##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
AhdI AocI AscI AspEI AspI
AviII BamHI BbeI BcgI BfrI
BlpI Bpu1102I BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII BssSI Bst1107I BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I FseI FspI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI PacI
PaeR7I PflFI PinAI PmeI PvuI
RsrII SacII SalI SapI ScaI
SfiI SfoI SgrAI SnaBI SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AccII 3074 CGCG CG^CG
AclI 242 AACGTT AA^CGTT
AcyI 2974 GACGCC G[GA]^CG[CT]C
Alw44I 3350 GTGCAC G^TGCAC
AlwI 3538 GATCC GGATC(4/5)
Aor51HI 2234 AGCGCT AGC^GCT
ApaLI 3350 GTGCAC G^TGCAC
AseI 4941 ATTAAT AT^TAAT
AsnI 4941 ATTAAT AT^TAAT
BalI 2858 TGGCCA TGG^CCA
BbrPI 2854 CACGTG CAC^GTG
BbsI 3728 GAAGAC GAAGAC(2/6)
BciVI 1978 GTATCC GTATCC(6/5)
BclI 2999 TGATCA T^GATCA
BglII 4713 AGATCT A^GATCT
BpuAI 3728 GAAGAC GAAGAC(2/6)
BsaAI 2854 CACGTG [CT]AC^GT[GA]
BsaBI 997 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2974 GACGCC G[GA]^CG[CT]C
BsaI 2715 GGTCTC GGTCTC(1/5)
BsaWI 371 TCCGGT [AT]^CCGG[AT]
BsmBI 2815 CGTCTC CGTCTC(1/5)
BspHI 155 TCATGA T^CATGA
BsrBI 205 CCGCTC CCGCTC(-3/-3)
BstBI 1285 TTCGAA TT^CGAA
BstEII 4696 GGTCACC G^GT[ACGT]ACC
BstPI 4696 GGTCACC G^GT[ACGT]ACC
BstUI 3074 CGCG CG^CG
EaeI 2858 TGGCCA [CT]^GGCC[GA]
Eco47III 2234 AGCGCT AGC^GCT
EcoO65I 4696 GGTCACC G^GT[ACGT]ACC
EcoT22I 4092 ATGCAT ATGCA^T
FbaI 2999 TGATCA T^GATCA
Hin1I 2974 GACGCC G[GA]^CG[CT]C
HpaI 582 GTTAAC GTT^AAC
MamI 997 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2858 TGGCCA TGG^CCA
MscI 2858 TGGCCA TGG^CCA
MvnI 3074 CGCG CG^CG
NheI 3768 GCTAGC G^CTAGC
NsiI 4092 ATGCAT ATGCA^T
NspV 1285 TTCGAA TT^CGAA
PflMI 3326 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2854 CACGTG CAC^GTG
PmlI 2854 CACGTG CAC^GTG
PpuMI 2270 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1683 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4941 ATTAAT AT^TAAT
Psp1406I 242 AACGTT AA^CGTT
RcaI 155 TCATGA T^CATGA
SacI 4244 GAGCTC GAGCT^C
SbfI 2026 CCTGCAGG CCTGCA^GG
SexAI 4043 ACCAGGT A^CC[AT]GGT
SfuI 1285 TTCGAA TT^CGAA
SmaI 3143 CCCGGG CCC^GGG
SpeI 3454 ACTAGT A^CTAGT
Sse8387I 2026 CCTGCAGG CCTGCA^GG
SspI 3624 AATATT AAT^ATT
Van91I 3326 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 3143 CCCGGG C^CCGGG
XmaI 3143 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 374 GGTACC G^GTACC
2338 GGTACC
ApaI 3218 GGGCCC GGGCC^C
3252 GGGCCC
Asp718I 374 GGTACC G^GTACC
2338 GGTACC
AvrII 1965 CCTAGG C^CTAGG
2127 CCTAGG
BbvCI 2274 CCTCAGC CCTCAGC(-5/-2)
3307 CCTCAGC
BlnI 1965 CCTAGG C^CTAGG
2127 CCTAGG
BstAPI 659 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3108 GCAGTTCGTGC
BstXI 2087 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3182 CCACTCAGATGG
BstYI 3537 AGATCC [GA]^GATC[CT]
4713 AGATCT
EarI 622 CTCTTC CTCTTC(1/4)
4583 GAAGAG
EcoRI 599 GAATTC G^AATTC
3972 GAATTC
EcoRV 1655 GATATC GAT^ATC
3463 GATATC
HaeII 2234 AGCGCT [GA]GCGC^[CT]
2932 GGCGCT
HindIII 808 AAGCTT A^AGCTT
1573 AAGCTT
KpnI 374 GGTACC GGTAC^C
2338 GGTACC
Ksp632I 622 CTCTTC CTCTTC(1/4)
4583 GAAGAG
MflI 3537 AGATCC [GA]^GATC[CT]
4713 AGATCT
NcoI 707 CCATGG C^CATGG
2087 CCATGG
NdeI 3867 CATATG CA^TATG
4878 CATATG
PvuII 2182 CAGCTG CAG^CTG
3043 CAGCTG
SphI 463 GCATGC GCATG^C
4020 GCATGC
XhoII 3537 AGATCC [GA]^GATC[CT]
4713 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XmnI