TGC^GCA
BbrPI 1646 CACGTG CAC^GTG
BcgI 2387 CGACCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1646 CACGTG [CT]AC^GT[GA]
BsaHI 2961 GACGCC G[GA]^CG[CT]C
BsaI 2542 GGTCTC GGTCTC(1/5)
BseAI 3672 TCCGGA T^CCGGA
BsiEI 2498 CGGCCG CG[GA][CT]^CG
BspEI 3672 TCCGGA T^CCGGA
BspHI 3534 TCATGA T^CATGA
BstUI 50 CGCG CG^CG
BstXI 2870 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 3958 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2498 CGGCCG C^GGCCG
EclXI 2498 CGGCCG C^GGCCG
Eco47III 686 AGCGCT AGC^GCT
Eco52I 2498 CGGCCG C^GGCCG
EcoRV 2851 GATATC GAT^ATC
FspI 3804 TGCGCA TGC^GCA
Hin1I 2961 GACGCC G[GA]^CG[CT]C
MroI 3672 TCCGGA T^CCGGA
MvnI 50 CGCG CG^CG
NotI 2497 GCGGCCGC GC^GGCCGC
PmaCI 1646 CACGTG CAC^GTG
PmlI 1646 CACGTG CAC^GTG
RcaI 3534 TCATGA T^CATGA
SexAI 2972 ACCAGGT A^CC[AT]GGT
SphI 2763 GCATGC GCATG^C
SspI 1007 AATATT AAT^ATT
XbaI 990 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTCCGTC
AvrII 3366 CCTAGG C^CTAGG
4068 CCTAGG
BalI 2990 TGGCCA TGG^CCA
4023 TGGCCA
BclI 341 TGATCA T^GATCA
1996 TGATCA
BlnI 3366 CCTAGG C^CTAGG
4068 CCTAGG
BsaWI 679 ACCGGA [AT]^CCGG[AT]
3672 TCCGGA
BsmBI 185 CGTCTC CGTCTC(1/5)
1896 GAGACG
BspMI 2777 ACCTGC ACCTGC(4/8)
2921 ACCTGC
BsrBI 624 GAGCGG CCGCTC(-3/-3)
909 GAGCGG
FbaI 341 TGATCA T^GATCA
1996 TGATCA
HaeII 658 AGCGCC [GA]GCGC^[CT]
686 AGCGCT
HgaI 2509 GACGC GACGC(5/10)
2961 GACGC
HincII 2092 GTTGAC GT[CT]^[GA]AC
2331 GTCAAC
HindII 2092 GTTGAC GT[CT]^[GA]AC
2331 GTCAAC
MaeII 1647 ACGT A^CGT
3783 ACGT
MluNI 2990 TGGCCA TGG^CCA
4023 TGGCCA
MscI 2990 TGGCCA TGG^CCA
4023 TGGCCA
NspI 34 GCATGT [GA]CATG^[CT]
2763 GCATGC
PaeR7I 450 CTCGAG C^TCGAG
558 CTCGAG
PflFI 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTCCGTC
SbfI 731 CCTGCAGG CCTGCA^GG
3743 CCTGCAGG
ScaI 649 AGTACT AGT^ACT
1703 AGTACT
Sse8387I 731 CCTGCAGG CCTGCA^GG
3743 CCTGCAGG
Tth111I 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2826 GACTCCGTC
XhoI 450 CTCGAG C^TCGAG
558 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI