################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI BamHI BbeI BbsI BciVI BfrI BglII BpuAI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoT22I FseI HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NarI NdeI NheI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 2521 GTCTAC GT^[AC][GT]AC AccII 50 CGCG CG^CG AccIII 3672 TCCGGA T^CCGGA AcyI 2961 GACGCC G[GA]^CG[CT]C AflIII 1647 ACGTGT A^C[GA][CT]GT Aor51HI 686 AGCGCT AGC^GCT AviII 3804 TGCGCA TGC^GCA BbrPI 1646 CACGTG CAC^GTG BcgI 2387 CGACCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaAI 1646 CACGTG [CT]AC^GT[GA] BsaHI 2961 GACGCC G[GA]^CG[CT]C BsaI 2542 GGTCTC GGTCTC(1/5) BseAI 3672 TCCGGA T^CCGGA BsiEI 2498 CGGCCG CG[GA][CT]^CG BspEI 3672 TCCGGA T^CCGGA BspHI 3534 TCATGA T^CATGA BstUI 50 CGCG CG^CG BstXI 2870 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 3958 GACATGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2498 CGGCCG C^GGCCG EclXI 2498 CGGCCG C^GGCCG Eco47III 686 AGCGCT AGC^GCT Eco52I 2498 CGGCCG C^GGCCG EcoRV 2851 GATATC GAT^ATC FspI 3804 TGCGCA TGC^GCA Hin1I 2961 GACGCC G[GA]^CG[CT]C MroI 3672 TCCGGA T^CCGGA MvnI 50 CGCG CG^CG NotI 2497 GCGGCCGC GC^GGCCGC PmaCI 1646 CACGTG CAC^GTG PmlI 1646 CACGTG CAC^GTG RcaI 3534 TCATGA T^CATGA SexAI 2972 ACCAGGT A^CC[AT]GGT SphI 2763 GCATGC GCATG^C SspI 1007 AATATT AAT^ATT XbaI 990 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AspI 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2826 GACTCCGTC AvrII 3366 CCTAGG C^CTAGG 4068 CCTAGG BalI 2990 TGGCCA TGG^CCA 4023 TGGCCA BclI 341 TGATCA T^GATCA 1996 TGATCA BlnI 3366 CCTAGG C^CTAGG 4068 CCTAGG BsaWI 679 ACCGGA [AT]^CCGG[AT] 3672 TCCGGA BsmBI 185 CGTCTC CGTCTC(1/5) 1896 GAGACG BspMI 2777 ACCTGC ACCTGC(4/8) 2921 ACCTGC BsrBI 624 GAGCGG CCGCTC(-3/-3) 909 GAGCGG FbaI 341 TGATCA T^GATCA 1996 TGATCA HaeII 658 AGCGCC [GA]GCGC^[CT] 686 AGCGCT HgaI 2509 GACGC GACGC(5/10) 2961 GACGC HincII 2092 GTTGAC GT[CT]^[GA]AC 2331 GTCAAC HindII 2092 GTTGAC GT[CT]^[GA]AC 2331 GTCAAC MaeII 1647 ACGT A^CGT 3783 ACGT MluNI 2990 TGGCCA TGG^CCA 4023 TGGCCA MscI 2990 TGGCCA TGG^CCA 4023 TGGCCA NspI 34 GCATGT [GA]CATG^[CT] 2763 GCATGC PaeR7I 450 CTCGAG C^TCGAG 558 CTCGAG PflFI 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2826 GACTCCGTC SbfI 731 CCTGCAGG CCTGCA^GG 3743 CCTGCAGG ScaI 649 AGTACT AGT^ACT 1703 AGTACT Sse8387I 731 CCTGCAGG CCTGCA^GG 3743 CCTGCAGG Tth111I 2289 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2826 GACTCCGTC XhoI 450 CTCGAG C^TCGAG 558 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AciI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI