##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AlwNI ApaI AscI Asp718I AspEI
AspI AviII BbeI BcgI BsiWI
BspDI BspHI BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraII Eam1105I
EcoNI EcoO109I FspI KasI KpnI
MfeI MluI MunI NarI NdeI
NotI NruI NspV PaeR7I PflFI
PinAI PmeI PpuMI Psp1406I PvuI
RcaI RsrII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 1935 GTAGAC GT^[AC][GT]AC
AccIII 4392 TCCGGA T^CCGGA
Alw44I 543 GTGCAC G^TGCAC
AocI 1505 CCTGAGG CC^T[ACGT]AGG
Aor51HI 159 AGCGCT AGC^GCT
ApaLI 543 GTGCAC G^TGCAC
AseI 1497 ATTAAT AT^TAAT
AsnI 1497 ATTAAT AT^TAAT
AvrII 3560 CCTAGG C^CTAGG
BamHI 817 GGATCC G^GATCC
BanI 609 GGTGCC G^G[CT][GA]CC
BbrPI 77 CACGTG CAC^GTG
BciVI 4572 GGATAC GTATCC(6/5)
BglII 2428 AGATCT A^GATCT
BlnI 3560 CCTAGG C^CTAGG
BlpI 4035 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 4035 GCTTAGC GC^T[ACGT]AGC
BsaAI 77 CACGTG [CT]AC^GT[GA]
BsaI 1841 GGTCTC GGTCTC(1/5)
BseAI 4392 TCCGGA T^CCGGA
BsmBI 1558 CGTCTC CGTCTC(1/5)
BsmI 4363 GAATGC GAATGC(1/-1)
BspEI 4392 TCCGGA T^CCGGA
BspLU11I 2098 ACATGT A^CATGT
BstEII 2675 GGTAACC G^GT[ACGT]ACC
BstPI 2675 GGTAACC G^GT[ACGT]ACC
Bsu36I 1505 CCTGAGG CC^T[ACGT]AGG
CelII 4035 GCTTAGC GC^T[ACGT]AGC
Eco47III 159 AGCGCT AGC^GCT
Eco81I 1505 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2675 GGTAACC G^GT[ACGT]ACC
EcoRV 3592 GATATC GAT^ATC
FseI 175 GGCCGGCC GGCCGG^CC
HincII 4584 GTTAAC GT[CT]^[GA]AC
HindII 4584 GTTAAC GT[CT]^[GA]AC
HindIII 3450 AAGCTT A^AGCTT
HpaI 4584 GTTAAC GTT^AAC
MroI 4392 TCCGGA T^CCGGA
NaeI 176 GCCGGC GCC^GGC
NgoMIV 176 GCCGGC G^CCGGC
NheI 1284 GCTAGC G^CTAGC
PacI 2452 TTAATTAA TTAAT^TAA
PmaCI 77 CACGTG CAC^GTG
PmlI 77 CACGTG CAC^GTG
PshAI 1124 GACGGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1497 ATTAAT AT^TAAT
SacI 3929 GAGCTC GAGCT^C
SexAI 1837 ACCAGGT A^CC[AT]GGT
SmaI 115 CCCGGG CCC^GGG
SpeI 2289 ACTAGT A^CTAGT
SphI 1894 GCATGC GCATG^C
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 1128 GGCGTC G[GA]^CG[CT]C
1332 GACGCC
Asp700I 3666 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3899 GAAAGAATTC
AvaI 67 CTCGGG C^[CT]CG[GA]G
115 CCCGGG
AvaII 152 GGACC G^G[AT]CC
2688 GGACC
BalI 2088 TGGCCA TGG^CCA
4786 TGGCCA
BbvCI 999 CCTCAGC CCTCAGC(-5/-2)
1038 GCTGAGG
BclI 625 TGATCA T^GATCA
3338 TGATCA
BpmI 906 CTGGAG CTGGAG(16/14)
2761 CTGGAG
BsaBI 2086 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2630 GATAGTCATC
BsaHI 1128 GGCGTC G[GA]^CG[CT]C
1332 GACGCC
BsaWI 713 TCCGGT [AT]^CCGG[AT]
4392 TCCGGA
BsgI 2578 GTGCAG GTGCAG(16/14)
2820 GTGCAG
BsoBI 67 CTCGGG C^[CT]CG[GA]G
115 CCCGGG
Bsp1407I 762 TGTACA T^GTACA
3581 TGTACA
BspMI 1939 ACCTGC ACCTGC(4/8)
2737 GCAGGT
BsrFI 176 GCCGGC [GA]^CCGG[CT]
391 ACCGGC
BsrGI 762 TGTACA T^GTACA
3581 TGTACA
BssHII 55 GCGCGC G^CGCGC
486 GCGCGC
BstAPI 1889 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4528 GCAATTTCTGC
Cfr10I 176 GCCGGC [GA]^CCGG[CT]
391 ACCGGC
DraIII 345 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1654 CACGATGTG
EcoT22I 3786 ATGCAT ATGCA^T
3871 ATGCAT
FbaI 625 TGATCA T^GATCA
3338 TGATCA
Hin1I 1128 GGCGTC G[GA]^CG[CT]C
1332 GACGCC
KspI 25 CCGCGG CCGC^GG
240 CCGCGG
MamI 2086 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2630 GATAGTCATC
MluNI 2088 TGGCCA TGG^CCA
4786 TGGCCA
MscI 2088 TGGCCA TGG^CCA
4786 TGGCCA
NcoI 983 CCATGG C^CATGG
2551 CCATGG
NsiI 3786 ATGCAT ATGCA^T
3871 ATGCAT
SacII 25 CCGCGG CCGC^GG
240 CCGCGG
SapI 1398 GAAGAGC GCTCTTC(1/4)
2563 GAAGAGC
SspBI 762 TGTACA T^GTACA
3581 TGTACA
SspI 3457 AATATT AAT^ATT
4224 AATATT
XcmI 340 CCATCCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1900 CCAGCAGGGAGCTGG
XmnI 3666 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3899 GAAAGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AspHI
BanII BbsI BbvI BcnI BfaI
BfrI BglI BmyI BpuAI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DrdI DsaI
EaeI EagI EarI EclXI Eco52I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XhoII