##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AscI AseI AsnI Asp718I AspI
AvrII BfrI BglII BlnI BsiEI
BsiWI BspLU11I BssHII BstBI CpoI
DrdI EagI EclXI Eco52I FseI
HindIII HpaI KpnI KspI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfuI SgrAI SnaBI SphI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3361 GACGTC GACGT^C
AccII 608 CGCG CG^CG
AccIII 4743 TCCGGA T^CCGGA
Aor51HI 3775 AGCGCT AGC^GCT
Asp700I 5004 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2741 TGCGCA TGC^GCA
BamHI 780 GGATCC G^GATCC
BbrPI 5272 CACGTG CAC^GTG
BbvCI 4417 CCTCAGC CCTCAGC(-5/-2)
BciVI 3329 GGATAC GTATCC(6/5)
BsaAI 5272 CACGTG [CT]AC^GT[GA]
BsaBI 834 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4743 TCCGGA [AT]^CCGG[AT]
BseAI 4743 TCCGGA T^CCGGA
BsmBI 571 GAGACG CGTCTC(1/5)
Bsp1407I 1397 TGTACA T^GTACA
BspDI 2921 ATCGAT AT^CGAT
BspEI 4743 TCCGGA T^CCGGA
BsrBI 4601 CCGCTC CCGCTC(-3/-3)
BsrFI 524 ACCGGC [GA]^CCGG[CT]
BsrGI 1397 TGTACA T^GTACA
Bst1107I 4111 GTATAC GTA^TAC
BstUI 608 CGCG CG^CG
BstZ17I 4111 GTATAC GTA^TAC
Cfr10I 524 ACCGGC [GA]^CCGG[CT]
ClaI 2921 ATCGAT AT^CGAT
DraI 516 TTTAAA TTT^AAA
Eco47III 3775 AGCGCT AGC^GCT
EcoRI 2854 GAATTC G^AATTC
EcoRV 3673 GATATC GAT^ATC
EcoT22I 671 ATGCAT ATGCA^T
FspI 2741 TGCGCA TGC^GCA
MamI 834 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1351 CAATTG C^AATTG
MroI 4743 TCCGGA T^CCGGA
MunI 1351 CAATTG C^AATTG
MvnI 608 CGCG CG^CG
NsiI 671 ATGCAT ATGCA^T
PmaCI 5272 CACGTG CAC^GTG
PmlI 5272 CACGTG CAC^GTG
PshAI 5293 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 262 GAAGAGC GCTCTTC(1/4)
SfiI 2640 GGCCACGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 5066 CCCGGG CCC^GGG
SpeI 510 ACTAGT A^CTAGT
SspBI 1397 TGTACA T^GTACA
XbaI 4263 TCTAGA T^CTAGA
XmaCI 5066 CCCGGG C^CCGGG
XmaI 5066 CCCGGG C^CCGGG
XmnI 5004 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 2854 GAATTC [GA]^AATT[CT]
3039 GAATTT
Alw44I 3483 GTGCAC G^TGCAC
5059 GTGCAC
AocI 1546 CCTCAGG CC^T[ACGT]AGG
5043 CCTCAGG
ApaI 3550 GGGCCC GGGCC^C
5150 GGGCCC
ApaLI 3483 GTGCAC G^TGCAC
5059 GTGCAC
ApoI 2854 GAATTC [GA]^AATT[CT]
3039 GAATTT
BbeI 3392 GGCGCC GGCGC^C
5287 GGCGCC
BbsI 4317 GAAGAC GAAGAC(2/6)
1154 GTCTTC
BglI 2641 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5539 GCCAGGGAGGC
BpuAI 4317 GAAGAC GAAGAC(2/6)
1154 GTCTTC
BssSI 1125 CACGAG CACGAG(-5/-1)
1366 CTCGTG
Bsu36I 1546 CCTCAGG CC^T[ACGT]AGG
5043 CCTCAGG
Eco81I 1546 CCTCAGG CC^T[ACGT]AGG
5043 CCTCAGG
KasI 3392 GGCGCC G^GCGCC
5287 GGCGCC
NarI 3392 GGCGCC GG^CGCC
5287 GGCGCC
SfoI 3392 GGCGCC GGC^GCC
5287 GGCGCC
SspI 119 AATATT AAT^ATT
5648 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccI AciI AcyI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcgI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI Eam1105I
EarI EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII