################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccI AccIII AflII AgeI AhdI AocI AscI AspEI AspI AviII BamHI BbeI BcgI BfrI BlpI Bpu1102I BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I FseI FspI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI PacI PaeR7I PflFI PinAI PmeI PvuI RsrII SacII SalI SapI ScaI SfiI SfoI SgrAI SnaBI SspBI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccII 3074 CGCG CG^CG AclI 242 AACGTT AA^CGTT AcyI 2974 GACGCC G[GA]^CG[CT]C Alw44I 3350 GTGCAC G^TGCAC AlwI 3538 GATCC GGATC(4/5) Aor51HI 2234 AGCGCT AGC^GCT ApaLI 3350 GTGCAC G^TGCAC AseI 4941 ATTAAT AT^TAAT AsnI 4941 ATTAAT AT^TAAT BalI 2858 TGGCCA TGG^CCA BbrPI 2854 CACGTG CAC^GTG BbsI 3728 GAAGAC GAAGAC(2/6) BciVI 1978 GTATCC GTATCC(6/5) BclI 2999 TGATCA T^GATCA BglII 4713 AGATCT A^GATCT BpuAI 3728 GAAGAC GAAGAC(2/6) BsaAI 2854 CACGTG [CT]AC^GT[GA] BsaBI 997 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2974 GACGCC G[GA]^CG[CT]C BsaI 2715 GGTCTC GGTCTC(1/5) BsaWI 371 TCCGGT [AT]^CCGG[AT] BsmBI 2815 CGTCTC CGTCTC(1/5) BspHI 155 TCATGA T^CATGA BsrBI 205 CCGCTC CCGCTC(-3/-3) BstBI 1285 TTCGAA TT^CGAA BstEII 4696 GGTCACC G^GT[ACGT]ACC BstPI 4696 GGTCACC G^GT[ACGT]ACC BstUI 3074 CGCG CG^CG EaeI 2858 TGGCCA [CT]^GGCC[GA] Eco47III 2234 AGCGCT AGC^GCT EcoO65I 4696 GGTCACC G^GT[ACGT]ACC EcoT22I 4092 ATGCAT ATGCA^T FbaI 2999 TGATCA T^GATCA Hin1I 2974 GACGCC G[GA]^CG[CT]C HpaI 582 GTTAAC GTT^AAC MamI 997 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2858 TGGCCA TGG^CCA MscI 2858 TGGCCA TGG^CCA MvnI 3074 CGCG CG^CG NheI 3768 GCTAGC G^CTAGC NsiI 4092 ATGCAT ATGCA^T NspV 1285 TTCGAA TT^CGAA PflMI 3326 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2854 CACGTG CAC^GTG PmlI 2854 CACGTG CAC^GTG PpuMI 2270 GGGACCT [GA]G^G[AT]CC[CT] PshAI 1683 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4941 ATTAAT AT^TAAT Psp1406I 242 AACGTT AA^CGTT RcaI 155 TCATGA T^CATGA SacI 4244 GAGCTC GAGCT^C SbfI 2026 CCTGCAGG CCTGCA^GG SexAI 4043 ACCAGGT A^CC[AT]GGT SfuI 1285 TTCGAA TT^CGAA SmaI 3143 CCCGGG CCC^GGG SpeI 3454 ACTAGT A^CTAGT Sse8387I 2026 CCTGCAGG CCTGCA^GG SspI 3624 AATATT AAT^ATT Van91I 3326 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 3143 CCCGGG C^CCGGG XmaI 3143 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 374 GGTACC G^GTACC 2338 GGTACC ApaI 3218 GGGCCC GGGCC^C 3252 GGGCCC Asp718I 374 GGTACC G^GTACC 2338 GGTACC AvrII 1965 CCTAGG C^CTAGG 2127 CCTAGG BbvCI 2274 CCTCAGC CCTCAGC(-5/-2) 3307 CCTCAGC BlnI 1965 CCTAGG C^CTAGG 2127 CCTAGG BstAPI 659 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3108 GCAGTTCGTGC BstXI 2087 CCATGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3182 CCACTCAGATGG BstYI 3537 AGATCC [GA]^GATC[CT] 4713 AGATCT EarI 622 CTCTTC CTCTTC(1/4) 4583 GAAGAG EcoRI 599 GAATTC G^AATTC 3972 GAATTC EcoRV 1655 GATATC GAT^ATC 3463 GATATC HaeII 2234 AGCGCT [GA]GCGC^[CT] 2932 GGCGCT HindIII 808 AAGCTT A^AGCTT 1573 AAGCTT KpnI 374 GGTACC GGTAC^C 2338 GGTACC Ksp632I 622 CTCTTC CTCTTC(1/4) 4583 GAAGAG MflI 3537 AGATCC [GA]^GATC[CT] 4713 AGATCT NcoI 707 CCATGG C^CATGG 2087 CCATGG NdeI 3867 CATATG CA^TATG 4878 CATATG PvuII 2182 CAGCTG CAG^CTG 3043 CAGCTG SphI 463 GCATGC GCATG^C 4020 GCATGC XhoII 3537 AGATCC [GA]^GATC[CT] 4713 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI