################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## Acc65I AclI AflII AflIII AgeI AscI AseI AsnI Asp718I AspI AvrII BfrI BglII BlnI BsiEI BsiWI BspLU11I BssHII BstBI CpoI DrdI EagI EclXI Eco52I FseI HindIII HpaI KpnI KspI MluI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfuI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3361 GACGTC GACGT^C AccII 608 CGCG CG^CG AccIII 4743 TCCGGA T^CCGGA Aor51HI 3775 AGCGCT AGC^GCT Asp700I 5004 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2741 TGCGCA TGC^GCA BamHI 780 GGATCC G^GATCC BbrPI 5272 CACGTG CAC^GTG BbvCI 4417 CCTCAGC CCTCAGC(-5/-2) BciVI 3329 GGATAC GTATCC(6/5) BsaAI 5272 CACGTG [CT]AC^GT[GA] BsaBI 834 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4743 TCCGGA [AT]^CCGG[AT] BseAI 4743 TCCGGA T^CCGGA BsmBI 571 GAGACG CGTCTC(1/5) Bsp1407I 1397 TGTACA T^GTACA BspDI 2921 ATCGAT AT^CGAT BspEI 4743 TCCGGA T^CCGGA BsrBI 4601 CCGCTC CCGCTC(-3/-3) BsrFI 524 ACCGGC [GA]^CCGG[CT] BsrGI 1397 TGTACA T^GTACA Bst1107I 4111 GTATAC GTA^TAC BstUI 608 CGCG CG^CG BstZ17I 4111 GTATAC GTA^TAC Cfr10I 524 ACCGGC [GA]^CCGG[CT] ClaI 2921 ATCGAT AT^CGAT DraI 516 TTTAAA TTT^AAA Eco47III 3775 AGCGCT AGC^GCT EcoRI 2854 GAATTC G^AATTC EcoRV 3673 GATATC GAT^ATC EcoT22I 671 ATGCAT ATGCA^T FspI 2741 TGCGCA TGC^GCA MamI 834 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1351 CAATTG C^AATTG MroI 4743 TCCGGA T^CCGGA MunI 1351 CAATTG C^AATTG MvnI 608 CGCG CG^CG NsiI 671 ATGCAT ATGCA^T PmaCI 5272 CACGTG CAC^GTG PmlI 5272 CACGTG CAC^GTG PshAI 5293 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 262 GAAGAGC GCTCTTC(1/4) SfiI 2640 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 5066 CCCGGG CCC^GGG SpeI 510 ACTAGT A^CTAGT SspBI 1397 TGTACA T^GTACA XbaI 4263 TCTAGA T^CTAGA XmaCI 5066 CCCGGG C^CCGGG XmaI 5066 CCCGGG C^CCGGG XmnI 5004 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 2854 GAATTC [GA]^AATT[CT] 3039 GAATTT Alw44I 3483 GTGCAC G^TGCAC 5059 GTGCAC AocI 1546 CCTCAGG CC^T[ACGT]AGG 5043 CCTCAGG ApaI 3550 GGGCCC GGGCC^C 5150 GGGCCC ApaLI 3483 GTGCAC G^TGCAC 5059 GTGCAC ApoI 2854 GAATTC [GA]^AATT[CT] 3039 GAATTT BbeI 3392 GGCGCC GGCGC^C 5287 GGCGCC BbsI 4317 GAAGAC GAAGAC(2/6) 1154 GTCTTC BglI 2641 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5539 GCCAGGGAGGC BpuAI 4317 GAAGAC GAAGAC(2/6) 1154 GTCTTC BssSI 1125 CACGAG CACGAG(-5/-1) 1366 CTCGTG Bsu36I 1546 CCTCAGG CC^T[ACGT]AGG 5043 CCTCAGG Eco81I 1546 CCTCAGG CC^T[ACGT]AGG 5043 CCTCAGG KasI 3392 GGCGCC G^GCGCC 5287 GGCGCC NarI 3392 GGCGCC GG^CGCC 5287 GGCGCC SfoI 3392 GGCGCC GGC^GCC 5287 GGCGCC SspI 119 AATATT AAT^ATT 5648 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccI AciI AcyI AfaI AhdI AluI AlwI AlwNI AspEI AspHI AvaI AvaII BalI BanI BanII BbvI BcgI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BspMI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII